LOV and BLUF flavoproteins’ regulatory photoreceptors of microorganisms and photosensory actuators in optogenetic systems

Fraikin, G. Ya.; Strakhovskaya, M. G.; Belenikina, N. S.; Rubin, Andrey B.

doi:10.3103/s0096392516010028

Cited by 2 publications

(2 citation statements)

References 40 publications

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“…Blue light optogenetic photoswitches based on LOV and BLUF domains have become popular lately outside of plant science in model organisms/systems devoid of blue light sensing (133). LOV and BLUF domains both generally consist of short (100-140 amino acid residues) α/β-modules and undergo conformational changes upon blue light perception, while the photophysical mechanisms differ between them (40). The LOV domain undergoes a reversible photocycle that includes the formation of a thiol adduct between a conserved cysteine residue in the protein and the chromophore.…”

Section: Light-to-signal Conversion Via Photoreceptorsmentioning

confidence: 99%

“…The LOV-based photoreceptors are usually composed of modular sensor and effector domains whose interactions are mediated by the α-helical linker between them (62,118). By contrast, the BLUF photoreceptors mostly mediate their physiological response through a light-induced protein-protein interaction rather than an effector-induced activity change (40).…”

Section: Light-to-signal Conversion Via Photoreceptorsmentioning

confidence: 99%

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Optogenetic Methods in Plant Biology

Konrad

Gao

Zurbriggen

et al. 2023

Annu. Rev. Plant Biol.

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Optogenetics is a technique employing natural or genetically engineered photoreceptors in transgene organisms to manipulate biological activities with light. Light can be turned on or off, and adjusting its intensity and duration allows optogenetic fine-tuning of cellular processes in a noninvasive and spatiotemporally resolved manner. Since the introduction of Channelrhodopsin-2 and phytochrome-based switches nearly 20 years ago, optogenetic tools have been applied in a variety of model organisms with enormous success, but rarely in plants. For a long time, the dependence of plant growth on light and the absence of retinal, the rhodopsin chromophore, prevented the establishment of plant optogenetics until recent progress overcame these difficulties. We summarize the recent results of work in the field to control plant growth and cellular motion via green light–gated ion channels and present successful applications to light-control gene expression with single or combined photoswitches in plants. Furthermore, we highlight the technical requirements and options for future plant optogenetic research.

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Section: Light-to-signal Conversion Via Photoreceptorsmentioning

confidence: 99%

Section: Light-to-signal Conversion Via Photoreceptorsmentioning

confidence: 99%

Optogenetic Methods in Plant Biology

Konrad

Gao

Zurbriggen

et al. 2023

Annu. Rev. Plant Biol.

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Aureochromes – Blue Light Receptors

Matiiv

Chekunova

2018

Biochemistry Moscow

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A variety of living organisms including bacteria, fungi, animals, and plants use blue light (BL) to adapt to changing ambient light. Photosynthetic forms (plants and algae) require energy of light for photosynthesis, movements, development, and regulation of activity. Several complex light-sensitive systems evolved in eukaryotic cells to use the information of light efficiently with photoreceptors selectively absorbing various segments of the solar spectrum, being the first components in the light signal transduction chain. They are most diverse in algae. Photosynthetic stramenopiles, which received chloroplasts from red algae during secondary symbiosis, play an important role in ecosystems and aquaculture, being primary producers. These taxa acquired the ability to use BL for regulation of such processes as phototropism, chloroplast photo-relocation movement, and photomorphogenesis. A new type of BL receptor - aureochrome (AUREO) - was identified in Vaucheria frigida in 2007. AUREO consists of two domains: bZIP (basic-region leucine zipper) domain and LOV (light-oxygen-voltage-sensing) domain, and thus this photoreceptor is a BL-sensitive transcription factor. This review presents current data on the structure, mechanisms of action, and biochemical features of aureochromes.

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LOV and BLUF flavoproteins’ regulatory photoreceptors of microorganisms and photosensory actuators in optogenetic systems

Cited by 2 publications

References 40 publications

Optogenetic Methods in Plant Biology

Optogenetic Methods in Plant Biology

Aureochromes – Blue Light Receptors

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