Low-dose effects on thyroid disruption in zebrafish by long-term exposure to oxytetracycline

Yu, Kuo-Tsong; Li, Xiaoyong; Qiu, Yushu; Zeng, Xiaobo; Yu, Xiaogang; Wang, Weiye; Yi, Xiaoping; Huang, Lisu

doi:10.1016/j.aquatox.2020.105608

Cited by 46 publications

(19 citation statements)

References 44 publications

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“…Similarly, Zhou et al [ 41 ] have proved that exposure to oxytetracycline can induce adverse effects in zebrafish gut health. Meanwhile, Yu et al [ 95 ] revealed that long-term exposure to oxytetracycline can cause thyroid dysfunction and affect the growth and development of zebrafish. Both the triiodothyronine (T3) and thyroid-stimulating hormone (TSH) contents were altered; therefore, after treatment, the T3 contents were significantly enhanced and TSH secretion was reduced.…”

Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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The overuse of antibiotics combined with the limitation of wastewater facilities has resulted in drug residue accumulation in the natural environment. Thus, in recent years, the presence of antibiotic residues in the environment has raised concerns over the potential harmful effects on ecosystems and human health. The in vivo studies represent an essential step to study the potential impact induced by pharmaceutical exposure. Due to the limitations of traditional vertebrate model systems, zebrafish (Danio rerio) has recently emerged as a promising animal model to study the toxic effects of drugs and their therapeutic efficacy. The present review summarizes the recent advances made on the toxicity of seven representative classes of antibiotics, namely aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, tetracyclines and polyether antibiotics, in zebrafish, as well as the combined effects of antibiotic mixtures, to date. Despite a significant amount of the literature describing the impact of single antibiotic exposure, little information exists on the effects of antibiotic mixtures using zebrafish as an animal model. Most of the research papers on this topic have focused on antibiotic toxicity in zebrafish across different developmental stages rather than on their efficacy assessment.

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Section: Effects Of Individual Antibiotics In Zebrafishmentioning

confidence: 99%

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

et al. 2021

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“…It can be hypothesized that the LCs of OTC (or any other antibiotic released in the environment) could also result in similar dysbiosis of the intestinal microbiome and produce adverse effects. 16S rRNA gene sequencing-based microbial community analyses showed no significant change in the zebrafish gut microbial diversity after exposure to 100-420 ng/L OTC (Almeida et al, 2019;Li et al, 2020;Kayani et al, 2021). Using shotgun metagenomics, our previous study showed that the enrichment of multiple flavobacterial species occurred after exposure to OTC (1,000-5,000 ng/L) in the zebrafish (Kayani et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Longitudinal analysis of exposure to a low concentration of oxytetracycline on the zebrafish gut microbiome

Kayani

Qiu

et al. 2022

Front. Microbiol.

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Oxytetracycline, a widely produced and administered antibiotic, is uncontrollably released in low concentrations in various types of environments. However, the impact of exposure to such low concentrations of antibiotics on the host remains poorly understood. In this study, we exposed zebrafish to a low concentration (5,000 ng/L) of oxytetracycline for 1 month, collected samples longitudinally (Baseline, and Days 3, 6, 9, 12, 24, and 30), and elucidated the impact of exposure on microbial composition, antibiotic resistance genes, mobile genetic elements, and phospholipid metabolism pathway through comparison of the sequenced data with respective sequence databases. We identified Pseudomonas aeruginosa, a well-known pathogen, to be significantly positively associated with the duration of oxytetracycline exposure (Adjusted P = 5.829e−03). Several tetracycline resistance genes (e.g., tetE) not only showed significantly higher abundance in the exposed samples but were also positively associated with the duration of exposure (Adjusted P = 1.114e−02). Furthermore, in the exposed group, the relative abundance of genes involved in phospholipid metabolism had also decreased. Lastly, we characterized the impact of exposure on zebrafish intestinal structure and found that the goblet cell counts were decreased (~82%) after exposure. Overall, our results show that a low concentration of oxytetracycline can increase the abundance of pathogenic bacteria and lower the abundance of key metabolic pathways in the zebrafish gut microbiome that can render them prone to bacterial infections and health-associated complications.

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“…In the above-mentioned study, for example, the presence of both antibiotics was associated with higher mortality of the fish when challenged with a pathogen (Zhou et al 2018). The same oxytetracycline, also in low dose, had a very strong effect on the development of the animals in other experiments (Yu et al 2020), indicating a high potential of the antibiotic to affect the fish's physiology via changes in its microbiota. Exposure of Nile tilapia Oreochromis niloticus to low doses of sulfamethoxazole and oxytetracycline only slightly changed microbial community and diversity, but chronic exposure to antibiotics impaired intestinal morphology, permeability and induced microbiota dysbiosis with a very strong negative impact on the fish's physiology, nutritional meta bolism and immune system (Limbu et al 2018).…”

Section: Antibioticsmentioning

confidence: 83%

Human impact on symbioses between aquatic organisms and microbes

Stock

Callens²,

Houwenhuyse

et al. 2021

Aquat. Microb. Ecol.

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Aquatic organisms rely on microbial symbionts for coping with various challenges they encounter during stress and for defending themselves against predators, pathogens and parasites. Microbial symbionts are also often indispensable for the host's development or life cycle completion. Many aquatic ecosystems are currently under pressure due to diverse human activities that have a profound impact on ecosystem functioning. These human activities are also ex pected to alter interactions between aquatic hosts and their associated microbes. This can directly impact the host's health and -given the importance and widespread occurrence of microbial symbiosis in aquatic systems -the ecosystem at large. In this review, we provide an overview of the importance of microbial symbionts for aquatic organisms, and we consider how the beneficial services provided by microbial symbionts can be affected by human activities. The scarcity of available studies that assess the functional consequences of human impacts on aquatic microbial symbioses shows that our knowledge on this topic is currently limited, making it difficult to draw general conclusions and predict future changes in microbial symbiont−host relationships in a changing world. To address this important knowledge gap, we provide an overview of ap proaches that can be used to assess the impact of human disturbances on the functioning of aquatic microbial symbioses.

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Low-dose effects on thyroid disruption in zebrafish by long-term exposure to oxytetracycline

Cited by 46 publications

References 44 publications

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Zebrafish as a Screening Model to Study the Single and Joint Effects of Antibiotics

Longitudinal analysis of exposure to a low concentration of oxytetracycline on the zebrafish gut microbiome

Human impact on symbioses between aquatic organisms and microbes

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