Low-density lipoprotein receptor-related protein 2 gene is associated with egg-quality traits in dwarf layers

Zhang, Yuanyuan; Hou, Zhuocheng; Chen, Z. X.; Zheng, Jiangxia; Chen, S. R.; Qu, Lujiang; Li, J. Y.; Xu, Guiyun; Yang, Ning

doi:10.3382/ps.2011-01751

Cited by 12 publications

(12 citation statements)

References 18 publications

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“…) on chromosomes 1, 9, 13 and 23; and one in the central part of chromosome 7 from a candidate gene approach (Zhang et al . ). The published QTL regions on chromosomes 1 and 2 were close to some of the SNPs suggested by our study (Table ).…”

Section: Discussionmentioning

confidence: 97%

Genome‐wide association study for egg production and quality in layer chickens

Wolc

Arango

Jankowski³

et al. 2014

J Animal Breeding Genetics

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Discovery of genes with large effects on economically important traits has for many years been of interest to breeders. The development of SNP panels which cover the whole genome with high density and, more importantly, that can be genotyped on large numbers of individuals at relatively low cost, has opened new opportunities for genome-wide association studies (GWAS). The objective of this study was to find genomic regions associated with egg production and quality traits in layers using analysis methods developed for the purpose of whole genome prediction. Genotypes on over 4500 birds and phenotypes on over 13,000 hens from eight generations of a brown egg layer line were used. Birds were genotyped with a custom 42K Illumina SNP chip. Recorded traits included two egg production and 11 egg quality traits (puncture score, albumen height, yolk weight and shell colour) at early and late stages of production, as well as body weight and age at first egg. Egg weight was previously analysed by Wolc et al. (2012). The Bayesian whole genome prediction model--BayesB (Meuwissen et al. 2001) was used to locate 1 Mb regions that were most strongly associated with each trait. The posterior probability of a 1 Mb window contributing to genetic variation was used as the criterion for suggesting the presence of a quantitative trait locus (QTL) in that window. Depending upon the trait, from 1 to 7 significant (posterior probability >0.9) 1 Mb regions were found. The largest QTL, a region explaining 32% of genetic variance, was found on chr4 at 78 Mb for body weight but had pleiotropic effects on other traits. For the other traits, the largest effects were much smaller, explaining <7% of genetic variance, with regions on chromosomes 2, 12 and 17 explaining above 5% of genetic variance for albumen height, shell colour and egg production, respectively. In total, 45 of 1043 1 Mb windows were estimated to have a non-zero effect with posterior probability > 0.9 for one or more traits.

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Section: Discussionmentioning

confidence: 97%

Genome‐wide association study for egg production and quality in layer chickens

Wolc

Arango

Jankowski³

et al. 2014

J Animal Breeding Genetics

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“…Eggshell breaking strength and eggshell thickness were measured using the Egg Multi Tester EMT-5200 (Robotmation Co. Ltd). Measurement methods were as previously published [ 18 ]. After measurement, descriptive statistics were calculated for all individuals ( Fig 2 ).…”

Section: Methodsmentioning

confidence: 99%

Integrating Transcriptome and Genome Re-Sequencing Data to Identify Key Genes and Mutations Affecting Chicken Eggshell Qualities

et al. 2015

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Eggshell damages lead to economic losses in the egg production industry and are a threat to human health. We examined 49-wk-old Rhode Island White hens (Gallus gallus) that laid eggs having shells with significantly different strengths and thicknesses. We used HiSeq 2000 (Illumina) sequencing to characterize the chicken transcriptome and whole genome to identify the key genes and genetic mutations associated with eggshell calcification. We identified a total of 14,234 genes expressed in the chicken uterus, representing 89% of all annotated chicken genes. A total of 889 differentially expressed genes were identified by comparing low eggshell strength (LES) and normal eggshell strength (NES) genomes. The DEGs are enriched in calcification-related processes, including calcium ion transport and calcium signaling pathways as reveled by gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis. Some important matrix proteins, such as OC-116, LTF and SPP1, were also expressed differentially between two groups. A total of 3,671,919 single-nucleotide polymorphisms (SNPs) and 508,035 Indels were detected in protein coding genes by whole-genome re-sequencing, including 1775 non-synonymous variations and 19 frame-shift Indels in DEGs. SNPs and Indels found in this study could be further investigated for eggshell traits. This is the first report to integrate the transcriptome and genome re-sequencing to target the genetic variations which decreased the eggshell qualities. These findings further advance our understanding of eggshell calcification in the chicken uterus.

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“…At the age of 40 weeks, eggs were collected in 3 consecutive days from each hen for the measurement of egg quality. Egg weight (EW), eggshell weight (ESW), eggshell strength (ESS) and eggshell thickness (EST) were measured within 12 h after collecting eggs, as described in our previous publications [28,29], except for eggshell weight measurement. Eggshell weight refers to the wet eggshell which still contained the eggshell membranes with adhering water and albumen.…”

Section: Methodsmentioning

confidence: 99%

“…They were normalized using the expression values for the β-actin gene to obtain the relative expression of target genes. The relative gene expression was analyzed by the double standard curves method [28,29,32]. All the expression values were transformed using log2 when conducting T-Test.…”

Section: Methodsmentioning

confidence: 99%

The sodium channel gene family is specifically expressed in hen uterus and associated with eggshell quality traits

Fan

Hou

et al. 2013

BMC Genet

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BackgroundEggshell quality is important for the poultry industry. During eggshell formation a mass of inorganic minerals is deposited. The Sodium Channel (SCNN1) gene family plays an essential role in cation transportation. The objective of this study was to investigate the pattern of expression of members of the SCNN1 gene family, their variation and their effects on eggshell quality.ResultThe highest expression of SCNN1a, SCNN1b, and SCNN1g genes were in the active uterus during eggshell mineralization, while SCNN1d showed its highest expression level in the quiescent uterus (no egg present). Nineteen candidate SNPs from the four genes were genotyped in a population of 338 White Leghorn layers. Association analysis between SNPs (haplotypes/diplotypes) and eggshell traits was performed. Among seven significant SNPs, five SNPs were associated with eggshell strength, eggshell thickness, eggshell percentage or/and egg weight, while the other two SNPs within SCNN1d were only associated with eggshell percentage. These SNPs had a 0.25-6.99% contribution to phenotypic variance, depending on the trait. In haplotype analysis, SCNN1b and SCNN1d were associated with egg weight. The SCNN1b and SCNN1g were significantly associated with eggshell weight while only SCNN1g explained 2.04% of phenotypic variance. All the alleles of the members of SCNN1 gene family were associated with eggshell percentage and eggshell thickness, and others members had an association with eggshell strength except for SCNN1a. The contribution of different haplotypes of the SCNN1 gene family to eggshell phenotypic variance ranged from 0.09% to 5.74%.ConclusionsOur study indicated that the SCNN1 gene family showed tissue expression specificity and was significantly associated with eggshell traits in chicken. This study provides evidence that genetic variation in members of the sodium channel can influence eggshell quality.

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Low-density lipoprotein receptor-related protein 2 gene is associated with egg-quality traits in dwarf layers

Cited by 12 publications

References 18 publications

Genome‐wide association study for egg production and quality in layer chickens

Genome‐wide association study for egg production and quality in layer chickens

Integrating Transcriptome and Genome Re-Sequencing Data to Identify Key Genes and Mutations Affecting Chicken Eggshell Qualities

The sodium channel gene family is specifically expressed in hen uterus and associated with eggshell quality traits

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