Low-coherence quantitative differential phase-contrast microscopy using Talbot interferometry

Tajbakhsh, Kiarash; Ebrahimi, Samira; Dashtdar, Masoomeh

doi:10.1364/ao.445369

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…There exist several X-ray PC methods, namely, Talbot interferometry or so called grating-based [32,33], edgeillumination [34], and speckle-based imaging [35]. Propagation-based imaging (PBI) is the most commonly used X-ray PC technique due to its simplicity, and compatibility with a range of facilities.…”

Section: Propagation Based Imagingmentioning

confidence: 99%

A comprehensive study of micro-CT for 3D virtual histology of FFPE tissue blocks

Tajbakhsh,

Neels,

Fadeeva

et al. 2024

Preprint

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Section: Propagation Based Imagingmentioning

confidence: 99%

A comprehensive study of micro-CT for 3D virtual histology of FFPE tissue blocks

Tajbakhsh,

Neels,

Fadeeva

et al. 2024

Preprint

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“…There exist several XPCI methods, namely, Talbot interferometry or so called grating-based [40,41], edgeillumination [42], and speckle-based imaging [43]. Propagation-based imaging (PBI) is the most commonly used XPCI technique due to its simplicity, and compatibility with a range of facilities [44].…”

Section: Propagation-based Imaging On Ffpe Blocksmentioning

confidence: 99%

A Comprehensive Study of Laboratory-Based Micro-CT for 3D Virtual Histology of Human FFPE Tissue Blocks

Tajbakhsh,

Neels,

Fadeeva

et al. 2024

IEEE Access

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Advances in laboratory-based X-ray computed tomography (CT) have enabled X-ray 3D virtual histology. This method shows a great potential as a complementary technique to conventional 2D histology where extensive volumetric sampling is necessary. While formalin-fixed paraffin-embedded (FFPE) tissue blocks are the backbone of clinical histology, there exists no generic optimization, and technical study of the X-ray 3D virtual histology of FFPE blocks. X-ray micro-CT of FFPE blocks is studied and optimized in their native state within the cassette to minimize the interference of X-ray 3D virtual histology with clinical workflows and standards, hence facilitating the technology transfer to the clinics. The optimization is carried on the sample positioning, tungsten tubes acceleration voltage, and artifact reduction. Then propagation-based imaging of FFPE blocks is extensively discussed. Hierarchical (local) tomography and laminography are presented as viable approaches for achieving higher spatial resolutions. In the end, future perspectives are given by considering state-of-the-art micro-CT scanners using liquid-metal-jet sources, large-area detectors, and photon counting detectors. The results achieved here are generic and can be applicable to any laboratory-based scanner with a tungsten target source and cone-beam geometry. This article provides a starting point for anyone new to X-ray 3D virtual histology on FFPE blocks, but also serves as a useful source for more experienced users.INDEX TERMS 3D virtual histology, computed tomography, formalin-fixed paraffin-embedded blocks, phase-contrast imaging, propagation-based imaging, transport of intensity equation, X-ray imaging.

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“…Digital holography (DH) has become a rapidly developing technique for 3D shape measurement in recent years and is increasingly used in the fields of microscopic imaging [1,2], microstructure surface measurement [3], and living cell analysis [4,5]. Slightly off-axis digital holography (SODH) has gained increased attention because it is faster than on-axis DH in reconstructing images and achieves better resolution of reconstructed images than off-axis DH.…”

Section: Introductionmentioning

confidence: 99%

Slightly Off-Axis Digital Holography Using a Transmission Grating and GPU-Accelerated Parallel Phase Reconstruction

Bai,

Chen,

Sun

et al. 2023

Photonics

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Slightly off-axis digital holography is proposed using transmission grating to obtain quantitative phase distribution. The experimental device is based on an improved 4f optical system in which a two-window input plane is used to form the object beam and reference beam. Then, the two beams are diffracted into multiple orders by the transmission grating placed at the Fourier plane. By applying a modified Michelson configuration, the interference patterns can be generated by the object and reference beams from different diffraction orders. After translating the grating, a random phase shift can be introduced to the hologram. To demonstrate the feasibility of our method, both thick and thin phase specimens are retrieved using two carrier phase-shifting holograms. Furthermore, we use the phase reconstruction algorithm based on the NVIDIA CUDA programming model to reduce the retrieval time. Meanwhile, we optimize the discrete cosine transform (DCT)-based least-squares unwrapping algorithm to unwrap the phase. By porting the entire phase reconstruction process to the graphics processing unit (GPU), the phase retrieval acceleration and execution efficiency significantly improve. To demonstrate the feasibility of our method, it is found that our method can measure the surface profiles of standard elements, such as a plano-convex cylinder lens and a microlens array, with a relative error of about 0.5%. For holograms with a different phase shift, the root-mean-square (RMS) value of the phase difference for the main imaging region is about 0.2 rad. By accelerating the phase reconstruction with GPU implementation, a speedup ratio of about 20× for the thick phase specimen and a speedup ratio of about 15× for the thin-phase specimen can be obtained for holograms with a pixel size of 1024 × 1024.

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Low-coherence quantitative differential phase-contrast microscopy using Talbot interferometry

Cited by 8 publications

References 31 publications

A comprehensive study of micro-CT for 3D virtual histology of FFPE tissue blocks

A comprehensive study of micro-CT for 3D virtual histology of FFPE tissue blocks

A Comprehensive Study of Laboratory-Based Micro-CT for 3D Virtual Histology of Human FFPE Tissue Blocks

Slightly Off-Axis Digital Holography Using a Transmission Grating and GPU-Accelerated Parallel Phase Reconstruction

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