Low-flow Perfusion of Guinea Pig Isolated Hearts With 26°C Air-saturated Lifor Solution for 20 Hours Preserves Function and Metabolism

Abstract

Background-Donor human hearts cannot be preserved for >5 hours between explantation and recipient implantation. A better approach is needed to preserve transplantable hearts for longer periods, ideally at ambient conditions for transport. We tested whether Lifor solution could satisfactorily preserve guinea pig isolated hearts perfused at low flow with no added oxygen at room temperature for 20 hours.

Continuous Real-time Viability Assessment of Kidneys Based on Oxygen Consumption

“…Consequently, determining WOOCR could be useful for quantifying organ viability even when measurements are conducted during cold preservation (4–8°C). Calculation of oxygen consumption rate (OCR) has been applied before in the study of the heart,5 the liver,6 and the kidney 7-9. It has also been shown that OCR is a reliable and useful measure of pancreatic islet quality and can be used to predict islet transplant outcome 10-12…”

Protective Effect of Lifor Solution in Experimental Renal Ischemia-Reperfusion Injury

“…[11, 12] Stowe et al first evaluated Lifor in a model of low-flow perfused guinea pig hearts. Under ambient air and temperature conditions, perfusion with Lifor for 10 hours provided superior protection against cardiac injury compared to UW perfusion.…”

“…[11, 12] Recently, Lifor was shown to preserve guinea pig hearts in a coronary recirculation system for up to 20 hours and to improve renal perfusion in a porcine model of room temperature perfusion. [12, 13] However, the effect of Lifor on renal function and renal tubular epithelial survival following either warm IR injury or cold storage is unknown. Therefore, the current study aimed to compare the effectiveness of Lifor to UW solution in protecting rat kidneys from warm IR and cold storage injury and to determine the effect of Lifor on renal tubular cell apoptosis.…”

Machine perfusion in organ transplantation

“…Left ventricular function seems better preserved after hypothermic [59][60][61] and normothermic [62,63] machine perfusion compared with SCS. Clinical trials were started in 2007 in USA (PROCEED) [64] and Europe (PROTECT) [65], with only preliminary data reported so far.…”