Low concentrations of caffeine induce asymmetric cell division as observed in vitro by means of the CBMN-assay and iFISH

Hatzi, Vasiliki I.; Karakosta, Maria; Barszczewska, Katarzyna; Karachristou, Ioanna; Pantelias, Gabriel E.; Terzoudi, Georgia I.

doi:10.1016/j.mrgentox.2015.08.002

Cited by 12 publications

(10 citation statements)

References 48 publications

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“…For example, caffeine-induced DNA damage and checkpoint abrogation misaligns chromosomes and induces asymmetric cell division in lymphocytes [33] while chromosome misalignment is also able to induce spindle-positioning defects [12]. Spindle-pole derived signals are critical to spindle positioning [10]; therefore, the induction of multipolar spindles through centrosome amplification would be predicted to disturb these positioning gradients.…”

Section: Discussionmentioning

confidence: 99%

“…Given the multifunctional nature of BRCA1 and its potential to impact many critical nodes in the spindle positioning pathway, it is possible that the randomization of the cell division axis may result through the suppression of other BRCA1 functions, such as DNA damage repair [ 30 ], the NEAT1/ miRNA axis [ 31 ], or the RANK/ RANKL signaling pathway [ 32 ]. For example, caffeine-induced DNA damage and checkpoint abrogation misaligns chromosomes and induces asymmetric cell division in lymphocytes [ 33 ] while chromosome misalignment is also able to induce spindle-positioning defects [ 12 ]. Spindle-pole derived signals are critical to spindle positioning [ 10 ]; therefore, the induction of multipolar spindles through centrosome amplification would be predicted to disturb these positioning gradients.…”

Section: Discussionmentioning

confidence: 99%

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BRCA1 controls the cell division axis and governs ploidy and phenotype in human mammary cells

Kannan

Nemirovsky

et al. 2017

Oncotarget

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BRCA1 deficiency may perturb the differentiation hierarchy present in the normal mammary gland and is associated with the genesis of breast cancers that are genomically unstable and typically display a basal-like transcriptome. Oriented cell division is a mechanism known to regulate cell fates and to restrict tumor formation. We now show that the cell division axis is altered following shRNA-mediated BRCA1 depletion in immortalized but non-tumorigenic, or freshly isolated normal human mammary cells with graded consequences in progeny cells that include aneuploidy, perturbation of cell polarity in spheroid cultures, and a selective loss of cells with luminal features. BRCA1 depletion stabilizes HMMR abundance and disrupts cortical asymmetry of NUMA-dynein complexes in dividing cells such that polarity cues provided by cell-matrix adhesions were not able to orient division. We also show that immortalized mammary cells carrying a mutant BRCA1 allele (BRCA1 185delAG/+) reproduce many of these effects but in this model, oriented divisions were maintained through cues provided by CDH1+ cell-cell junctions. These findings reveal a previously unknown effect of BRCA1 suppression on mechanisms that regulate the cell division axis in proliferating, non-transformed human mammary epithelial cells and consequent downstream effects on the mitotic integrity and phenotype control of their progeny.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

BRCA1 controls the cell division axis and governs ploidy and phenotype in human mammary cells

Kannan

Nemirovsky

et al. 2017

Oncotarget

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“…Wizualizacja mikrojąder oparta jest o barwienie przyżyciowe komórek za pomocą barwników fluorescencyjnych, np. oranż akrydyny lub Hoechst 33258, lub niefluorescencyjnych (barwnik Giemsy) [38,39].…”

Section: Test Mikrojądrowy In Vitrobiomarker Skutków Narażenia Organiunclassified

The application of in vitro models in a preclinical safety evaluation of new drug candidates

Klaś¹,

Guzy²,

Piska³

et al. 2018

Farm Pol.

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Hodowle komórkowe in vitro to powszechnie wykorzystywany model do określania cytotoksyczności potencjalnych leków, pestycydów czy innych The application of in vitro models in a preclinical safety evaluation of new drug candidates • In vitro models are an essential tool to determine the profile of activity and the safety of the drug candidate at the early stage of preclinical studies. Thanks to them it is possible to perform screening tests for thousands of active molecules in a short time and to predict their possible side effect. This allows to eliminate potentially dangerous compounds at a very early stage, before using animal models. This review presents the most important information about using in vitro methods, based on prokaryotic and eukaryotic cell models, in hepato-, neuro-, nephro-, cardio-and genotoxicity research of active compounds.

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“…Indeed, Bakker and associates 2010 reported that pregnant women who consumed the caffeine equivalent of six or more cups of coffee were more likely to give birth to babies who were small for gestational age (29). Hatzi and associates 2015 demonstrated that caffeine could induce asymmetric cell division of peripheral lymphocytes in vitro (30). The errors in chromosome distribution that can occur with asymmetric divisions could impair the viability of a pregnancy (30).…”

Section: Biological Mechanisms: Pregnancymentioning

confidence: 99%

“…Hatzi and associates 2015 demonstrated that caffeine could induce asymmetric cell division of peripheral lymphocytes in vitro (30). The errors in chromosome distribution that can occur with asymmetric divisions could impair the viability of a pregnancy (30).…”

Section: Biological Mechanisms: Pregnancymentioning

confidence: 99%

The effect of caffeine on fecundability : differences in coffee, tea, and cola consumption.

Steinmetz¹

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This study examined whether intake of caffeinated beverages (coffee, tea, and cola) was associated with fecundability (time to pregnancy) in a prospective cohort study. Data from 470 women from the Mount Sinai Study of Women Office Workers (1990-1994) were analyzed. Intake of coffee, tea, cola, and other variables were recorded in daily diaries and calculated as menstrual cycle level means for up to 20 cycles. Pregnancy was assayed using hCG and confirmed by physician diagnosis. The associations of caffeinated beverages with the probability of becoming pregnant during a given cycle were determined using discrete survival analysis, adjusted for potential confounders. Overall caffeine intake was not significantly associated with fecundability. Moderate intake of coffee, tea and cola was associated with increased fecundability, and high intake with decreased, though most associations were not statistically significant. Moderation in caffeinated beverage consumption appears to be important for women who are trying to conceive. vi

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Low concentrations of caffeine induce asymmetric cell division as observed in vitro by means of the CBMN-assay and iFISH

Cited by 12 publications

References 48 publications

BRCA1 controls the cell division axis and governs ploidy and phenotype in human mammary cells

BRCA1 controls the cell division axis and governs ploidy and phenotype in human mammary cells

The application of in vitro models in a preclinical safety evaluation of new drug candidates

The effect of caffeine on fecundability : differences in coffee, tea, and cola consumption.

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