Low‐Frequency Electrical Stimulation Promotes Satellite Cell Activities to Facilitate Muscle Regeneration at an Early Phase in a Rat Model of Muscle Strain

Wang, Daan; Qing-Zheng, Li,; Jia, Dongming

doi:10.1155/2021/4218086

Cited by 5 publications

(2 citation statements)

References 22 publications

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“…This is the rst study to examine the effects of ES during prolonged MV using genetic analysis of the diaphragm muscle. Although there have been numerous studies related to ES-induced effects on muscle atrophy 21,23,24 , no study has analyzed the genetic changes caused by ES during prolonged MV. The main ndings of the present study are that 12-hour MV is reasonable for establishing a VIDD rat model and that ES favorably affects genes related to in ammatory cytokines, skeletal muscle, and internal respiration (oxygen transport and mitochondrial respiration) during prolonged MV.…”

Section: Discussionmentioning

confidence: 99%

Electrical stimulation of the diaphragm may counteract muscle degradation during prolonged mechanical ventilation: A pilot study using transcriptome analyses

Nakai

Hirata

Furue

et al. 2023

Preprint

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Ventilator-induced diaphragm dysfunction (VIDD), a dysfunction of the diaphragm muscle caused by prolonged mechanical ventilation (MV), is an important factor that hinders successful weaning from ventilation. We aimed to evaluate the effects of electrical stimulation of the diaphragm muscle on genetic changes during 12 hours of MV (E-V12). Rats were divided into four groups: control, 12-hour MV, sham operation, and E-V12 groups. Transcriptome analysis using an RNA microarray revealed that 12-hour MV caused upregulation of genes promoting muscle atrophy and downregulation of genes facilitating muscle synthesis, suggesting that 12-hour MV is a reasonable method for establishing a VIDD rat model. Of the genes upregulated by 12-hour MV, 18 genes were not affected by the sham operation but were downregulated by E-V12. These included genes related to catabolic processes, inflammatory cytokines, and skeletal muscle homeostasis. Of the genes downregulated by 12-hour MV, 6 genes were not affected by the sham operation but were upregulated by E-V12. Those included genes related to oxygen transport and mitochondrial respiration. These results suggested that 12-hour MV shifted gene expression in the diaphragm muscle toward muscle degradation and that electrical stimulation counteracted this shift by suppressing catabolic processes and improving mitochondrial respiration.

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Section: Discussionmentioning

confidence: 99%

Electrical stimulation of the diaphragm may counteract muscle degradation during prolonged mechanical ventilation: A pilot study using transcriptome analyses

Nakai

Hirata

Furue

et al. 2023

Preprint

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“…It should be noted that skeletal muscle-resident stem cells (also known as muscle satellite cells) contribute to muscle regeneration in response to various injuries [40][41][42][43][44][45][46][47]. However, a previous study demonstrated that lifelong reduction of satellite cells neither accelerated nor exacerbated sarcopenia and that satellite cells did not contribute to the maintenance of muscle size or fiber type composition during aging, but that their loss may contribute to age-related muscle fibrosis [48].…”

Section: Discussionmentioning

confidence: 99%

Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice

Goto

Inoue

Piao

et al. 2021

Stem Cells International

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Background. We recently demonstrated that proliferin-1 (PLF-1) functions as an apoptotic cell-derived growth factor and plays an important role in vascular pathobiology. We therefore investigated its role in muscle regeneration in response to cardiotoxin injury. Methods and Results. To determine the effects of PLF-1 on muscle regeneration, we used a CTX-induced skeletal muscle injury model in 9-week-old male mice that were administered with the recombinant PLF-1 (rPLF-1) or neutralizing PLF-1 antibody. The injured muscles exhibited increased levels of PLF-1 gene expression in a time-dependent manner. On day 14 after injury, rPLF-1 supplementation ameliorated CTX-induced alterations in muscle fiber size, interstitial fibrosis, muscle regeneration capacity, and muscle performance. On day 3 postinjury, rPLF-1 increased the levels of proteins or genes for p-Akt, p-mTOR, p-GSK3α/β, p-Erk1/2, p-p38MAPK, interleukin-10, Pax7, MyoD, and Cyclin B1, and it increased the numbers of CD34+/integrin-α7+ muscle stem cells and proliferating cells in the muscles and/or bone marrow of CTX mice. An enzyme-linked immunosorbent assay revealed that rPLF-1 suppressed the levels of plasma tumor necrosis factor-α and interleukin-1β in CTX mice. PLF-1 blocking accelerated CTX-related muscle damage and dysfunction. In C2C12 myoblasts, rPLF-1 increased the levels of proteins for p-Akt, p-mTOR, p-GSK3α/β, p-Erk1/2, and p-p38MAPK as well as cellular functions; and these effects were diminished by the depletion of PLF-1 or silencing of its mannose-6-phosphate receptor. Conclusions. These findings demonstrated that PLF-1 can improve skeletal muscle repair in response to injury, possibly via the modulation of inflammation and proliferation and regeneration, suggesting a novel therapeutic strategy for the management of skeletal muscle diseases.

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Surface‐Embedding of Mo Microparticles for Robust and Conductive Biodegradable Fiber Electrodes: Toward 1D Flexible Transient Electronics

et al. 2023

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Fiber-based implantable electronics are one of promising candidates for in vivo biomedical applications thanks to their unique structural advantages. However, development of fiber-based implantable electronic devices with biodegradable capability remains a challenge due to the lack of biodegradable fiber electrodes with high electrical and mechanical properties. Here, a biocompatible and biodegradable fiber electrode which simultaneously exhibits high electrical conductivity and mechanical robustness is presented. The fiber electrode is fabricated through a facile approach that incorporates a large amount of Mo microparticles into outermost volume of a biodegradable polycaprolactone (PCL) fiber scaffold in a concentrated manner. The biodegradable fiber electrode simultaneously exhibits a remarkable electrical performance (≈43.5 𝛀 cm −1 ), mechanical robustness, bending stability, and durability for more than 4000 bending cycles based on the Mo/PCL conductive layer and intact PCL core in the fiber electrode. The electrical behavior of the biodegradable fiber electrode under the bending deformation is analyzed by an analytical prediction and a numerical simulation. In addition, the biocompatible properties and degradation behavior of the fiber electrode are systematically investigated. The potential of biodegradable fiber electrode is demonstrated in various applications such as an interconnect, a suturable temperature sensor, and an in vivo electrical stimulator.

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Low‐Frequency Electrical Stimulation Promotes Satellite Cell Activities to Facilitate Muscle Regeneration at an Early Phase in a Rat Model of Muscle Strain

Cited by 5 publications

References 22 publications

Electrical stimulation of the diaphragm may counteract muscle degradation during prolonged mechanical ventilation: A pilot study using transcriptome analyses

Electrical stimulation of the diaphragm may counteract muscle degradation during prolonged mechanical ventilation: A pilot study using transcriptome analyses

Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice

Surface‐Embedding of Mo Microparticles for Robust and Conductive Biodegradable Fiber Electrodes: Toward 1D Flexible Transient Electronics

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