Low-frequency, green sonoextraction of antioxidants from tree barks of Hungarian woodlands for potential food applications

Agarwal, Charu; Hofmann, Thilo; Visi-Rajczi, Eszter; Pásztory, Zoltán

doi:10.1016/j.cep.2020.108221

Cited by 15 publications

(9 citation statements)

References 42 publications

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“…The methanol extract from pistachios shows a high TPC of 0.56 GAE g/g with a relatively high antioxidant activity, but it should be noted that it is obtained with a volatile organic solvent extraction agent that is not environmentally friendly [21]. As shown by Charu et al [22], from Hungarian black alder bark using different solvents the authors have obtained extracts with much lower results in terms of the total polyphenol content, 21.25 and 15.14 GAE mg/g in 80% ethanol water and in 80% acetone water extracts, respectively. Comparing the results obtained in our work, which are on average 560 GAE mg/g and obtained with water as a solvent, the results are much higher.…”

Section: Resultsmentioning

confidence: 98%

Antioxidant Activity of Different Extracts from Black Alder (Alnus glutinosa) Bark with Greener Extraction Alternative

Lauberts

Pāls

2021

Plants

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Phenolic compounds isolated from plant biomass consist of bioactive components showing a wide range of benefits for humans, including antioxidant, antimicrobial or anti-inflammatory effects. This paper presents the potential value of black alder (Alnus glutinosa (L.) Gaertn. (Betulaceae)) bark for the production of biologically active substances, despite its current use as a low value fuel source. Most of the extraction methods employ neat organic solvents to obtain extracts with a high antioxidant potential from biomass. The aim of this work is to show the advantages and disadvantages of the extraction process by taking into account the principles of ‘green chemistry’ and replacing the organic solvents with ‘green’ solvent water. Using the advantages of accelerated solvent extraction (ASE), it has been shown that the use of deionized water has the prospect of replacing organic solvents. In the case of the one-step water extraction, the total polyphenol content (TPC) varies from 0.55 to 0.62 Gallic acid equivalent (GAE) g/g in the extracts, depending on the temperature, whereas with the result of the sequential extraction with the organic solvents, the TPC content of the 40% (v:v) ethanol extracts ranges from 0.39 to 0.61 GAE g/g, depending on the temperature. The influence of the total polyphenol content and the total proanthocyanidin content on the antioxidant activity is shown. The antioxidant activity (IC50, mg/L) of the extracts obtained with the organic solvents in the (2,2-diphenyl-1-picrylhydrazyl) DPPH• test varies from 4.05 to 9.58, depending on the temperature in the range of 70–150 °C, respectively, while the results obtained with the deionized water showed promising results in the range of 6.33–7.36 in the temperature range of 70–150 °C, respectively. The extraction with the deionized water showed that approximately 90% of the substances in the extracts obtained with the organic solvents by sequential extraction are possible to obtain as deionized water extracts.

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Section: Resultsmentioning

confidence: 98%

Antioxidant Activity of Different Extracts from Black Alder (Alnus glutinosa) Bark with Greener Extraction Alternative

Lauberts

Pāls

2021

Plants

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“…Compared to oil products, the bark under study, as well as the biomass generally, has too little hydrogen, too much oxygen, a lower fraction of carbon and a significant amount of ash [6] (Table 2). [6] 83-87 10-14 0.1-1.5 0.1-2 1 The relative standard deviation for three repeated tests was ≤3%; 2 calculated by difference.…”

Section: Composition Of Aspen (Populus Tremula) and Pine (Pinus Sylve...mentioning

confidence: 97%

“…Plant biomass research nowadays is focused on the conversion of its processing residues into products with a minimum amount of waste by using the biorefinery concept. The bark comprises about 5-20% of the wood, and its annual production as a byproduct of the chemical or mechanical processing of forest biomass is estimated to be around 300-400 million m 3 globally [1,2]. In the vast majority of cases, it is simply left in the forest or burnt, recycled as a fuel source or insulation material or used in horticulture for weed suppression and moisture retention.…”

Section: Introductionmentioning

confidence: 99%

“…It has a small output but is characterized by a high added value [4][5][6]. Due to the accumulation of biologically active compounds in the bark of trees, which results from plant evolution as a response to biotic and abiotic stresses, the bark-derived extracts have interesting pharmacological and cosmetic activities [1,7]. The composition and properties of bark extracts depend on tree species as well as on the extraction technique and the extraction solvents that are used [8].…”

Section: Introductionmentioning

confidence: 99%

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Microwave-Assisted Water Extraction of Aspen (Populus tremula) and Pine (Pinus sylvestris L.) Barks as a Tool for Their Valorization

Pāls

Lauberte

Ponomarenko

et al. 2022

Plants

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The barks of aspen (Populus tremula) and pine (Pinus sylvestris) are byproducts of wood processing, characterized by their low economic value. In the present study, microwave-assisted one-cycle water extraction was explored as a tool for the valorization of this biomass as a source of biologically active compounds. The microwave extractor of the original construction equipped with a pressurized extraction chamber and a condenser section was used. The microwave-assisted extraction (MAE), specially including dynamic dielectric heating up to 70 °C followed by 30 min of isothermal heating, promoted the isolation of salicin from aspen bark, allowing for the obtention of a two-times-higher free salicin concentration in water extracts (−14% vs. 7%) reached by multi-cycle accelerated solvent extraction (ASE), which is an advanced technique used as a reference. The MAE of pine bark with dynamic heating up to 90–130 °C, avoiding the isothermal heating step, allowed for the obtention of a 1.7-times-higher concentration of proantocyanidin dimers-tetramers, a 1.3-times-higher concentration of catechin and a 1.2-times-higher concentration of quinic acid in water extracts in comparison to a more time- and solvent-consuming ASE performed at the same temperature. The biological activity of the obtained extracts was characterized in terms of their ability to inhibit xahntine oxidase enzyme, which is a validated target for the therapeutic treatment of hyperuricemia.

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“…On the other hand, in this study, mostly the glycoside conjugates of polyphenolic compounds were found rather than their respective free aglycones. The ratio of glycoside conjugates and their respective free aglycones in bark extracts depends on many factors including age, type of sample collection (ratio of inner and outer bark), storage and drying, as well as on extraction solvent and method [53]. The significance of polyphenolic compounds in contributing to the antibacterial properties of wild cherry bark extracts was reported by Oyetayo and Bada (2017) [47].…”

Section: Polyphenolic Composition Of Bark Extractsmentioning

confidence: 99%

In vitro antioxidant and antibacterial activities with polyphenolic profiling of wild cherry, the European larch and sweet chestnut tree bark

Agarwal

Hofmann

Vršanská

et al. 2021

Eur Food Res Technol

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This study is a comparative investigation of antioxidant and antibacterial properties of tree bark extracts of three common European species, Prunus avium L., Larix decidua Mill. and Castanea sativa Mill. The bioactive compounds present in the bark were recovered in 80% aqueous ethanol using ultrasound as the green extraction method. The antioxidant potential of the extracts was assessed with multiple biochemical assays: total phenol content (TPC) expressed in gallic acid equivalent (GAE), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) expressed in trolox equivalent (TE), and ferric reducing ability of plasma (FRAP) expressed in ascorbic acid equivalent (AAE). Sweet chestnut bark extract showed the highest antioxidant activity with TPC of 174.25 ± 16.95 mg GAE/g dry weight, DPPH (IC50) of 2.69 ± 0.03 μg/mL, ABTS of 739.65 ± 24.41 mg TE/g dry weight and FRAP of 207.49 ± 3.62 mg AAE/g dry weight. The antibacterial activity of the extracts was evaluated by disk diffusion test, minimal inhibitory concentration (MIC) assay and bacterial growth curves. Sweet chestnut bark extract gave IC50 values of 0.25 mg/mL and 1.00 mg/mL against E. coli and S. aureus, respectively. The polyphenolic profiling of the bark extracts was performed to identify the major compounds responsible for the bioactivities using high-performance liquid chromatography/tandem mass spectrometry (HPLC–MS/MS). The bark extracts were rich in natural antioxidants, thus holding tremendous potential for use as natural additives in food industry. Graphic abstract

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Low-frequency, green sonoextraction of antioxidants from tree barks of Hungarian woodlands for potential food applications

Cited by 15 publications

References 42 publications

Antioxidant Activity of Different Extracts from Black Alder (Alnus glutinosa) Bark with Greener Extraction Alternative

Antioxidant Activity of Different Extracts from Black Alder (Alnus glutinosa) Bark with Greener Extraction Alternative

Microwave-Assisted Water Extraction of Aspen (Populus tremula) and Pine (Pinus sylvestris L.) Barks as a Tool for Their Valorization

In vitro antioxidant and antibacterial activities with polyphenolic profiling of wild cherry, the European larch and sweet chestnut tree bark

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