Determining pre-harvest fruit maturity is vital to ensure the quality of kiwifruit, and dry-matter content is an important indicator of kiwifruit ripeness. To predict the pre-harvest dry-matter content of kiwifruit continuously in real-time with high accuracy, this study uses hyperspectral data of pre-harvest Jintao kiwifruit obtained by using a hyperspectral image acquisition device. The raw data underwent whiteboard correction, spectral data extraction, spectral pre-processing, and feature-band extraction, following which the dry-matter content of the fruit was predicted by using partial least squares (PLS) regression. The feature bands extracted by the random frog method were 538.93, 671.14, 693.41, 770.61, 796.98, 813.24, 841.21, 843.29, and 856.80 nm, which improve the accuracy of the PLS method for predicting dry-matter content, with R2 = 0.92 and a root mean square error (RMSE) of 0.41% for the training set, and R2 = 0.85 and a RMSE of 0.50% for the test set. These results show that the proposed method reduces the number of required bands while maintaining the prediction accuracy, thereby demonstrating the reliability of using hyperspectral data to predict the pre-harvest dry-matter content of kiwifruit. This method can effectively guide the management of kiwifruit harvesting period, establishing a theoretical foundation for precise unmanned harvesting.