Low cost fabrication of microfluidic paper-based analytical devices with water-based polyurethane acrylate and their application for bacterial detection

Abstract

This study presents a simple, inexpensive and environment-friendly fabrication strategy for microfluidic paperbased analytical devices which can resist the penetration of surfactant solutions and organic solvents, by using water-based polyurethane acrylate via UV light curing. The filter paper's barrier created using cured PUA could withstand surfactant solutions (10 wt%, CTAB, SDS and Triton X-100) and organic solvents (methanol, isopropanol, DMF, DMSO, etc). This is very useful for analyzing complicated biological samples on the microfluidic paper-based analytical devices. In addition, the expense of water-based polyurethane acrylate is very cheap (about $8/500 g) and PUA developer is water that is environmental-friendly. To further verify its advantage, we successfully demonstrated the proposed microfluidic devices for detection of E. coli targets in tap water and seawater via colorimetric analysis in a fast and convenient manner. Our results revealed that the linear response to E. coli BL21 was in the range of 10 4 ∼10 9 cfu/mL. The proposed method can effectively avoid the damage for the hydrophobic barriers from the solution even some aggressive liquids, and shows great potential in on-site analysis, environmental monitoring, and food safety.

Paper-based PCR method development, validation and application for microbial detection

“…The bacteria in water samples are pre-concentrated using antibody-coated immune-magnetic nanoparticles and then tested with the paper-based microfluidic device. Similarly, Lin et al [ 22 ] recently showed in their study that a PUA cured paper-based device can potentially detect microbes present in some aggressive liquids. Molina et al [ 23 ] used a different strategy for differential identification of bacteria with the aid of multiplex PCR.…”

Microfluidics for core–shell drug carrier particles – a review

“…[18][19][20] PDMS is the most common material used to fabricate microuidic devices because of the ease of fabrication, optical transparency, gas permeability, low chemical reactivity, and inexpensiveness. 17,21,22 It is also used in bioassays and targeted drug delivery systems (DDS) using implantable microuidic devices. 23 The microstructure is generally fabricated by so lithography.…”

Fabrication of paper microfluidic devices using a toner laser printer

“…1,[29][30][31][32][33] These mPADs were used to perform analytical assays for point-of-care diagnostics and environmental monitoring. 2,18,19,28,[34][35][36] Such methods of fabrication required particular materials (synthetic hydrophobic precursor [37][38][39] and photoresist 1,32 ) and equipment (wax printer, 20,21,[40][41][42][43][44][45] inkjet printer, 22,23 laser, 39,45 mask aligner, 1 and UV lamp 32,33,37,38 ) to create the devices. Fabrication of mPAD has been initially demonstrated via photolithography, while direct printing of hydrophobic materials has been developed as alternative routes.…”