Low‑frequency ultrasound and microbubbles combined with simvastatin promote the apoptosis of MCF‑7 cells by affecting the LATS1/YAP/RHAMM pathway

Li, Haige; Chen, Chen; De-hang, Wang

doi:10.3892/mmr.2018.9273

Cited by 5 publications

(7 citation statements)

References 50 publications

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“…Microscopy-based techniques are ideal for managing multiple time scales since imaging rates can vary from real-time imaging (a few frames per second) allowing the evaluation of drug influx and cellular responses to high-speed (150 000 fps) imaging which can resolve the release of model drugs from microbubbles and streaming effects and even to ultra-high-speed imaging (up to 25 million fps), making it possible to resolve the microbubble oscillations during ultrasound exposure. , Other methods can be used to evaluate the consequences of microbubble–cell interactions during or after ultrasound treatment . For example, the integrity of cell membranes and intercellular junctions can be measured using electrical sensing techniques (TMC and TEER). , The proteomic and transcriptomic changes in the exposed tissue can be analyzed using Western blots, − ELISA assays, and PCR analysis. , The effects can be studied in further detail quantitatively using flow cytometry and qualitatively using electron and atomic force microscopy. Nevertheless, owing to this inherent complexity, the interpretation and integration of these different techniques remains a significant challenge.…”

Section: Part 2: Microbubble–cell Interactions To Release and Deliver...mentioning

confidence: 99%

The Role of Ultrasound-Driven Microbubble Dynamics in Drug Delivery: From Microbubble Fundamentals to Clinical Translation

et al. 2019

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In the last couple of decades, ultrasound-driven microbubbles have proven excellent candidates for local drug delivery applications. Besides being useful drug carriers, microbubbles have demonstrated the ability to enhance cell and tissue permeability and as a consequence, drug uptake herein. Notwithstanding the large amount of evidence for their therapeutic efficacy, open issues remain. Due to the vast amount of ultrasound-and microbubble-related parameters that can be altered, and the variability in different models, the translation from basic research to (pre-)clinical studies has been hindered. This review aims at connecting the knowledge gained from fundamental microbubble studies to the therapeutic efficacy seen in in vitro and in vivo studies, with an emphasis on a better understanding of the response of a microbubble upon exposure to ultrasound and its interaction with cells and tissues. More specifically, we address the acoustic settings and microbubble-related parameters i.e. bubble size and physico-chemistry of the bubble shell that play a key role in microbubble-cell interactions and in the associated therapeutic outcome. Additionally, new techniques that may provide additional control over the treatment, such as monodisperse microbubble formulations, tunable ultrasound scanners and cavitation detection techniques, are discussed. An in-depth understanding of the aspects presented in this work could eventually lead the way to more efficient and tailored microbubble-assisted ultrasound therapy in the future.

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Section: Part 2: Microbubble–cell Interactions To Release and Deliver...mentioning

confidence: 99%

The Role of Ultrasound-Driven Microbubble Dynamics in Drug Delivery: From Microbubble Fundamentals to Clinical Translation

et al. 2019

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“…LATS1 is an upstream protein of YAP and adjusts YAP by removing it from the nuclear chamber. 28 A study has showed that LATS proteins are notably down-expressed in breast cancer and non-small cell lung cancer. 29 Dephosphorylated YAP allows its entry into nucleus where it activates the transcription of proproliferative and anti-apoptotic targets.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in the RA‐V(1) group, after the blocking of Hippo signalling pathway, the expression of LATS1 and the extent of YAP phosphorylation decreased, the expression of Beclin 1 increased, expression of LC3‐II/I and Casepase‐3 increased, and the number of autophagic vesicles increased, thus inhibiting proliferative capacity and promoting apoptotic and autophagic ability. LATS1 is an upstream protein of YAP and adjusts YAP by removing it from the nuclear chamber . A study has showed that LATS proteins are notably down‐expressed in breast cancer and non‐small cell lung cancer .…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

Peng

Tan

et al. 2019

J Cellular Molecular Medi

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The role of long non‐coding RNAs (lncRNAs) in thyroid carcinoma (TC), the most frequent endocrine malignancy, has been extensively examined. This study investigated effect of interaction among lncRNA TNRC6C‐AS1, serine/threonine‐protein kinase 4 (STK4) and Hippo signalling pathway on TC. Initially, lncRNA TNRC6C‐AS1 expression in TC tissues was detected. To explore roles of lncRNA TNRC6C‐AS1, STK4 and Hippo signalling pathway in TC progression, their expressions were altered. Interaction between lncRNA TNRC6C‐AS1 and STK4, STK4 promoter methylation, or Hippo signalling pathway was verified. After that, a series of experiments were employed to evaluate in vitro ability of apoptosis, proliferation and autophagy of TC cells and in vivo tumorigenicity, and tumour growth of TC cells. lncRNA TNRC6C‐AS1 was highly expressed while STK4 was poorly expressed in TC tissues. LncRNA TNRC6C‐AS1 promoted the STK4 methylation and down‐regulated STK4 expression, which further activated the Hippo signalling pathway. STK4 silencing was observed to promote the proliferation ability of TC cells, inhibit the apoptosis and autophagy abilities, as well as enhance the tumorigenicity and tumour growth. Moreover, the in vitro proliferation ability as well as the in vivo tumorigenicity and tumour growth of TC cells were inhibited after the blockade of Hippo signalling pathway, while the apoptosis and autophagy abilities were promoted. The results demonstrate that the lncRNA TNRC6C‐AS1 increases STK4 promoter methylation to down‐regulate STK4 expression, thereby promoting the development of TC through activation of Hippo signalling pathway. It highlights that lncRNA TNRC6C‐AS1 may be a novel therapeutic target for the treatment of TC.

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“…First, in vitro studies have shown that application of low-intensity ultrasound can regulate the Hippo pathway and YAP/TAZ. [190][191][192] Second, in vitro studies in Schwann cells have shown that ultrasound regulates signaling pathways upstream of the Hippo pathway. 193,194 Finally, extensive studies provide evidence that low-intensity ultrasound activates and promotes Schwann cell proliferation, a major outcome of the Hippo pathway (reviewed in 195 ).…”

Section: Mechanical Based Therapiesmentioning

confidence: 99%

The Hippo pathway: Horizons for innovative treatments of peripheral nerve diseases

Feltri

Weaver

Belin

et al. 2021

J Peripheral Nervous Sys

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Initially identified in Drosophila, the Hippo signaling pathway regulates how cells respond to their environment by controlling proliferation, migration and differentiation. Many recent studies have focused on characterizing Hippo pathway function and regulation in mammalian cells. Here, we present a brief overview of the major components of the Hippo pathway, as well as their regulation and function. We comprehensively review the studies that have contributed to our understanding of the Hippo pathway in the function of the peripheral nervous system and in peripheral nerve diseases. Finally, we discuss innovative approaches that aim to modulate Hippo pathway components in diseases of the peripheral nervous system.

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Low‑frequency ultrasound and microbubbles combined with simvastatin promote the apoptosis of MCF‑7 cells by affecting the LATS1/YAP/RHAMM pathway

Cited by 5 publications

References 50 publications

The Role of Ultrasound-Driven Microbubble Dynamics in Drug Delivery: From Microbubble Fundamentals to Clinical Translation

The Role of Ultrasound-Driven Microbubble Dynamics in Drug Delivery: From Microbubble Fundamentals to Clinical Translation

Long non‐coding RNA TNRC6C‐AS1 promotes methylation of STK4 to inhibit thyroid carcinoma cell apoptosis and autophagy via Hippo signalling pathway

The Hippo pathway: Horizons for innovative treatments of peripheral nerve diseases

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