Low frequency of HPV positivity in breast tumors among patients from south-central Poland

Abstract

Background Some studies suggest that Human Papilloma Virus (HPV) infection is important factor in carcinogenesis of breast tumors. This study’ objective was to analyze HPV prevalence in breast cancers of patients from south-central Poland. Materials and methods The study was performed based on archival paraffin embebbed and formalin fixed blocks in the group of 383 patients with breast cancer. HPV prevalence and its genotype were assessed, respectively by: nested PCR (with two groups of primers: PGMY09/PGMY11 and GP5+/GP6+), quantitative PCR (qPCR). Tumors were classified as HPV positive in case of at least one positive result in nested PCR and positive results in genotyping procedure. For all HPV positive tissues P16 immunostaining was applied in order to confirm active viral infection. Results In the group of 383 breast cancers, HPV positivity was found in 17 samples (4.4%) in nested PCR. All these samples were subjected to HPV genotyping. This analysis revealed presence of HPV type 16 into two tumors (0.5%). In these two cancers, P16 overexpression was reported. Conclusion In breast tumors of patients from south-central Poland in Poland, HPV positivity is demonstrated in very low percentage of cases.

Breast cancer association with oncogenic papillomaviruses: arguments pro and con

“…В дальнейшем B. Biesaga и соавт. сообщили о значительной гиперэкспрессии p16 INK4a в 2 ВПЧ16-положительных образцах протокового РМЖ III стадии и дали убедительные иммуногистохимические иллюстрации [22]. В целом представляется целесообразным продолжение исследований экспрессии p16 INK4a в ВПЧ-положительном РМЖ.…”