Low follicular fluid IL-6 levels in IVF patients are associated with increased likelihood of clinical pregnancy

Altun, Tugba; Jindal, Sangita; Greenseid, Keri; Shu, Jun; Pal, Lubna

doi:10.1007/s10815-010-9502-8

Cited by 48 publications

(36 citation statements)

References 44 publications

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“…TNFa in FF has been proposed to be related with oocyte quality and IVF outcomes , which is consistent with our results. Previous work suggested that elevated levels of IL6 in FF may be detrimental to implantation (Altun et al 2011). However, we did not observe this relationship in our study.…”

Section: Discussioncontrasting

confidence: 98%

Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis

Choi¹,

Cho²,

Seo³

et al. 2015

Reproduction

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The aim of this study was to compare intrafollicular biomarkers of thiol-redox system and chronic inflammation in infertile patients with and without endometriosis, and examine correlations between biomarkers and IVF outcomes. The study included 65 patients receiving IVF: 31 patients with endometriosis vs 34 patients without endometriosis. Follicular fluid (FF) was obtained from a single-dominant follicle during oocyte retrieval and stored at K70 8C. Malondialdehyde, superoxide dismutase, glutathione (GSH), glutathione peroxidase 3 (GPX3), thioredoxin (TRX), TRX-binding protein 2 (TBP2), and peroxiredoxin-4 levels were measured in the FF samples by ELISAs as biomarkers of oxidative stress. The inflammatory cytokines interleukin 1 beta (IL1b), IL6, IL8, and tumor-necrosis factor alpha (TNFa) were also measured by ELISAs. GSH levels were significantly lower in the endometriosis group compared with the controls. TBP2 levels were significantly higher in the endometriosis group. IL6, IL8, and TNFa levels were significantly higher in the endometriosis group. The levels of all of the inflammatory cytokines positively correlated with the levels of TRX. GSH levels positively correlated with the number of high-quality embryos. GPX3 and TRX levels negatively correlated with the percentage of mature oocytes. TNFa levels negatively correlated with the cumulative embryo score per embryo. Logistic regression analysis revealed that the number of high-quality embryos was an independent factor predicting clinical pregnancy. In conclusion, there may be an imbalance in the thiol-redox system and increased levels of inflammatory cytokines in the intrafollicular microenvironment of infertile patients with endometriosis, which may affect the qualities of the oocyte and embryo. Reproduction (2015) 149 155-162

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Section: Discussioncontrasting

confidence: 98%

Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis

Choi¹,

Cho²,

Seo³

et al. 2015

Reproduction

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“…Reduced follicular fluid concentrations of some cytokines have been reported to be associated with successful IVF treatment cycles (Ostanin et al, 2007;Altun et al, 2011). It is possible that increased levels of some cytokines might impair the endometrial receptivity as no other differences were observed in either oocyte or follicular parameters.…”

Section: Serum (N=30) ->mentioning

confidence: 99%

“…It is possible that increased levels of some cytokines might impair the endometrial receptivity as no other differences were observed in either oocyte or follicular parameters. IL-6 levels tend to increase with age (Altun et al, 2011). On the other hand, positive associations with pregnancy rates have been described for the concentrations of a number of other interleukins as well as for TNF-␣ and G-CSF (Ostanin et al, 2007).…”

Section: Serum (N=30) ->mentioning

confidence: 99%

“…Changes in pro-and anti-inflammatory cytokine and growth factor concentrations in the ovary, as a consequence of gonadotropin stimulation, may therefore also negatively influence follicular function and thereby the oocyte quality. A recent study on follicular fluid (FF) reported reduced concentrations of IL-6 in the group of women who subsequently became pregnant in the corresponding IVF cycle compared with those who did not (Altun et al, 2011). Another investigation focusing on FF cytokine concentrations in relation to oocyte quality and pregnancy rates found positive associations for most of them, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Serum but not follicular fluid cytokine levels are increased in stimulated versus natural cycle IVF: a multiplexed assay study

Bersinger

Kollmann

Wolff

2014

Journal of Reproductive Immunology

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“…As cytokines are involved in the regulation of follicles and as the follicular fluid contains cytokine-producing immune cells [23], increased intrafollicular inflammatory cytokine concentrations might have an effect on follicular physiology, resulting in lower oocyte quality and thereby in reduced outcome of IVF therapies. This suggestion is clinically supported by Altun et al [24]. They have reported an increased likelihood for clinical pregnancies in patients with low intrafollicular IL-6 cytokine concentrations in women without endometriosis.…”

Section: Discussionmentioning

confidence: 53%

Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis

Bersinger²,

Mueller³

et al. 2017

J Assist Reprod Genet

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Purpose The aim of this study was to assess whether the intrafollicular cytokine profile in naturally developed follicles is different in women with endometriosis, possibly explaining the lower reproductive outcome in endometriosis patients. Methods A matched case-control study was conducted at a university-based infertility and endometriosis centre. The study population included 17 patients with laparoscopically and histologically confirmed endometriosis (rAFS stages II-IV), each undergoing one natural cycle IVF (NC-IVF) treatment cycle between 2013 and 2015, and 17 age-matched NC-IVF women without diagnosed endometriosis (control group). Follicular fluid and serum was collected at the time of follicle aspiration. The concentrations of inflammatory cytokines (IL-1β, IL-6, IL-8, IL-15, IL-18, TNF-α) and hormones (testosterone, estradiol, AMH) were determined in follicular fluid and serum by single or multiplexed immunoassay and compared between both groups. Results In the follicular fluid, IL-1β and IL-6 showed significantly (P < 0.001 and 0.01, respectively) higher median concentrations in the endometriosis group than in the control group and a tendency towards endometriosis severity (rAFS stage) dependence. The levels of the interleukins detectable in follicular fluid were significantly higher than those in the serum (P < 0.01). Follicular estradiol concentration was lower in severe endometriosis patients than in the control group (P = 0.036). Follicular fluid IL-1β and IL-6 levels were not correlated with estradiol in the same compartment in neither patient group. Conclusions In women with moderate and severe endometrioses, some intrafollicular inflammatory cytokines are upregulated and not correlated with intrafollicular hormone concentrations. This might be due to the inflammatory microenvironment in endometriosis women, affecting follicular function and thereby possibly contributing to the reproductive dysfunction in endometriosis.

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Low follicular fluid IL-6 levels in IVF patients are associated with increased likelihood of clinical pregnancy

Abstract: Lower follicular fluid IL-6 levels in IVF patients are associated with increased likelihood of clinical pregnancy. We hypothesize that endometrial receptivity is a likely target for any deleterious influences of elevated IL-6 levels.

Cited by 48 publications

References 44 publications

Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis

Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis

Serum but not follicular fluid cytokine levels are increased in stimulated versus natural cycle IVF: a multiplexed assay study

Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis

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