Low‐energy Mg²⁺‐induced temperature transitions in liver microsomes

Abstract

The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg'+. Addition of Mg2+ yields three partially reversible phase transitions at 18,27 and 32"C, respectively. A character of Mg'+-induced rearrangements in a membrane and their relation to a catalytic function of a cytochrome P-450-dependent enzymatic system is discussed. Rat liver microsome Differential scanning microcalorimetry Fluorescent probe