Low concentration of MeJA-elicited the positive growth of Rosa Roxburghii via balancing phytohormone signal transduction and triterpenoids synthesis

Ning, Yuan; Ding, Yun-kun; Chang, Yuan‐Hang; Zhang, Su; An, Hongyu; Fu, Yu-jie

doi:10.1007/s10725-023-01012-1

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…Gaertn., Betula platyphylla Sukaczev, Ocimum basilicum L., and Withania somnifera (L.) Dunal ( Misra et al., 2017 ; Faizal and Sari, 2019 ; Sharma et al., 2019 ; Yin et al., 2020 ; Chen et al., 2023 ). In this process, the activities of key enzymes involved in triterpenoid biosynthesis and the expression levels of related genes were affected by the MeJA concentration ( Yin et al., 2020 ; Dinday and Ghosh, 2023 ; Li et al., 2023 ; Ning et al., 2023 ; Xia Q. et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Dai,

Zhang,

et al. 2024

Front. Plant Sci.

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Panax notoginseng is a highly valued perennial medicinal herb plant in Yunnan Province, China, and the taproots are the main medicinal parts that are rich in active substances of P. notoginseng saponins. The main purpose of this study is to uncover the physiological and molecular mechanism of Panax notoginseng saponin accumulation triggered by methyl jasmonate (MeJA) under arbuscular mycorrhizal fungi (AMF) by determining physiological indices, high-throughput sequencing and correlation analysis. Physiological results showed that the biomass and saponin contents of P. notoginseng, the concentrations of jasmonic acids (JAs) and the key enzyme activities involved in notoginsenoside biosynthesis significantly increased under AMF or MeJA, but the interactive treatment of AMF and MeJA weakened the effect of AMF, suggesting that a high concentration of endogenous JA have inhibitory effect. Transcriptome sequencing results indicated that differential expressed genes (DEGs) involved in notoginsenoside and JA biosynthesis were significantly enriched in response to AMF induction, e.g., upregulated genes of diphosphocytidyl-2-C-methyl-d-erythritol kinases (ISPEs), cytochrome P450 monooxygenases (CYP450s)_and glycosyltransferases (GTs), while treatments AMF-MeJA and salicylhydroxamic acid (SHAM) decreased the abundance of these DEGs. Interestingly, a high correlation presented between any two of saponin contents, key enzyme activities and expression levels of DEGs. Taken together, the inoculation of AMF can improve the growth and saponin accumulation of P. notoginseng by strengthening the activities of key enzymes and the expression levels of encoding genes, in which the JA regulatory pathway is a key link. This study provides references for implementing ecological planting of P. notoginseng, improving saponin accumulation and illustrating the biosynthesis mechanism.

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Section: Introductionmentioning

confidence: 99%

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Dai,

Zhang,

et al. 2024

Front. Plant Sci.

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“…The biosynthesis of triterpenoids in plants is associated with several biological processes, such as growth and development, defense responses, and interactions with environmental stimuli [13][14][15][16][17][18]. Studies have revealed that triterpenoids sourced from R. roxburghii possess multifaceted functionalities, ranging from treating depression and improving sleep, to managing enteritis, indicating their potential in various health domains [1,19,20].…”

Section: Introductionmentioning

confidence: 99%

Combined Metabolite and Transcriptomic Profiling Unveil a Potential Gene Network Involved in the Triterpenoid Metabolism of Rose roxburghii

Li,

Peng,

Yan

et al. 2024

IJMS

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Rose roxburghii, a horticulturally significant species within the Rosa genus of the Rosaceae family, is renowned for its abundance of secondary metabolites and ascorbate, earning it the title ‘king of vitamin C’. Despite this recognition, the mechanisms underlying the biosynthesis and regulation of triterpenoid compounds in R. roxburghii remain largely unresolved. In this study, we conducted high-performance liquid chromatography profiling across various organs of R. roxburghii, including fruit, root, stem, and leaves, revealing distinct distributions of triterpenoid compounds among different plant parts. Notably, the fruit exhibited the highest total triterpenoid content, followed by root and stem, with leaf containing the lowest levels, with leaf containing the lowest levels. Transcriptomic analysis unveiled preferential expression of members from the cytochrome P450 (CYP) and glycosyltransferase (UGT) families, likely contributing to the higher accumulation of both ascorbate and triterpenoid compounds in the fruits of R. roxburghii compared to other tissues of R. roxburghii. Transcriptomic analysis unveiled a potential gene network implicated in the biosynthesis of both ascorbate and triterpenoid compounds in R. roxburghii. These findings not only deepen our understanding of the metabolic pathways in this species but also have implications for the design of functional foods enriched with ascorbate and triterpenoids in R. roxburghii.

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“…Some studies prove that phytohormones regulate the seedling growth and fruit quality of R. roxburghii. For example, a low concentration (1 mM) of methyl jasmonate (MeJA) elicited the root growth of R. roxburghii and promoted triterpenoid synthesis [19]. Exogenous GA treatment was performed on the fruits of R. roxburghii in vitro [20].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Development in Rosa roxburghii

Huang,

Wu,

Sun

et al. 2023

Plants

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Gibberellins (GAs) play indispensable roles in the fruit development of horticultural plants. Unfortunately, the molecular basis behind GAs regulating fruit development in R. roxburghii remains obscure. Here, GA3 spraying to R. roxburghii ‘Guinong 5’ at full-bloom promoted fruit size and weight, prickle development, seed abortion, ascorbic acid accumulation, and reduction in total soluble sugar. RNA-Seq analysis was conducted to generate 45.75 Gb clean reads from GA3- and non-treated fruits at 120 days after pollination. We obtained 4275 unigenes belonging to differently expressed genes (DEGs). Gene ontology and the Kyoto Encyclopedia of Genes and Genomes displayed that carbon metabolism and oxidative phosphorylation were highly enriched. The increased critical genes of DEGs related to pentose phosphate, glycolysis/gluconeogenesis, and citrate cycle pathways might be essential for soluble sugar degradation. Analysis of DEGs implicated in ascorbate revealed the myoinositol pathway required to accumulate ascorbic acid. Finally, DEGs involved in endogenous phytohormones and transcription factors, including R2R3 MYB, bHLH, and WRKY, were determined. These findings indicated that GA3-trigged morphological alterations might be related to the primary metabolites, hormone signaling, and transcription factors, providing potential candidate genes that could be guided to enhance the fruit development of R. roxburghii in practical approaches.

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Low concentration of MeJA-elicited the positive growth of Rosa Roxburghii via balancing phytohormone signal transduction and triterpenoids synthesis

Cited by 4 publications

References 49 publications

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Improvement of Panax notoginseng saponin accumulation triggered by methyl jasmonate under arbuscular mycorrhizal fungi

Combined Metabolite and Transcriptomic Profiling Unveil a Potential Gene Network Involved in the Triterpenoid Metabolism of Rose roxburghii

Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Development in Rosa roxburghii

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