Lovastatin inhibits its own synthesis in Aspergillus terreus

Abstract

Lovastatin suppresses its own synthesis in the microfungus Aspergillus terreus. The inhibitory effect was documented by spiking identical batch cultures with pure lovastatin (0, 50, 100 and 250 mg/l) 24 h after initiation from spores.

Influence of ultrasound amplitude and duty cycle on fungal morphology and broth rheology of Aspergillus terreus

“…The final biomass concentration was always around 10 g l -1 because of the quantity of nitrogen that was initially added to the medium. In concurrence with data reported previously for this fermentation (Casas López et al 2003, 2004aSainz Herrán et al 2008), the biomass yield on nitrogen was always around 25 g g -1 . Analysis of variance in Table 2 for the effect of sonication on the final biomass concentration confirms that sonication amplitude and cycle did not affect the final concentration (i.e.…”

“…An elevated viscosity impedes oxygen transfer and this has been advanced as an explanation for the low titers of lovastatin during dispersed filamentous growth (Kumar et al 2000). In view of its importance, lovastatin production by A. terreus has been extensively investigated (Novak et al 1997;Szakács et al 1998;Manzoni et al 1998Manzoni et al , 1999Hajjaj et al 2001;Lai et al 2003;Casas López et al 2003, 2004a, b, 2005Rodríguez Porcel et al 2005, 2007, but how ultrasound might influence this fermentation is barely known (Sainz Herrán et al 2008). Some of the published information (Sainz Herrán et al 2008;Casas López et al 2003, 2004b was used in selecting the suitable fermentation conditions for the present study.…”

Effects of the Principal Nutrients on Lovastatin Production byMonascus pilosus

“…similar effect of feedback inhibition has been reported in A. terreus. 16) This productivity was elevated 3-fold, up to 444 mg/l, the highest level reported in liquid culture of wild strains, 9) by appropriate selection of carbon and nitrogen sources with a suitably designed C/N ratio. This highest achieved productivity also indicates the high potential for lovastatin production by M. pilosus, thought so far to have a relatively low ability.…”

Enhancement of Lovastatin Production by Supplementing Polyketide Antibiotics to the Submerged Culture of Aspergillus terreus

“…Supplementation of exogenous lovastatin to the culture of A. terreus would inhibit its own biosynthesis to a certain extent. Increase of additional lovastatin level would lag the startup of its own biosynthesis; however, it did not affect the cell growth [10]. The effects of supplementing exogenous lovastatin on its own biosynthesis and cell growth were investigated in the submerged culture of A. terreus.…”

“…Release of this feedback inhibition may improve the lovastatin production markedly. However, the regulatory mechanism is still not clear [10]. In order to improve the lovastatin production by using the strategy that the feedback regulation occurring in its own biosynthetic pathway is released or reduced, it is necessary to understand the detailed information of this regulatory mechanism.…”