Low density lipoprotein adsorption on a titanium surface and its effect on osteoblast behaviors

Xu, Lina; Shao, Shui-yi; Zhu, Wenqing; Chen, Chao; Zhang, Songmei; Qiu, Jing

doi:10.1039/c9ra03173a

Cited by 3 publications

(2 citation statements)

References 43 publications

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“…The XPS spectra (figure 4) showed that peaks of Ti 2p and O 1s gradually declined with an increase in the concentration of SEMA4D, indicating that SEMA4D was immobilized onto the titanium surfaces, and surface oxides were covered by the complex [69]. Since protein molecules contain many functional groups, the C 1s spectra consist of different peaks [70].…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of the immobilization of SEMA4D on titanium surfaces in an endothelial cell/macrophage indirect coculture model

Liu¹,

Zhang²,

Tang³

et al. 2021

Biomed. Mater.

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In this study, we established a procedure to prepare a Semaphorin4D (SEMA4D)-immobilized titanium surface and explored its effects on macrophage behaviors in an endothelial cell/macrophage indirect coculture model. The SEMA4D-bovine serum albumin complex was immobilized onto a preprocessed poly L-lysine titanium surface through NaOH hydrothermal treatment and self-assembly technology. All titanium specimens were examined for surface microstructure, surface element composition, and surface wettability by field emission scanning electron microscopy, x-ray photoelectron spectroscopy (XPS), and water contact angle measurement, respectively. Subsequently, we constructed an endothelial cell/macrophage indirect coculture model and evaluated the activation of NF-κB signaling pathway and the expression of proinflammatory cytokines (TNFα, IL-6, and IL-1β) in macrophages. In XPS analysis, the SEMA4D-immobilized titanium surface appeared as a loose porous structure covered with uniform film, which exhibited better hydrophilicity than the control smooth titanium surface. In the indirect coculture model, SEMA4D attenuated the activation of NF-κB signaling pathway of lipopolysaccharide-stimulated THP-1 macrophages, thereby downregulating the expression of proinflammatory cytokines in macrophages. In conclusion, SEMA4D could be immobilized on titanium surfaces through NaOH hydrothermal treatment and self-assembly technology. Meanwhile, SEMA4D immobilization altered the characteristics of the titanium surfaces, which negatively regulated macrophage behaviors in the endothelial cell/macrophage indirect coculture model.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of the immobilization of SEMA4D on titanium surfaces in an endothelial cell/macrophage indirect coculture model

Liu¹,

Zhang²,

Tang³

et al. 2021

Biomed. Mater.

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“…A clinical study found a negative correlation between LDL-C and lumbar bone density in individuals aged 20–59 years [ 4 ]. Furthermore, an in vitro study demonstrated that LDL adsorption negatively regulated osteoblast behaviors, such as adhesion, proliferation and differentiation [ 5 ]. These studies suggest the need for further research into the relationship between LDL-C and osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

The Causal Relationship between PCSK9 Inhibitors and Osteoporosis Based on Drug-Targeted Mendelian Combined Mediation Analysis

Zhang,

Ji,

Liu

et al. 2024

Calcif Tissue Int

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PCSK9 inhibitors have been shown to lower serum low density lipoprotein cholesterol (LDL-C) levels and are considered integral in the treatment of cardiovascular diseases. However, the potential association between PCSK9 inhibitors and osteoporosis is unclear now. In this study, drug-targeted mendelian randomization (MR) was utilized in conjunction with mediation analysis including bone mineral density (BMD), total 25-hydroxyvitamin D (T25(OH)D) levels and calcium supplementation to investigate the causal relationship between PCSK9 inhibitors and osteoporosis. The LDL-C level was chosen as the exposure variable in a sample size of 173,082 individuals. We conducted a MR analysis on the relationship between PCSK9 inhibitors and osteoporosis, elucidating the mediators involved. Utilizing the inverse variance weighted (IVW) method, we found the risk of osteoporosis was reduced by 0.6% in those who used PCSK9 inhibitors compared with non-users (OR: 0.994, 95%CI: 0.991–0.998, P < 0.001). In people aged 30–45 years, the risk of low BMD was 1.176 times higher among PCSK9 inhibitor users compared to non-users (OR: 1.176, 95%CI: 1.017–1.336, P = 0.045). Conversely, people aged 45–60 years who used PCSK9 inhibitors had a 14.9% lower risk of low BMD compared to non-users (OR: 0.851, 95%CI: 0.732–0.968, P = 0.007). Mediation analysis revealed that 43.33% of the impact of PCSK9 inhibitors on osteoporosis was mediated through BMD levels, with the remaining 56.67% being a direct effect. Effects of PCSK9 inhibitors on BMD levels varied in different ages. In addition, the risk of high serum T25(OH)D levels were 1.091 times among PCSK9 inhibitor users compared to non-users (OR: 1.091, 95%CI: 1.065–1.112, P < 0.001), providing valuable insights for clinicians.

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Protein adsorption on TiO2 nanostructures and its effects on surface topography and bactericidal performance

Zhao

Liu

et al. 2022

Applied Surface Science

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Low density lipoprotein adsorption on a titanium surface and its effect on osteoblast behaviors

Abstract: This study aims to investigate the adsorption of low density lipoprotein (LDL) on a titanium surface and to explore its effect on osteoblast behaviors.

Cited by 3 publications

References 43 publications

Anti-inflammatory effects of the immobilization of SEMA4D on titanium surfaces in an endothelial cell/macrophage indirect coculture model

Anti-inflammatory effects of the immobilization of SEMA4D on titanium surfaces in an endothelial cell/macrophage indirect coculture model

The Causal Relationship between PCSK9 Inhibitors and Osteoporosis Based on Drug-Targeted Mendelian Combined Mediation Analysis

Protein adsorption on TiO2 nanostructures and its effects on surface topography and bactericidal performance

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