Low-cost assays in paper-based microfluidic biomedical devices

Benhabib, Merwan; Li, XiuJun J.

doi:10.1533/9780857097040.4.492

Cited by 9 publications

(4 citation statements)

References 71 publications

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“…Microfluidic paper-based analytical devices (μPADs) are one particular type of POC devices first developed by Martinez et al, which represent an attractive solution due to their portability, versatility, ease of use, and low cost. Wax printing is one of the several techniques (in addition to inkjet and screen printing , among others) that can be used to create hydrophobic channels on paper substrates. , The wax and the substrate can also be functionalized, allowing the development of μPADs with multifunctional properties that can add and/or improve the testing and analytical capabilities. – Wax printing has been used to evaluate proofs of concept for POC devices that are able to carry out colorimetric assays, where a color change in response to a chemical reaction signals the presence or quantity of a specific analyte . Further, research has also been focusing on the development of alternative microfluidic substrates to paper, allowing to introduce additional features, including electroactivity and reusability in poly(vinylidene- co -trifluoroethylene) and poly( l -lactic acid) or degradability in poly( d , l -lactic- co -glycolic acid) .…”

Section: Introductionmentioning

confidence: 99%

Natural Indigenous Paper Substrates for Colorimetric Bioassays in Portable Analytical Systems: Sustainable Solutions from the Rain Forests to the Great Plains

Brito-Pereira,

Silva Macedo,

Ribeiro

et al. 2023

ACS Appl. Mater. Interfaces

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Point-of-care (POC) devices can provide inexpensive, practical, and expedited solutions for applications ranging from biomedicine to environmental monitoring. This work reports on the development of low-cost microfluidic substrates for POC systems suitable for analytical assays, while also satisfying the need for social and environmentally conscious practices regarding circular economy, waste reduction, and the use of local resources. Thus, an innovative greener process to extract cellulose from plants including abaca, cotton, kozo, linen, and sisal, originating from different places around the world, is developed, and then the corresponding paper substrates are obtained to serve as platforms for POC assays. Hydrophobic wax is used to delineate channels that are able to guide solutions into chambers where the colorimetric assay for total cholesterol quantification is carried out as a proof of concept. Morphological and physicochemical analyses are performed, including the evaluation of fiber diameter, shape and density, and mechanical and thermal properties, together with peel adhesion of the printed wax channels. Contact angle and capillary flow tests ascertain the suitability of the substrates for liquid assays and overall viability as low-cost, sustainable microfluidic substrates for POC applications.

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Section: Introductionmentioning

confidence: 99%

Natural Indigenous Paper Substrates for Colorimetric Bioassays in Portable Analytical Systems: Sustainable Solutions from the Rain Forests to the Great Plains

Brito-Pereira,

Silva Macedo,

Ribeiro

et al. 2023

ACS Appl. Mater. Interfaces

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“…To address these issues, we adopted the microfluidic chip technology, which has greatly advanced in past few decades, enabling researchers to study many disease mechanisms [14][15][16][17][18] . The microfluidic chip has been simulated, designed, and tested for studying polymeric particles and biological cells [19][20][21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

Microfluidic chip enables single-cell measurement for multidrug resistance in triple-negative breast cancer cells

Parekh¹,

Noghabi²,

López³

et al. 2020

CDR

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Aims: Triple-negative breast cancer patients are commonly treated with combination chemotherapy. Nonetheless, outcomes remain substandard with relapses being of a frequent occurrence. Among the several mechanisms that result in treatment failure, multidrug resistance, which is mediated by ATP-binding cassette proteins, is the most common. Regardless of the substantial studies conducted on the heterogeneity of cancer types, only a few assays can distinguish the variability in multidrug resistance activity between individual cells. We aim to develop a single-cell assay to study this. Methods: This experiment utilized a microfluidic chip to measure the drug accumulation in single breast cancer cells in order to understand the inhibition of drug efflux properties. Results: Selection of single cells, loading of drugs, and fluorescence measurement for intracellular drug accumulation were all conducted on a microfluidic chip. As a result, measurements of the accumulation of chemotherapeutic drugs (e.g., daunorubicin and paclitaxel) in single cells in the presence and absence of cyclosporine A were conducted. Parameters such as initial drug accumulation, signal saturation time, and fold-increase of drug with and without the presence cyclosporine A were also tested. Conclusion: The results display that drug accumulation in a single-cell greatly enhanced over its same-cell control because of inhibition by cyclosporine A. Furthermore, this experiment may provide a platform for future liquid biopsy studies to characterize the multidrug resistance activity at a single-cell level.

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“…There are several types of paper that can be used as a substrate for µPADs. The criteria for paper selection depends on the application and fabrication method of the devices [46]. Cellulose paper such as Whatman® filter is commonly used as a substrate for µPADs because it is cheap and hydrophilic which allows the penetration of fluid.…”

Section: Types Of Paper For µPadsmentioning

confidence: 99%

“…Cellulose paper such as Whatman® filter is commonly used as a substrate for µPADs because it is cheap and hydrophilic which allows the penetration of fluid. Whatman No.1 filter paper is most intensively used because of the medium retention and flow rate of fluids and its compatibility with many fabrication methods [46]. Some applications or fabrication methods which require solvents that swell the cellulose fiber and restrict the pore sizes need Whatman No.4 filter paper as a substrate because it has larger pore sizes, resulting in larger surface area and faster flow rate [47].…”

Section: Types Of Paper For µPadsmentioning

confidence: 99%

Development of microfluidic paper-based analytical device for determination of creatinine in urine samples

Sununta

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The simple, low-cost and portable microfluidic paper-based analytical devices (µPADs) for colorimetric determination of creatinine in urine samples were developed. The methodology was based on Jaffé reaction between the creatinine and picric acid in alkaline medium generating a colorimetric creatinine-alkaline picrate complex. The product exhibits an orange color which is clearly visible on µPADs. The color intensity of the complex which is indicative of the concentration of creatinine is then quantitatively determined using ImageJ software. Various experimental parameters were optimized, i.e., concentration of picric acid, concentration of sodium hydroxide, and reaction time, and the best analytical performance was achieved with 0.04 M picric acid, 2 M sodium hydroxide, and 25 minutes of reaction time. Under the optimum conditions, a wide linear range was obtained in the range of 0.2 – 1 mM with a limit of detection (LOD) and a limit of quantitation (LOQ) of 0.08 mM and 0.26 mM, respectively. Moreover, this method provided a selective determination of creatinine and showed a good reproducibility with a precision of 2.85, 1.10 and 1.64 %RSD for the detection of creatinine at 0.2, 0.6 and 1 mM, respectively. The accuracy of the proposed method was not significantly different from the standard Jaffé method by a paired t-test at 95% confidence interval. Finally, the developed devices were successfully applied for the determination of creatinine in urine samples with %recoveries in the range of 91.06-102.86%.

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Low-cost assays in paper-based microfluidic biomedical devices

Cited by 9 publications

References 71 publications

Natural Indigenous Paper Substrates for Colorimetric Bioassays in Portable Analytical Systems: Sustainable Solutions from the Rain Forests to the Great Plains

Natural Indigenous Paper Substrates for Colorimetric Bioassays in Portable Analytical Systems: Sustainable Solutions from the Rain Forests to the Great Plains

Microfluidic chip enables single-cell measurement for multidrug resistance in triple-negative breast cancer cells

Development of microfluidic paper-based analytical device for determination of creatinine in urine samples

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