Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma

Ma, Guoliang; Zhang, Fengmei; Dong, Xueguang; Wang, Xiaoli; Ren, Yi

doi:10.3892/etm.2016.3014

Cited by 11 publications

(12 citation statements)

References 27 publications

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“…In oesophageal squamous cell carcinoma, miR-202 is poorly expressed in tumour tissues and inversely correlated with the degree of cell differentiation and lymph node metastasis. In addition, miR-202 is decreased in the peripheral blood of oesophageal squamous cell carcinoma patients and significantly associated with the development, invasion and metastasis of oesophageal squamous cell carcinoma (37). Moreover, low expression levels of miR-202 are observed in multiple myeloma (24), colorectal cancer (22), hepatocellular carcinoma (25), lung (38) and cervical cancer (39).…”

Section: Discussionmentioning

confidence: 99%

“…Another study revealed that the restoration of miR-202 expression suppresses cervical cancer cell growth and metastasis (39). Ma et al reported that ectopic expression of miR-202 inhibits cell proliferation, migration and invasion and induces cell apoptosis of oesophageal squamous cell carcinoma (37,41). Meanwhile, Jiang et al (38) demonstrated that the upregulation of miR-202 decreases cell proliferation and improves the G0/G1 cell cycle arrest and apoptosis in lung cancer.…”

Section: Discussionmentioning

confidence: 99%

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MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

et al. 2017

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Glioma is the most common and aggressive type of primary malignant brain tumour. Increasing evidence has revealed that microRNAs play important roles in multiple biological processes related to glioma occurrence, development, diagnosis, treatment and prognosis. MicroRNA-202 (miR-202) has been studied in several types of human cancer, whereas the biological roles of miR-202 in glioma remain unknown. The present study, aimed to investigate the expression, clinical significance and biological roles of miR-202 in glioma, as well as its underlying molecular mechanism. We found that miR-202 was significantly downregulated in glioma tissues and cell lines. Low miR-202 expression was associated with Karnofsky performance status (KPS) score and World Health Organization (WHO) grade of glioma patients. Functional assays revealed that ectopic expression of miR-202 inhibited cell proliferation, migration and invasion of glioma. In addition, metadherin (MTDH) was identified as a direct target gene of miR-202 in glioma through bioinformatic analysis, luciferase reporter assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. Furthermore, MTDH expression was upregulated and negatively correlated with miR-202 expression in clinical glioma tissues. MTDH knockdown had similar roles to miR-202 overexpression in glioma cells. Rescue experiments revealed that upregulation of MTDH reversed the suppression of glioma cell growth and metastasis by miR-202. Moreover, miR-202 impaired the PI3K/Akt and Wnt/β-catenin pathways. These results highlight the tumour-suppressive effect of miR-202 in glioma, thereby suggesting that miR-202 may be a potential therapeutic target for the treatment of patients with this malignancy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

et al. 2017

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“…MiR-202 may also play an important role in different cancers. For example, miR-202 suppressed cell proliferation in EC by targeting FOXR2 [10], and low miR-202 expression was shown to contribute to migration and invasion of esophageal squamous cell carcinoma cells [11]. However, whether miR-202 regulates EMT and cell migration and invasion in EC remains unknown.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-202 inhibits cell migration and invasion through targeting FGF2 and inactivating Wnt/β-catenin signaling in endometrial carcinoma

Chen

Xing

et al. 2019

Bioscience Reports

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Recently, many mircroRNAs (miRNAs) involved in the development and progression of cancer have been reported to regulate cell growth and metastasis, including microRNA-202 (miR-202). The purpose of the present study was to elucidate the effect of miR-202 on endometrial carcinoma (EC) cell migration and invasion. First, qRT-PCR showed that miR-202 was down-regulated in EC tissues, which was associated with poor prognosis in EC patients. Functionally, transwell assay indicated that miR-202 inhibited cell migration and invasion in EC cells. In addition, miR-202 also blocked epithelial–mesenchymal transition (EMT) through suppressing N-cadherin and Vimentin expressions and promoting E-cadherin expression. Moreover, the dual-luciferase reporter assay showed that fibroblast growth factor 2 (FGF2) is a direct target gene for miR-202 in EC cells. Furthermore, up-regulation of FGF2 attenuated the inhibitory effect of miR-202 on cell migration and invasion in EC. Besides that, miR-202 inactivated the Wnt/β-catenin signaling by suppressing β-catenin expression in EC. In conclusion, miR-202 inhibited cell migration and invasion by targeting FGF2 and inactivating the Wnt/β-catenin signaling in EC.

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“…There are reports, which has been shown that the expression of miR-202 is downregulated in cervical cancer [28], lung cancer [21], hepatocellular carcinoma [29], colorectal cancer [20], and multiple myeloma [30]. In oesophageal squamous cell carcinoma, the miR-202 showed low level of expression in tumour tissues and its expression was signi cantly correlated (negative correlation) with lymph node metastasis and cell differentiation [31]. It is documented that the miR-202 expression in gastric cancer is also downregulated in tumour tissues and showed an inverse correlation with age and tumour size [22].…”

Section: Discussionmentioning

confidence: 99%

“…In lung cancer, restoration of miR-202 increased apoptosis and G0/G1 cell cycle arrest and suppressed cell proliferation in lung cancer cell line [21]. Studies on oesophageal squamous cell carcinoma showed that upregulation of miR-202 could suppresses invasion, migration, proliferation and also, increase cell apoptosis [31,33]. Together, these ndings clarify that the miR-202 could be introduced as an important biomarker in these cancers and may serve as a potential therapeutic target.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of miRNA-202 promotes apoptosis and inhibits migration of glioma cell line via downregulation of ROCK1 gene

Behzadi

Hatami

Alian

et al. 2020

Preprint

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Background: We evaluated role(s) of miR-202 in glioma cell lines, its effect on ROCK1 expression, and also evaluation of apoptosis and migration of human glioma cell line after transfection with miR-202 mimics and inhibitors. Material and methods: The cell lines were transfected with mimic, inhibitor and NC of miR-202. Reverse transcription polymerase chain reaction (RT-PCR) was conducted to evaluate the expression of miR‐202 and ROCK1 . Western blot was performed to detect the protein level of ROCK1. Furthermore, MTT and wound healing assay were performed to evaluate the effects of miR-202 on apoptosis and migration of human glioma cell line, respectively. Results: miR-202 showed a significantly decrease in human glioma cell lines, compared with the NHA cell line (P<0.05). The ROCK1 expression was significantly upregulated in glioma cell lines, compared with the NHA cell line ( P <0.05). Furthermore, a negative correlation was observed between expression of ROCK1 and miR-202 ( P =0.01, r=-0.426). The mRNA and protein levels of ROCK1 were decreased in U87 cell line in miR-202 mimics group, compared with mimic NC group (P<0.05). In addition, apoptosis was significantly increased in miR-202 mimics, compared with the NC group in U87 cell line at 72 and 96 h (P<0.05). Furthermore, invasion showed a significant decrease in miR-202 mimic group, compared with U87 cell line at 24 and 48 h (P<0.05). Conclusions: The miR-202 could serve as a tumour-suppressor miRNA in glioma. Therefore, targeting ROCK1 by miR-202 may increase improve disease outcome and could be considered as a potential therapeutic target for glioma patients.

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Low expression of microRNA-202 is associated with the metastasis of esophageal squamous cell carcinoma

Cited by 11 publications

References 27 publications

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

MicroRNA-202 inhibits cell proliferation, migration and invasion of glioma by directly targeting metadherin

MicroRNA-202 inhibits cell migration and invasion through targeting FGF2 and inactivating Wnt/β-catenin signaling in endometrial carcinoma

Upregulation of miRNA-202 promotes apoptosis and inhibits migration of glioma cell line via downregulation of ROCK1 gene

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