Low genetic variability in <i><scp>S</scp>clerotinia sclerotiorum</i> populations from common bean fields in <scp>M</scp>inas <scp>G</scp>erais <scp>S</scp>tate, <scp>B</scp>razil, at regional, local and micro‐scales

Lehner, Miller da Silva; Júnior, Trazilbo J. Paula; Júnior, B. T. Hora; Teixeira, Hudson; Vieira, Rogério Faria; Carneiro, José Eustáquio Souza; Mizubuti, Eduardo S. G.

doi:10.1111/ppa.12322

Cited by 43 publications

(84 citation statements)

References 41 publications

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“…Thus, caution must be applied, because the populations of S. sclerotiorum are variable, although at low levels under Brazilian conditions (Lehner et al, 2015a). In general, our results suggest that Cornell 605 is the most appropriate source of physiological resistance to white mold for the southern region, whereas Cornell 605 and A195 are somewhat superior to G122 for the mid-western and southern regions.…”

Section: Resultsmentioning

confidence: 62%

“…PCR reactions were performed with Type-it Microsatellite PCR kit as described by the manufacturer (Qiagen). We used primers labeled with fluorescent dye (6-FAM, NED, HEX; Applied Biosystems) and an automatic sequencer ABI PRISM 3100 Genetic Analyser (Applied Biosystem) for measuring the size of the alleles (Lehner et al, 2015a). Each S. sclerotiorum isolate was assigned to a haplotype using GenoDive software (Meirmans and Tienderen, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…The isolates from Minas Gerais were purified by hyphal-tipping and those from the states of Goiás, São Paulo, Paraná, and Santa Catarina by a single ascospore as described by Lehner et al (2015a). Isolates were genotyped using the microsatellite loci 7-2, 8-3, 9-2, 12-2, 13-2, 36-4, 42-4, 92-4, 106-4 and 114-4 (Sirjusingh and Kohn, 2001).…”

Section: Methodsmentioning

confidence: 99%

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Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum

Lehner

Júnior

Vieira

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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White mold caused by the fungus Sclerotinia sclerotiorum is the most yield-limiting disease of common bean in Brazil. To date, there has been no commercial cultivar resistant to this disease. In a greenhouse we evaluated white mold resistance sources (Cornell 605, A195 and G122) against eight isolates of S. sclerotiorum from five Brazilian states. A Brazilian cultivar (BRSMG Madrepérola) and a susceptible check (Beryl) were used as control. Treatments were arranged in factorial combinations (5 × 8) in a completely random design with four replicates.Disease severity was assessed on a rating scale of 1-to-9 together with lesion length, which was used to determine an area under the disease progress curve (AUDPC). Polymorphisms detected in ten microsatellite loci were used to assess variability between the isolates. Each isolate was a distinct haplotype; they formed a genetic tree with two clusters. One cluster was formed by three isolates collected from the states of Minas Gerais and São Paulo (southeastern); the others, by isolates from Paraná, Santa Catarina (southern), Goiás (Mid-western), and again, Minas Gerais.Genotype × isolate interaction was significant. In general, Beryl was more susceptible than BRSMG Madrepérola. Considering the AUDPC and/or the white mold reaction score, Cornell 605 exhibited more physiological resistance than BRSMG Madrepérola to seven isolates, A195 to five isolates, and G122 to two isolates. Our results suggest that Cornell 605 is the best source of resistance to white mold for the southern region, whereas Cornell 605 and A195 are somewhat superior to G122 for the southeastern and mid-western regions.

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Section: Resultsmentioning

confidence: 62%

Section: Methodsmentioning

confidence: 99%

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Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum

Lehner

Júnior

Vieira

et al. 2016

Sci. agric. (Piracicaba, Braz.)

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“…Our ϕ = 0.287, which is directly comparable to the ϕ from both Lehner et al (2015); by the fact that genotypes are shared among populations in Brazil (Fig. 2).…”

supporting

confidence: 62%

Genetic variation and structure of Sclerotinia sclerotiorum populations from soybean in Brazil

Pannullo

Kamvar

Miorini

et al. 2018

Preprint

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“…Although, some papers report a small variability of this pathogen in Brazil (LEHNER et al, 2015;LEHNER et al, 2016 a).…”

Section: Introductionmentioning

confidence: 99%

Reaction of common bean lines derived from recurrent selection for white mold resistance and aggressiveness of Sclerotinia sclerotirum isolates

Leite¹,

Figueiredo²,

Dias³

et al. 2017

Biosci. J.

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ABSTRACT:The objectives of this study were to verify the resistance of common bean lines derived from recurrent selection for white mold resistance and to identify those more stable to different isolates; to compare the aggressiveness of different Sclerotinia sclerotiorum isolates; and to verify isolates x lines interaction. Fifteen common bean lines were evaluated, twelve derived from recurrent selection for white mold resistance, one non-adapted source of resistance (Cornell 605), one moderately resistant and adapted (VC-16) and one susceptible to white mold (Corujinha). Ten isolates were used to inoculate the common bean lines through the straw test. A total of ten experiments were performed, one for each isolate. The randomized complete block design with three replications was used in each experiment. Each plot had five plants inoculated in two main branches, therefore the plot data was the average of the ten evaluations through a scale of nine grades. Diallel analysis were used to estimate the general reaction capacity (lines) and general aggressiveness capacity (isolates) to measure the resistance to white mold and the aggressiveness of the isolates, respectively. The GGE biplot analysis was used to group the common bean lines based on their resistance alleles and identify those more instable to the isolates. The resistance of the lines P4 and P10 was similar to Cornell 605, and they had stable reaction to different isolates and "Carioca" grain type. The lines of the advanced cycles of recurrent selection accumulated more favorable alleles than those of the first cycles, confirming the efficiency of the recurrent selection to increase white mold resistance in common bean. In addition, it was identified more aggressive isolates, UFLA 109 and UFLA 116, and a small magnitude of isolates x lines interaction, indicating a predominance of the horizontal resistance of the lines.

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Low genetic variability in Sclerotinia sclerotiorum populations from common bean fields in Minas Gerais State, Brazil, at regional, local and micro‐scales

Cited by 43 publications

References 41 publications

Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum

Reaction of sources of resistance to white mold to microsatellite haplotypes of Sclerotinia sclerotiorum

Genetic variation and structure of Sclerotinia sclerotiorum populations from soybean in Brazil

Reaction of common bean lines derived from recurrent selection for white mold resistance and aggressiveness of Sclerotinia sclerotirum isolates

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