Low-Cost and Convenient Microchannel Resistance Biosensing Platform by Directly Translating Biorecognition into a Current Signal

He, Huiyu; Nie, Rongbin; Peng, Lu; Peng, Xuewen; Li, Xiaohan

doi:10.1021/acs.analchem.1c03006

Cited by 12 publications

(4 citation statements)

References 38 publications

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“…By monitoring the changes in the current at both ends of the microchannel (Scheme 1c), we can transfer the antigen−antibody recognition signal into the target concentration and quantitatively analyze the target (Scheme 1d). In LF-EMB, the one-button current-monitoring device (OCD), introduced in our previous studies, 21 and the microfluid chip were designed to form an all-in-one signal readout device (Figure S1). For the narrow space in the microchannel, the efficiency of antigen−antibody recognition was higher due to the intense Brownian motion among all biomolecules and is suitable for detecting targets, which is valuable and challenging to achieve.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Microchannel Immunosensor Based on Antibody–Antigen Biorecognition-Induced Charge Quenching

et al. 2022

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Pursuing convenient operations and precise testing have become an urgent requirement in clinical diagnosis, treatment, and prognosis. Label-free detection is desirable for obviating the labeling process while maintaining high sensitivity and efficiency. Here, we used the dual properties of highly selective antibody−antigen recognition and potential signaling of biomolecules to construct a label-free electroosmotic flow-driven microchannel (LF-EMB) biosensor based on an antibody−antigen biorecognition-induced charge quenching theory proposed herein. The LF-EMB consists of a one-step immune-reaction, onebutton portable device, and supporting microfluidic chip, providing a highpowered tool for rapid on-site testing. The LF-EMB quantified interleukin-6 and kanamycin levels down to 1 pg/mL and 5 pg/mL, respectively, with the whole analysis completed within 35 min. The outstanding sensitivity and detection speed of the constructed LF-EMB provide a convenient option for the quantitative detection of inflammatory markers and antibiotics.

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Section: Introductionmentioning

confidence: 99%

Label-Free Microchannel Immunosensor Based on Antibody–Antigen Biorecognition-Induced Charge Quenching

et al. 2022

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“…Fundamental electrical signals such as current, resistance, and voltage, which are robust against external disturbances, can be efficiently captured with an affordable, compact multimeter. 15 Tang et al converted the gas pressure into a resistance signal and used a digital multimeter as readout to realize a simple and portable detection of carcinoembryonic antigen. 16 Continued focus and innovation are essential to advance new POCT methodologies.…”

mentioning

confidence: 99%

“…Such signals can be detected with conventional equipment, making them suitable for the construction of portable and miniaturized analytical sensors. Fundamental electrical signals such as current, resistance, and voltage, which are robust against external disturbances, can be efficiently captured with an affordable, compact multimeter . Tang et al converted the gas pressure into a resistance signal and used a digital multimeter as readout to realize a simple and portable detection of carcinoembryonic antigen .…”

mentioning

confidence: 99%

Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout

Cai,

Huang,

Lin

et al. 2024

ACS Sens.

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Changes in the charge density on the inner surface of the microchannel can modulate the ion concentration at the tip, thus causing changes in the resistance of the system. In this study, this property is adopted to construct a portable sensor using a multimeter and aflatoxin B1 (AFB1) is used as the model target. Initially, the cDNA/aptamer complex is modified in the microchannel. The inner microchannel surface's charge density is then altered by the recognition of the target, leading to a change in the system's resistance, which can be conveniently monitored using a multimeter. Critical parameters influencing the performance of the system are optimized. Under optimum conditions, the resistance is linearly related to the logarithm of AFB1 concentration in the range of 100 fM−10 nM and the detection limit is 46 fM (S/N = 3). The resistive measurement is separated from the recognition reaction of the target, reducing the matrix interference during the detection process. This sensor boasts high sensitivity and specificity coupled with commendable reproducibility and stability. It is applied to assay the AFB1 content successfully in an actual sample of corn. Moreover, this approach is cost-effective, user-friendly, and highly accurate.

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“…This can be particularly challenging when conducting tests in remote, resource-poor rural areas and field environments, where inadequate infrastructure frequently presents the greatest hindrance to environmental monitoring. Generally, conventional methods of pollutant detection rely on expensive laboratory equipment and specialized expertise, including sample collection and laboratory analysis using techniques, such as polymerase chain reaction (PCR), label-free electrical immunosensor, biosensing, − mass spectrometry, and so on. − Despite these methods showing promising accuracy and sensitivity, the need for developing robust and portable sensing strategies with high sensitivity remains imperative, particularly for big data analysis in low-resource rural areas. Visual inspection platforms, including infiltrated visual detection, optical detection, and portable instrument-based detection, have been deemed promising portable detection strategies. − These platforms provide easily and intuitively readable detection signals without the need for complex and time-consuming laboratory instrument analysis. , Among visual inspection platforms, wettability-based portable sensing systems are gaining increasing attention due to their ultrasensitivity, low cost, ease of use, and fast sensing procedure.…”

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confidence: 99%

Nano-Structural Superwetting Surfaces for Highly Reliable On-Site Detection of Bisphenol A

Dai,

Liu,

Zhu

et al. 2023

Anal. Chem.

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In the domain of big data geographic screening for environmental pollutants, the expeditious dissemination of testing results to environmental investigation professionals is pivotal in facilitating comprehensive analysis and the implementation of more efficacious strategies for managing environmental issues. However, this endeavor can prove to be particularly arduous when conducting examinations in remote, resource-scarce rural areas and field environments, where deficient infrastructure often emerges as the principal impediment to unimpeded environmental monitoring. Therefore, the development of a reliable and portable monitoring strategy with the ability to analyze large amounts of data is highly required. Here, a deep-learning (DL)-assisted portable sensing strategy was developed based on thermal and pH dual-responsive nano-structural superwetting surfaces, for highly reliable, quick, and field monitoring of environmental pollutants. In our experiment, bisphenol A (BPA) was selected as the representative pollute. The achieved limit of detection, attaining a remarkably low value of 1.05 μM, unequivocally adhered to stringent international testing standards for evaluating the migration of BPA in thermal paper. Based on a DL image classification algorithm, highly precise predictions regarding the migration of BPA concentration were achieved, with an accuracy rate exceeding 99%. Furthermore, it successfully facilitated automated and exceedingly reliable monitoring of the migration of BPA from thermal paper within the principal provinces of thermal paper production in China. This strategy engenders the potential to establish correlations between environmental pollutant concentrations in specific regions and the prevalence of certain human ailments.

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Low-Cost and Convenient Microchannel Resistance Biosensing Platform by Directly Translating Biorecognition into a Current Signal

Cited by 12 publications

References 38 publications

Label-Free Microchannel Immunosensor Based on Antibody–Antigen Biorecognition-Induced Charge Quenching

Label-Free Microchannel Immunosensor Based on Antibody–Antigen Biorecognition-Induced Charge Quenching

Portable Sensor for Aflatoxin B1 Based on the Regulation of Resistance of a Microchannel Using a Multimeter as Readout

Nano-Structural Superwetting Surfaces for Highly Reliable On-Site Detection of Bisphenol A

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