Low-Cost Optical Mapping Systems for Panoramic Imaging of Complex Arrhythmias and Drug-Action in Translational Heart Models

Lee, Peter; Calvo, Conrado J.; Alfonso-Almazán, José Manuel; Quintanilla, Jorge G.; Chorro, Francisco J.; Yan, Ping; Loew, Leslie M.; Filgueiras-Rama, David; Millet, José

doi:10.1038/srep43217

Cited by 36 publications

(52 citation statements)

References 44 publications

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“…Raw signals are displayed (RV = blue, LV = red), which were used to depict the action potential -calcium transient coupling time (Figure 5C), activation and duration time (Figure 5D), electrical and calcium restitution ( Figure 5E). For thick myocardial preparations, spatial filtering with kernel size ~3 mm x 3 mm is appropriate for epicardial action potential or calcium transient analysis 19,47 . Accordingly, high spatial resolution images (in the described setup 1240 x 1024 total, or 620 x 512 per channel, 6.5 µm pixel size) are often spatially binned during or post-acquisition ( Figure 5C).…”

Section: Representative Resultsmentioning

confidence: 99%

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Swift

Jaimes

McCullough

et al. 2019

JoVE

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Small animal models are most commonly used in cardiovascular research due to the availability of genetically modified species and lower cost compared to larger animals. Yet, larger mammals are better suited for translational research questions related to normal cardiac physiology, pathophysiology, and preclinical testing of therapeutic agents. To overcome the technical barriers associated with employing a larger animal model in cardiac research, we describe an approach to measure physiological parameters in an isolated, Langendorff-perfused piglet heart. This approach combines two powerful experimental tools to evaluate the state of the heart: electrophysiology (EP) study and simultaneous optical mapping of transmembrane voltage and intracellular calcium using parameter sensitive dyes (RH237, Rhod2-AM). The described methodologies are well suited for translational studies investigating the cardiac conduction system, alterations in action potential morphology, calcium handling, excitation-contraction coupling and the incidence of cardiac alternans or arrhythmias. Video Link The video component of this article can be found at https://www.jove.com/video/60472/ 18,19. Indeed, simultaneous dual imaging of voltage and calcium-sensitive fluorescent probes allows for the assessment of electrical activity, calcium handling and excitation-contraction coupling at the tissue level

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Section: Representative Resultsmentioning

confidence: 99%

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Swift

Jaimes

McCullough

et al. 2019

JoVE

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“…This configuration allowed us to reconstruct action potential transients and the spread propagation with highresolution. Even though the presented configuration may be useful as it is, it can be further optimized, and even lower its financial costs, as previously described (7).…”

Section: Discussionmentioning

confidence: 98%

A Single-sensor High-resolution Panoramic Optical Mapping Configuration for Simultaneous Non-overlapped Complete Atrial and Ventricular Parametric Imaging

Calvo

Guill

Tormos

et al. 2017

Computing in Cardiology Conference (CinC)

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Nowadays optical mapping (OM) is the primary method for imaging electrophysiologically relevant parameters from the outer surface of Langendorffperfused hearts. This technique has become essential for comprehensively understanding mechanisms of cardiac propagation during physiological activation, arrhythmia, and therapeutic antiarrhythmic interventions in translational hearts. Panoramic whole heart optical mapping systems, using either multiple cameras, plane mirrors or a combination of both, have been developed to overcome intrinsic visualization limitations to traditional single-sensor designs. However current panoramic OM systems are financially challenging for physiology and engineering research labs since require multiple sensors to completely map wide areas of the surface, which further results on overlapping regions and/or missing areas of interest due to the intrinsic shape of the heart. In this study, we propose and validate a new spatially resolved low-cost single-sensor configuration to simultaneously record atrial and ventricular function in relation to its anatomy without overlapping regions.

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“…We believe this is in large part because this technology has always required significant engineering experience, which has acted as a barrier to entry for many in the cardiac physiology community. There has been a recent effort to present a low-cost panoramic imaging system 33 . While it addresses the issue of cost, the effort still falls short of providing publicly available and curated software for acquisition, processing, and analysis of both anatomical and optical data acquired from the complex 3D surface of the heart.…”

Section: Discussionmentioning

confidence: 99%

RHYTHM: An Open Source Imaging Toolkit for Cardiac Panoramic Optical Mapping

Gloschat

Aras

Gupta

et al. 2018

Sci Rep

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Fluorescence optical imaging techniques have revolutionized the field of cardiac electrophysiology and advanced our understanding of complex electrical activities such as arrhythmias. However, traditional monocular optical mapping systems, despite having high spatial resolution, are restricted to a two-dimensional (2D) field of view. Consequently, tracking complex three-dimensional (3D) electrical waves such as during ventricular fibrillation is challenging as the waves rapidly move in and out of the field of view. This problem has been solved by panoramic imaging which uses multiple cameras to measure the electrical activity from the entire epicardial surface. However, the diverse engineering skill set and substantial resource cost required to design and implement this solution have made it largely inaccessible to the biomedical research community at large. To address this barrier to entry, we present an open source toolkit for building panoramic optical mapping systems which includes the 3D printing of perfusion and imaging hardware, as well as software for data processing and analysis. In this paper, we describe the toolkit and demonstrate it on different mammalian hearts: mouse, rat, and rabbit.

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Low-Cost Optical Mapping Systems for Panoramic Imaging of Complex Arrhythmias and Drug-Action in Translational Heart Models

Cited by 36 publications

References 44 publications

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

A Single-sensor High-resolution Panoramic Optical Mapping Configuration for Simultaneous Non-overlapped Complete Atrial and Ventricular Parametric Imaging

RHYTHM: An Open Source Imaging Toolkit for Cardiac Panoramic Optical Mapping

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