Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)

Srinivasan, Naganandhini; Thangavelu, Kiruthika; Uthandi, Sivakumar

doi:10.1186/s12934-022-01751-2

Cited by 22 publications

(20 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The maximum production of lovastatin of 360 mg L−1 was observed. A. terreus KPR12 produced a maximum lovastatin yield of 450.79 mg L −1 which was reported by Srinivasan et al 33 . Mahmoud and Hadi reported Laetioporus sulphurous which was reported to produce maximum lovastatin of about 280μg/ mL 40 .…”

Section: Discussionsupporting

confidence: 76%

“…This is achieved by reducing the pH and lactonization form. Therefore, producing lovastatin in an acidic medium is required 33 .…”

Section: Discussionmentioning

confidence: 99%

“…Mahmoud and Hadi reported Laetioporus sulphurous which was reported to produce maximum lovastatin of about 280μg/ mL 40 . Pushpa et al reported lovastatin production from various fungi such as Shizophyllum commune and Pleurotusostreatus which produced 38μg/ mL and 30μg/mL, respectively 33 .…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Manogaran¹,

Kannan²,

Narayanan³

et al. 2022

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Lovastatin is a naturally produced 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase enzyme inhibitor- used for treating hypercholesterolemia. It was the first statin drug which was approved by the United States Food and Drug Administration (USFDA). In the current study, endophytic fungus Fusarium nectrioides (MH173849) isolated from Euphorbia hirta L. was used for the production of lovastatin. Four different culture media indicated as M1, M2, M3 and M4 were used for the initial production of lovastatin. Liquid cheese whey was used as nitrogen source. Growth morphology of fungi was investigated using Scanning Electron Microscopy analysis. Also, parameters like temperature, pH, inoculum size, incubation time, and RPM were optimized for the obtaining highest lovastatin production. Among the four media, M4 was found to produce the maximum concentration of lovastatin. Parameters such as temperature of 28°C, pH 6, RPM – 180 rpm and inoculum size of 5 x107 spores/mL were optimal for the production of lovastatin by F. nectrioides (MH173849).

show abstract

Section: Discussionsupporting

confidence: 76%

“…This is achieved by reducing the pH and lactonization form. Therefore, producing lovastatin in an acidic medium is required 33 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Manogaran¹,

Kannan²,

Narayanan³

et al. 2022

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

“…This enzyme was involved in the synthesis of mevalonate, an intermediate metabolite in the synthesis of ergosterol (main components of the fungal membrane) and cholesterol. Thus, since lovastatin has a lipid origin (statin family), it could be related to the ergosterol synthesis pathway [31,43,51,52,55]. Currently, this could be the answer to the increase in lipid production at 96 h of incubation of the A. terreus bio lm in this work.…”

Section: Cns Aspergillosis Is Commonly Reported In Middle Eastern Cou...mentioning

confidence: 83%

“…It was shown that around 24 hours an early maturation occurs, and around 48 and 72 hours respectively, a decrease in the bio lm formed with a statistically representative later elevation was shown. Likewise, it is possible to link a similar behavior to the logarithmic phase and the lag phase (adaptation) in conventional microbial growth kinetics, like that described for A. fumigatus bio lm [41][42][43][44]. The decrease in fungal bio lm formation between 24 and 48 hours (Fig.…”

Section: Cns Aspergillosis Is Commonly Reported In Middle Eastern Cou...mentioning

confidence: 95%

Lipid-Like Biofilm from a Clinical Brain Isolate of Aspergillus terreus: Quantification, Structural Characterization and Stages of the Formation Cycle

et al. 2022

View full text Add to dashboard Cite

Invasive infections caused by lamentous fungi have increased considerably due to the alteration of the host's immune response. Recently, Aspergillus terreus is considered an emerging pathogen and has shown resistance to amphotericin B treatment, resulting in high mortality. The development of fungal bio lm is a virulence factor, and it has been described in some cases of invasive aspergillosis. In addition, although the general composition of fungal bio lms is known, ndings related to bio lms of a lipid nature are rarely reported. In this study, we present the identi cation of a clinical strain of A. terreus by microbiological and molecular tools, also its in vitro bio lm development capacity: i) Bio lm formation was quanti ed by Crystal Violet (CV) and reduction of tetrazolium salts assays (MTT), and simultaneously the stages of bio lm development were described by Scanning Electron Microscopy in High Resolution (SEM-HR). ii) Characterization of the organizational structure of the bio lm was performed by SEM-HR. The hyphal networks developed on the surface, the abundant air channels created between the ECM (extracellular matrix) and the hyphae fused in anastomosis were described. Also, the presence of microhyphae is reported.iii) The chemical composition of the ECM was analyzed by SEM-HR and CLSM (Confocal Laser Scanning Microscopy). Proteins, carbohydrates, nucleic acids and a relevant presence of lipid components were identi ed. Some structures of apparent waxy appearance were highlighted by SEM-HR and backscatterelectron diffraction, for which CLSM was previously performed. To our knowledge, this work is the rst description of a lipid-type bio lm in lamentous fungi, speci cally of the species A. terreus from a clinical isolate.

show abstract

Improving statins production: From non‐genetic strategies to genetic strategies

Fan

Tang

Yang

et al. 2023

Biotechnology Journal

View full text Add to dashboard Cite

Statins are lipid‐lowering drugs that selectively inhibit 3‐hydroxy‐3‐methylglutaryl coenzyme A (HMG‐CoA) reductase, effectively reducing cholesterol synthesis. With improved nutritional conditions, the demand for statins is increasing in the global market. The use of microbial cell factories for statin biosynthesis has become advantageous due to the rapid advancements in biotechnology. These approaches offer simple operation and easy separation of products. This review provides an overview the strategies for statins production via microbial cell factories, including both traditional fermentation culture (non‐genetic) and modern synthetic biology manufacture (genetic). Firstly, the complex fermentation parameters and process control technology on submerged fermentation (SmF) and solid‐state fermentation (SSF) are introduced in detail. The potential use of recoverable agricultural wastes/(biomass) as a fermentation substrate in SSF for statin production is emphasized. Additionally, metabolic engineering strategies for constructing robust engineering strains and directed evolution are also discussed. The review highlights the potential and challenges of using microbial cell factories for statin production, and aims to promote greener production modes for statins.This article is protected by copyright. All rights reserved

show abstract

Lovastatin production by an oleaginous fungus, Aspergillus terreus KPR12 using sago processing wastewater (SWW)

Cited by 22 publications

References 67 publications

Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Lipid-Like Biofilm from a Clinical Brain Isolate of Aspergillus terreus: Quantification, Structural Characterization and Stages of the Formation Cycle

Improving statins production: From non‐genetic strategies to genetic strategies

Contact Info

Product

Resources

About