Low cost micro-Coulter counter with hydrodynamic focusing

Rodriguez-Trujíllo, Romén; Mills, Christopher A.; Samitier, J.; Gomila, Gabriel

doi:10.1007/s10404-006-0113-8

Cited by 74 publications

(48 citation statements)

References 24 publications

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“…Moreover, it was shown that the device was capable of analyzing as many as 17 000 cells/particles per second with an accuracy comparable to that of commercial flow cytometers. Rodriguez-Trujillo et al [43] presented a low-cost microcoulter counter fabricated from PMDS and glass and showed that the use of a hydrodynamic focusing technique enabled the device to probe particles with a wide range of diameters. The performance of the device was demonstrated experimentally via the counting of 20 mm diameter latex microbeads.…”

Section: Introductionmentioning

confidence: 99%

A high‐discernment microflow cytometer with microweir structure

Tsai

Lin

2008

Electrophoresis

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Using a simple and reliable isotropic wet etching process, we fabricated a microflow cytometer in which cells/particles are concentrated in the center of the sample stream using a 2-D hydrodynamic focusing technique and an microweir structure. Having focused the cells/particles, they are detected and counted using a LIF method. The experimental and numerical results confirm the effectiveness of the hydrodynamic sheath flows in squeezing the cells/particles into a narrow stream in the horizontal X-Y plane. Furthermore, it is shown numerically that the microweir structure results in the separation of the cells/particles in the vertical X-Z plane such that they pass through the detection region in a sequential fashion and can therefore be counted with a high degree of precision. The experimental results obtained using fluorescent polystyrene beads with diameters of 5 and 10 microm, respectively, confirm the suitability of the proposed device for microfluidic applications requiring the high-precision counting of particles or cells within a sample flow.

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Section: Introductionmentioning

confidence: 99%

A high‐discernment microflow cytometer with microweir structure

Tsai

Lin

2008

Electrophoresis

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“…First, electrical methods (referred to as Coulter counters) extract information from the modulation of the electrical resistance in an orifice due to the passage of the particle through the orifice. 13 A fine pore manufactured on a quartz substrate can measure colloids as small as 87 nm in diameter.…”

Section: Introductionmentioning

confidence: 99%

Measuring number‐concentrations of nanoparticles and viruses in liquids on‐line

Du¹,

Kendall²,

Morris³

et al. 2010

J of Chemical Tech & Biotech

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BACKGROUND: The surge of studies on artificial and natural nanoparticles has revealed a new world for engineering and life sciences, but in most instances, the small scale has made their number-concentration determination in liquids a challenging problem. Former success has mostly been limited to special particles measured by indirect techniques. A new approach is required for this determination to facilitate the production and application of nanoparticles in different systems.

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“…With two symmetric RPS channels on a microfluidic chip and a differential amplifier, this detection limit can be reduced to 1% [34,35]. To reduce clogging, the effective sampling aperture can be reduced without reducing the actual aperture by reducing the conductivity of the sheath flow [36,37]. With the differential amplifier, or the less conductive sheath fluid, a pore in the order of 5 lm enables the sizing of single vesicles with a diameter of 50 nm to 5 lm.…”

Section: Flow Cytometrymentioning

confidence: 99%

Innovation in detection of microparticles and exosomes

Pol

Coumans

Varga

et al. 2013

Journal of Thrombosis and Haemostasis

226

194

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Summary. Cell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body fluids such as blood. Although such vesicles have gained strong clinical and scientific interest, their detection is difficult because many vesicles are extremely small with a diameter of less than 100 nm, and, moreover, these vesicles have a low refractive index and are heterogeneous in both size and composition. In this review, we focus on the relatively high throughput detection of vesicles in suspension by flow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and we will discuss their applicability and limitations. Finally, we discuss four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small-angle X-ray scattering (SAXS), and anomalous SAXS. These methods are currently being explored to study vesicles and are likely to offer novel information for future developments.

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Low cost micro-Coulter counter with hydrodynamic focusing

Cited by 74 publications

References 24 publications

A high‐discernment microflow cytometer with microweir structure

A high‐discernment microflow cytometer with microweir structure

Measuring number‐concentrations of nanoparticles and viruses in liquids on‐line

Innovation in detection of microparticles and exosomes

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