Low Dose Tcdd Affects Membrane Bound Ion Dependent Atpases in Mice Liver

Abstract

Present communication reports in vivo dose and duration dependent effects of a dioxin (2,3,7,8 TCDD) on few membrane bound ion dependent ATPases of mice hepatocytes. Two hypotheses were tested in this study. Separate groups of mice were exposed to two very low sublethal doses (0.004 mg/kg bw, 0.04 mg/kg bw) of TCDD by daily oral administration for 2, 4 and 6 days. Separate control groups were also maintained which received only corn oil, the vehicle. The results indicated a significant exposure duration dependent alterations in the membrane ATPases. No clear dose dependent effects were observed. The results suggested that the observed alteration in the enzyme activity was possibly due to the oxidative stress generated by TCDD on the membrane-bound ion-dependent ATPases. The results also indicated that the membrane bound hydrophilic ATPases were indeed influenced by the hydrophobic TCDD. However, the effects were possibly indirect through complex chain of reactions exhibited by the cells under in vivo conditions. ROS was suspected to have played an important role in the alterations of membrane transport systems and permeability which caused cellular injury, and thereby exhibiting exposure duration dependent effects.

Low Concentrations of PCB (Aroclor 1254) Alter Membrane Bound Ion Dependent ATPases in the Hepatocyte Cells of Mice

“…Therefore, one of the possibilities of indirect effect of Aroclor 1254 is oxidative stress which may alter the activity of membrane bound ion dependent ATPases by producing free radicals. It may also be possible that PCBs accumulated in the intracellular space and alter the activity of membrane bound ion-dependent ATPases which altered the membrane permeability [21][22][23][24].…”

In Vivo Dose and Duration Dependent Effects of a Dioxin (2,3,7,8 TCDD) on Few Lysosomal Enzymes in Mice Brain

“…2,3,7,8 TCDD is an environmental pollutant, produced during the improper burning of paper, pulp, wastes [1][2][3] and tend to accumulate in the adipose tissues of animals including human, exposed through different environmental sources [4,5] . Dioxin like PCBs contaminated fish consumed by pregnant women reported developmental disorder and cognitive deficits in infants [6][7][8] .…”

Low Doses of a PCB (Aroclor 1254) Affect the Body Weight by Decreasing the Activity of Glucose-6-Phosphatase in the Liver and Kidney Cells of Mice

“…Many observations across such studies include impaired gluconeogenesis, decrease in body weight, and reductions in the glucose-6-phosphatase activity and gene expression of PEPCK [35,32,40,10,39]. In the given broader distribution, and environmental contamination by, PCBs, compared to TCDD [41,42], the effects of PCB on hepatic and renal glucose metabolism are potentially important.…”