Low-frequency vibration promotes AMPK-mediated glucose uptake in 3T3-L1 adipocytes

Haba, Daijiro; Nakagami, Gojiro; Minematsu, Takeo; Sanada, Hiromi

doi:10.1016/j.heliyon.2021.e07897

Cited by 6 publications

(16 citation statements)

References 21 publications

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“…LDH is used as an indicator of necrotic cell death (Cox et al, 2021; Haba et al, 2021). Cells were cultured for indicated times, and the culture media were collected for cytotoxicity analysis.…”

Section: Methodsmentioning

confidence: 99%

Obeticholic acid improved triptolide/lipopolysaccharide‐induced hepatotoxicity by inhibiting caspase‐11‐GSDMD pyroptosis pathway

Liang

Zhou

Yuan

et al. 2022

J of Applied Toxicology

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This study was designed to investigate the potential role of farnesoid X receptor (FXR) in abnormal bile acid metabolism and pyroptosis during the pathogenesis of triptolide (TP)/lipopolysaccharide (LPS)-induced hepatotoxicity. Moreover, the protective effect of obeticholic acid (OCA) was explored under this condition. In vivo, female C57BL/6 mice were administrated with OCA (40 mg/kg bw, intragastrical injection) before (500 μg/kg bw, intragastrical injection)/LPS (0.1 mg/kg bw, intraperitoneal injection) administration. In vitro, AML12 cells were treated with TP (50 nM) and TNF-α (50 ng/ml) to induce hepatotoxicity; GW4064 (5 μM) and cholestyramine (CHO) (0.1 mg/ml and 0.05 mg/ml) were introduced to explain the role of FXR/total bile acid (TBA) in it. Serum TBA level was significantly elevated, which was induced by FXR suppression. And both GW4064 and CHO intervention presented remarkable protective effects against TP/TNF-α-induced NLRP3 upregulation and pyroptosis pathway activation. Pre-administration of FXR agonist OCA successfully attenuated TP/LPS-induced severe liver injury by reducing serum bile acids accumulation and inhibiting the activation of caspase-11-GSDMD (gasdermin D) pyroptosis pathway.We have drawn conclusions that TP aggravated liver hypersensitivity to LPS and inhibited FXR-SHP (small heterodimer partner) axis, which was served as endogenous signals to activate caspase-11-GSDMD-mediated pyroptosis contributing to liver injury. OCA alleviated TP/LPS-induced liver injury accompanied by inhibiting caspase-11-GSDMD-mediated pyroptosis pathway and decreased serum TBA level.The results indicated that FXR might be an attractive therapeutic target for TP/LPSinduced hepatotoxicity, providing an effective strategy for drug-induced liver injury.

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Section: Methodsmentioning

confidence: 99%

Obeticholic acid improved triptolide/lipopolysaccharide‐induced hepatotoxicity by inhibiting caspase‐11‐GSDMD pyroptosis pathway

Liang

Zhou

Yuan

et al. 2022

J of Applied Toxicology

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“…Hyperglycemia originated from IR in patients with diabetes are predisposed to developing chronic wounds such as DFUs. Because IR could delay wound healing though interplay among impaired tissue regeneration, vasculopathy, neuropathy and in ammation [10,11].…”

Section: Introductionmentioning

confidence: 99%

Association between Triglyceride glucose index and severity of diabetic foot ulcers in type2 diabetes mellitus

Zhang

Chen

Wang

et al. 2023

Preprint

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Background: Triglyceride glucose (TyG) index is a good surrogate biomarker to evaluate insulin resistance (IR). The study aimed to investigate whether the TyG index is related to the severity of diabetic foot ulcers (DFUs) in patients with type2 diabetes mellitus (T2DM). Methods: A total of 1059 T2DM patients were enrolled in this observational, retrospective, single-center study. TyG index was calculated as ln[fasting triglycerides (mg/dl)×fasting glucose (mg/ dl)/2]. The severity of DFUs was classified into mild-to-moderate DFUs (Wagner grade score <3) and severe DFUs (Wagner grade score ≥3) based on Wagner classification. Patients were stratified according to the tertiles of TyG index. Logistic regression models were implemented to explore the association between TyG index and the severity of DFUs. Subgroup analyses were used to verify the reliability of results. Results: Compared with the reference lowest TyG tertile (T1), the highest tertile (T3) was associated with 0.377-fold increased risk of prevalence of severe DFUs (odds ratio [OR] 1.377, 95% confidence interval [CI] 1.017-1.865) (P =0.039). After adjusting for potential confounders, the multivariable-adjusted OR and 95% CI were 1.506 (1.079-2.103) (P =0.016) in patients with highest tertile. Moreover, subgroup analyses indicated that the association was stronger among men, patients with age ≥ 65 years, duration of diabetes more than 10 years, or without PAD. Conclusions: Elevated TyG index is independently associated with severity of DFUs even after adjusting conventional confounders.

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“…15,16 Drawing from these insights, we explored the effects of LLFV on improving glycometabolism and inflammation. Consequently, we previously demonstrated that LLFV of 50 Hz at 1000-1500 mVpp, which refers to the voltage applied to the vibrator, for 40 min/day for 5 days promoted AMP-activated protein kinase (AMPK)-mediated glucose transporter 4 (GLUT4) translocation and glucose uptake in 3T3-L1 adipocytes, 17 and LLFV of 600-1000 mVpp for 40 min/day promoted wound healing in the early phase of the healing process in a hyperglycemic rat model. 18 LLFV improved local glycometabolism, including promotion of GLUT4 expression and inhibition of methylglyoxal production-a major precursor of advanced glycation end-products.…”

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confidence: 99%

“…1,22 Additionally, the LLFV intensity for enhancing glucose uptake in adipocytes and promoting blood flow appeared to be different. 11,17 Therefore, we conceived to develop an intelligent vibration dressing to promote DFU wound healing via improvement of glucose uptake and blood flow at the wound site and autonomous optimization of vibration intensity based on the patient's insulin resistance and vasodilatory capacity using artificial intelligence (Figure 1).…”

mentioning

confidence: 99%

“…This wearable vibration dressing can supply LLFV of 50 Hz, with a vibration intensity similar to that used in our earlier research. 17,18 We investigated its effects on hyperglycemic wound healing in awakened rats, focusing on inflammation and blood flow. This study aimed to investigate the effects of the wearable vibration dressing on local blood circulation, inflammation, and full-thickness wound healing in hyperglycemic rats.…”

mentioning

confidence: 99%

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Efficacy of wearable vibration dressings on full‐thickness wound healing in a hyperglycemic rat model

Haba,

Ohmiya,

Sekino

et al. 2023

Wound Repair Regeneration

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Local low‐frequency vibration promotes blood flow and wound healing in hard‐to‐heal diabetic foot ulcers (DFUs). However, vibration treatment is challenging in patients with DFUs due to wound management difficulties and low adherence. Consequently, developing wearable self‐care devices becomes imperative for effective wound healing. This study introduces a wearable vibration dressing and assesses its impact on wound healing in hyperglycemic rats. Low‐frequency vibration at 52 Hz was applied to the wound for 40 min/day in awake rats. Relative wound areas on post‐wounding days (PWDs) 4–7 were significantly smaller and the wound closure rate was significantly higher in the vibration group than in the control group (p < 0.05, respectively). The total haemoglobin at baseline and after vibration on post‐wounding day 7 was significantly larger in the vibration group than in the control group (p < 0.05). On PWD 7, the thickness of the granulation tissue was significantly higher in the vibration group than in the control group (p < 0.05). Moreover, the number of blood vessels at the wound site and vascular endothelial growth factor A protein expression were significantly higher in the vibration group than in the control group (p < 0.05, respectively). The ratio of (CD68+/iNOS+)/(CD163+) macrophages in the vibration group was significantly lower than that in the control group (p < 0.05). These results indicate the potential of wearable vibration dressings as new self‐care devices that can promote angiogenesis and blood flow, improve inflammation, and enhance wound healing in DFUs.

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Low-frequency vibration promotes AMPK-mediated glucose uptake in 3T3-L1 adipocytes

Cited by 6 publications

References 21 publications

Obeticholic acid improved triptolide/lipopolysaccharide‐induced hepatotoxicity by inhibiting caspase‐11‐GSDMD pyroptosis pathway

Obeticholic acid improved triptolide/lipopolysaccharide‐induced hepatotoxicity by inhibiting caspase‐11‐GSDMD pyroptosis pathway

Association between Triglyceride glucose index and severity of diabetic foot ulcers in type2 diabetes mellitus

Efficacy of wearable vibration dressings on full‐thickness wound healing in a hyperglycemic rat model

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