Low Cost Medium for Spore Production of Bacillus KKU02 and KKU03 
 and the Effects of the Produced Spores on Growth of Giant Freshwater Prawn (Macrobrachium 
 rosenbergii de Man)

Wangka-orm, Charkrit; DEESEENTHUM, SIRIRAT; Leelavatcharamas, Vichai

doi:10.3923/pjbs.2014.1015.1022

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…Application of spore utilization as probiotics has been studied which provides beneficial effects on cultivated organism such as shrimp and fish (Wangka-Orm et al, 2014;Thurlow et al, 2019). One of the bacteria from the genus Bacillus that can produce spores is B. megaterium which presents its own uniqueness in its outer membrane known as exosporium (Zhou et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The Influence of Environmental Shock Initiation on Vegetative and Spore Production of Bacillus megaterium

Mahariawan¹,

Kusuma²,

Yuniarti³

et al. 2022

EEC

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Production of B. megaterium spores as probiotic candidates is not only influenced by the nutrient composition in the culture media but also initiated by environmental factors. One of the efforts to reduce spore production costs is by utilizing wheat flour as a medium for spore growth. Large quantities of B. megaterium spore production in a short time on wheat flour media can be carried out by implementing environmental shocks. The objective of this study was to evaluate the influence of environmental shock initiation on the spore production of B. megaterium. The culture media consisted of carbon source (wheat flour) and nitrogen (ammonium chloride) at the C/N ratio of 15:3. Environmental shock conducted at 51 Â°C and pH of 10 in vegetative maintenance. The treatments of shock were started at the 5th, 10th, and 15th hour after inoculation, respectively. The parameters of this study were vegetative cells, spore production, sporulation efficiency and spore fraction. The highest vegetative cell was revealed in the shock initiated at 10th hour (3.5 x 108 cells. ml-1). The high density of vegetative cells did not indicate high spore production. The highest spore production in this study was found in the shock started at 15th hour (1.73 x 108 spores. ml-1) with sporulation efficiency (79%) and spore fraction was 57.28%. The target of high spore production can be achieved in the shock initiation at the 15th hour in wheat flour media.

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Section: Introductionmentioning

confidence: 99%

The Influence of Environmental Shock Initiation on Vegetative and Spore Production of Bacillus megaterium

Mahariawan¹,

Kusuma²,

Yuniarti³

et al. 2022

EEC

View full text Add to dashboard Cite

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Elucidation of Bacillus subtilis KATMIRA 1933 Potential for Spore Production in Submerged Fermentation of Plant Raw Materials

Khardziani

Kachlishvili

Sokhadze

et al. 2017

Probiotics & Antimicro. Prot.

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In this study, the effects of several key factors to increase spore production by Bacillus subtilis subsp. KATMIRA 1933 were evaluated in shake flask experiments. In a synthetic medium, glucose concentration played a crucial role in the expression of bacilli sporulation capacity. In particular, maximum spore yield (2.3 × 10 spores/mL) was achieved at low glucose concentration (2 g/L), and further gradual increase of the carbon source content in the medium caused a decrease in sporulation capacity. Substitution of glucose with several inexpensive lignocellulosic materials was found to be a reasonable way to achieve high cell density and sporulation. Of the materials tested, milled mandarin peels at a concentration of 40 g/L served as the best growth substrate. In these conditions, bacilli secreted sufficient levels of glycosyl hydrolases, providing slow hydrolysis of the mandarin peel's polysaccharides to metabolizable sugars, providing the bacterial culture with an adequate carbon and energy source. Among nitrogen sources tested, peptone was found to favor spore production. Moreover, it was shown that cheese and cottage cheese whey usage, instead of distilled water, significantly increases spore formation. After optimization of the nutrient medium in the shake flask experiments, the technical feasibility of large-scale spore production by B. subtilis KATMIRA 1933 was confirmed in a laboratory fermenter. The spore yield (7 × 10 spores/mL) obtained using a bioreactor was higher than those previously reported.

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Bacillus amyloliquefaciens Spore Production Under Solid-State Fermentation of Lignocellulosic Residues

Berikashvili

Sokhadze

Kachlishvili

et al. 2017

Probiotics & Antimicro. Prot.

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This study was conducted to elucidate cultivation conditions determining Bacillus amyloliquefaciens B-1895 growth and enhanced spore formation during the solid-state fermentation (SSF) of agro-industrial lignocellulosic biomasses. Among the tested growth substrates, corncobs provided the highest yield of spores (47 × 10 spores g biomass) while the mushroom spent substrate and sunflower oil mill appeared to be poor growth substrates for spore formation. Maximum spore yield (82 × 10 spores g biomass) was achieved when 15 g corncobs were moistened with 60 ml of the optimized nutrient medium containing 10 g peptone, 2 g KHPO, 1 g MgSO·7HO, and 1 g NaCl per 1 l of distilled water. The cheese whey usage for wetting of lignocellulosic substrate instead water promoted spore formation and increased the spore number to 105 × 10 spores g. Addition to the cheese whey of optimized medium components favored sporulation process. The feasibility of developed medium and strategy was shown in scaled up SSF of corncobs in polypropylene bags since yield of 10 × 10 spores per gram of dry biomass was achieved. In the SSF of lignocellulose, B. amyloliquefaciens B-1895 secreted comparatively high cellulase and xylanase activities to ensure good growth of the bacterial culture.

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Low Cost Medium for Spore Production of Bacillus KKU02 and KKU03 and the Effects of the Produced Spores on Growth of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)

Cited by 5 publications

References 21 publications

The Influence of Environmental Shock Initiation on Vegetative and Spore Production of Bacillus megaterium

The Influence of Environmental Shock Initiation on Vegetative and Spore Production of Bacillus megaterium

Elucidation of Bacillus subtilis KATMIRA 1933 Potential for Spore Production in Submerged Fermentation of Plant Raw Materials

Bacillus amyloliquefaciens Spore Production Under Solid-State Fermentation of Lignocellulosic Residues

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