Low-cost biotransformation of glycerol to 1,3-dihydroxyacetone throughGluconobacter frateuriiin medium with inorganic salts only

Abstract

This study illustrates the advantages of inorganic nutrients supplementation over organic nutrient supplementation for a lower media cost and a higher dihydroxyacetone (DHA) production yield through Gluconobacter frateurii BCC 36199 cultivation. The study found that the use of media that contain only glycerol and inorganic salts enhanced DHA production (DHA-Prod) while keeping the production of bacterial biomass at a sufficient level. Most of the starting material, that is, glycerol, is converted into DHA, which is the target of the production process. The cost of the nitrogen supplement in the DHA-Prod process may be reduced by up to 80% through the use of the inorganic culture medium that has been developed in this study.

Effect of NaCl removal from biodiesel‐derived crude glycerol by ion exchange to enhance dihydroxyacetone production by Gluconobacter thailandicus in minimal medium

“…Table shows that every 1 kg of refined glycerol can produce 0.97 kg of DHA ( y sp = 99%). Given that the price of DHA is 25 USD kg −1 , this results in a profit of 23.01 USD per 1 kg of glycerol.…”

“…The production cost can be decreased by using minimal media containing glycerol, supplemented with a much less expensive inorganic nutrient source. It has been reported that minimal media containing only glycerol and inorganic salts could achieve 96.77% DHA production yields ( y sp ) (DHA moles per glycerol moles) . In addition to the use of an inorganic nitrogen and phosphorous source, this study uses crude glycerol to further minimize the cost of production.…”

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 10: Suitability of taxonomic units notified to EFSA until March 2019

“…There are only a limited number of articles published on this TU. It can produce high amounts of fructans and can efficiently produce glyceric acid from raw glycerol (Poljungreed and Boonyarattanakalin, ). It is an obligate aerobic acetic acid bacterium that is found as a spontaneous contaminant as part of the microbial community in some food processes (e.g.…”

Sustainability Assessment of Dihydroxyacetone (DHA) Production from Glycerol: A Comparative Study between Biological and Catalytic Oxidation Routes

“…32 Among these, Gluconobacter strains have been used extensively in recent studies of DHA production as shown in Table 1. 23,32,34,35 Although the biological route can give excellent DHA selectivity, it has some limitations. The main challenge of DHA production is the inhibitory effect on bacterial growth due to the high concentrations of glycerol, 5,36 leading to the low DHA production capacity.…”

“…Commercial synthesis of DHA is preferred via the biological route as compared to the chemical route due to the nature of their microorganisms and the mild process environment. , The industrial strains for producing DHA from glycerol are Acetobacter species, Yeast species, and Gluconobacter species . Among these, Gluconobacter strains have been used extensively in recent studies of DHA production as shown in Table . ,,, Although the biological route can give excellent DHA selectivity, it has some limitations. The main challenge of DHA production is the inhibitory effect on bacterial growth due to the high concentrations of glycerol, , leading to the low DHA production capacity.…”