Low concentrations of Ambrosia maritima L. phenolic extract protect endothelial cells from oxidative cell death induced by H2O2 and sera from Crohn's disease patients

Ramli, Iman; Posadino, Anna Maria; Zerizer, Sakina; Spissu, Ylenia; Barberis, Antonio; Djeghim, Hanène; Emanuela, Azara; Bensouici, Chawki; Kabouche, Zahia; Rebbas, Khellaf; Droogmans, Guy; Sechi, Leonardo Antonio; Pintus, Gianfranco

doi:10.1016/j.jep.2022.115722

Cited by 11 publications

(6 citation statements)

References 76 publications

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“…Oxidative stress is a significant contributor to the pathophysiology of gastrointestinal mucosal illnesses, such as colitis, and is caused by shifts in the equilibrium between ROS generation and the capacity to quickly detoxify reactive intermediates [ 19 ]. Previous studies have also shown that CD patient sera have high ROS levels and have a pro-oxidative and cytotoxic effect on endothelial cells (ECs) cultured from CD patient sera, resulting in oxidative stress, CD serum-induced oxidative stress and ECs death [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology and molecular docking reveal the mechanism of Qinghua Xiaoyong Formula in Crohn’s disease

Fang

Pei

Peng

et al. 2023

Korean J Physiol Pharmacol

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Crohn's disease (CD) is a chronic inflammatory illness of the digestive system with unknown etiology, and its incidence is increasing worldwide. However, there are currently no effective treatments or medications available for individuals with CD. Therefore, novel therapeutic strategies are urgently needed. The bioactive compounds and targets associated with compounds of Qinghua Xiaoyong Formula (QHXYF) were examined using The Traditional Chinese Medicine Systems Pharmacology database, and 5 disease target databases were also used to identify CD-related disease targets. A total of 166 overlapping targets were identified from QHXYF-related and CD-related disease targets and they were found to be enriched in oxidative stress-related pathways and the PI3K/AKT signaling pathway. Molecular docking was then used to predict how the bioactive compounds would bind to the hub targets. It was found that quercetin could be the core bioactive compound and had good binding affinity to the top 5 hub targets. Finally, animal experiments were performed to further validate the findings, and the results revealed that QHXYF or quercetin inhibited 2,4,6-trinitrobenzenesulfonic acid-induced inflammation and oxidative stress processes by inhibiting the PI3K/AKT pathway, thereby improving CD symptoms. These findings suggest that QHXYF and quercetin may be potential novel treatments for CD.

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Section: Discussionmentioning

confidence: 99%

Network pharmacology and molecular docking reveal the mechanism of Qinghua Xiaoyong Formula in Crohn’s disease

Fang

Pei

Peng

et al. 2023

Korean J Physiol Pharmacol

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“…Unless differently stated, HKACs were cultured in 96-well black plates (Corning, Lowell, MA, USA) until 70 to 80% sub-confluence. According to previous experimentation using human sera [49][50][51], cells were serum-starved for 8 h before being treated for 12 h with POKF and POPBS at a final concentration of 5%. For the ROS and redox status evaluations (cytoplasmic and mitochondrial), cells were treated with H 2 O 2 at a 300 µM final concentration for 3 h, without removing the serum treatment.…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Protective Effect of Knee Postoperative Fluid on Oxidative-Induced Damage in Human Knee Articular Chondrocytes

Giordo,

Tulasigeri Totiger,

Caggiari

et al. 2024

Antioxidants

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The oxidative-stress-elicited deterioration of chondrocyte function is the initial stage of changes leading to the disruption of cartilage homeostasis. These changes entail a series of catabolic damages mediated by proinflammatory cytokines, MMPs, and aggrecanases, which increase ROS generation. Such uncontrolled ROS production, inadequately balanced by the cellular antioxidant capacity, eventually contributes to the development and progression of chondropathies. Several pieces of evidence show that different growth factors, single or combined, as well as anti-inflammatory cytokines and chemokines, can stimulate chondrogenesis and improve cartilage repair and regeneration. In this view, hypothesizing a potential growth-factor-associated action, we investigate the possible protective effect of post-operation knee fluid from patients undergoing prosthesis replacement surgery against ROS-induced damage on normal human knee articular chondrocytes (HKACs). To this end, HKACs were pre-treated with post-operation knee fluid and then exposed to H2O2 to mimic oxidative stress. Intracellular ROS levels were measured by using the molecular probe H2DCFDA; cytosolic and mitochondrial oxidative status were assessed by using HKACs infected with lentiviral particles harboring the redox-sensing green fluorescent protein (roGFP); and cell proliferation was determined by measuring the rate of DNA synthesis with BrdU incorporation. Moreover, superoxide dismutase (SOD), catalase, and glutathione levels from the cell lysates of treated cells were also measured. Postoperative peripheral blood sera from the same patients were used as controls. Our study shows that post-operation knee fluid can counteract H2O2-elicited oxidative stress by decreasing the intracellular ROS levels, preserving the cytosolic and mitochondrial redox status, maintaining the proliferation of oxidatively stressed HKACs, and upregulating chondrocyte antioxidant defense. Overall, our results support and propose an important effect of post-operation knee fluid substances in maintaining HKAC function by mediating cell antioxidative system upregulation and protecting cells from oxidative stress.

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“…Ambrosia maritima L., traditionally known as "Damsissa" in Sudan is widely used in North and Central Sudan in folk medicine to treat respiratory problems, diabetes and hypertension [1]. This plant are used in North African folk medicine to treat infections, inflammatory diseases, gastrointestinal and urinary tract disturbances, rheumatic pain, diabetes and cancer [2].…”

Section: Introductionmentioning

confidence: 99%

Untitled

2024

NSJ

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In this study, Gas Chromatography-Mass Spectrometry (GC-MS) was used to compare the chemical compositions of Ambrosia maritima aerial parts collected from two different locations in Ed-Damer region, River Nile State, Northern Sudan. The results showed that 14 compounds of the two plant samples contained the same chemical composition, which was 2-methoxy-4-vinyl phenol, Decanoic acid ethyl ether, 1-cyclohexene 1-ol, 2,6dimethyl acetate, 2-acetoxy 1,1,10 trimethyl 6,9-epidioxydecalin, 6,8-nonadien-2-one, 6methyl-5-(1-methylethylidene-, Ethyl alpha-d-glucopyranoside, Hexadecanoic acid, methyl ester, n-Hexadecanoic acid, Hexadecanoic acid, ethyl ester, Phytol, Androstan-17one,3-ethyl-3-hydroxy (5-alpha), 17-beta-hydroxy-6-oxo-4,5-secoandrosta-, 5-alpha-17alpha pregnan-12 one and Cyclohexanamine,N-(hexa-hydro-3 methylene 2(3H) -Comp. of Chem. Composition of A. maritima Elshafie et. al.benzofuranyledene)-but the percentage content was different. The main components of the ethanolic extracts of the two samples were different. N,N-dimethylglycine (2.16%) , Norethindrone (19.76%), alpha-amyrin (0.66%) and others were only detected in Ambrosia maritima (sample Am1), Nitroacetamine (16.07%) , linoleic acid, ethyl ester (9.83%), gamma-sitosterol (5.59%), and others were only detected in Ambrosia maritima (sample Am2).

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Low concentrations of Ambrosia maritima L. phenolic extract protect endothelial cells from oxidative cell death induced by H2O2 and sera from Crohn's disease patients

Cited by 11 publications

References 76 publications

Network pharmacology and molecular docking reveal the mechanism of Qinghua Xiaoyong Formula in Crohn’s disease

Network pharmacology and molecular docking reveal the mechanism of Qinghua Xiaoyong Formula in Crohn’s disease

Protective Effect of Knee Postoperative Fluid on Oxidative-Induced Damage in Human Knee Articular Chondrocytes

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