Low impedance and highly transparent microelectrode arrays (MEA) for in vitro neuron electrical activity probing

Susloparova, Anna; Halliez, Sophie; Bégard, Séverine; Colin, Morvane; Buée, Luc; Pecqueur, Sébastien; Alibart, Fabien; Thomy, V.; Arscott, S.; Pallecchi, Emiliano; Coffinier, Yannick

doi:10.1016/j.snb.2020.128895

Cited by 30 publications

(29 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…while keeping a perfect balance between both receptors (Figure A). Then, we investigated the effect of control and the activated-astrocyte supernatant on TNFR1 and TNFR2 on neuronal networks at DIV12 , (Figure A). The supernatant of control astrocytes did not statistically disturb the balance between TNFR1 and TNFR2 nor the fluorescence signal, suggesting that healthy astrocytes did not affect both receptors.…”

Section: Results and Discussionmentioning

confidence: 99%

“…To reproduce mechanical injuries, stretchable chambers were made with a thin PDMS membrane stuck to a PDMS block. The PDMS membrane of 150 μm thick was obtained by spin coating, , whereas the PDMS block was molded over a silanized Teflon mold. The field deformation of the elastic PDMS membranes was estimated by printing fluorescent protein (FITC-BSA) circles of 2000 μm 2 on the membrane of the device.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks

Lantoine

Procès

Villers

et al. 2021

ACS Chem. Neurosci.

Self Cite

View full text Add to dashboard Cite

Deformation, compression, or stretching of brain tissues cause diffuse axonal injury (DAI) and induce structural and functional alterations of astrocytes, the most abundant cell type in the brain. To gain further insight into the role of mechanically activated astrocytes on neuronal networks, this study was designed to investigate whether cytokines released by mechanically activated astrocytes can affect the growth and synaptic connections of cortical neuronal networks. Astrocytes were cultivated on elastic membranes and subjected to repetitive mechanical insults, whereas well-defined protein micropatterns were used to form standardized neuronal networks. GFAP staining showed that astrocytes were mechanically activated after two cycles of stretch and mesoscale discovery assays indicated that injured astrocytes released four major cytokines. To understand the role of these cytokines, neuronal networks were cultured with the supernatant of healthy or mechanically activated astrocytes, and the individual contribution of the proinflammatory cytokine tumor necrosis factor-α (TNF-α) was studied. We found that the supernatant of two-cycle stretched astrocytes decreased presynaptic terminals and indicated that TNF-α must be considered a key player of the synaptic loss. Furthermore, our results indicate that cytokines released by injured astrocytes significantly modulate the balance between TNFR1 and TNFR2 receptors by enhancing R2 receptors. We demonstrated that TNF-α is not involved in this process, suggesting a predominant role of other secreted cytokines. Together, these results contribute to a better understanding of the consequences of repetitive astrocyte deformations and highlight the role of inflammatory signaling pathways in synaptic plasticity and modulation of TNFR1 and TNFR2 receptors.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks

Lantoine

Procès

Villers

et al. 2021

ACS Chem. Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the relatively high impedance to obtain high transparency leads to high background noise and a low signal-to-noise ratio. Poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT: PSS) ( Koutsouras et al, 2017 ; Kshirsagar et al, 2019 ; Susloparova et al, 2021 ) is a new interfacial material for modern bioelectronics and is better than ITO in some aspects, such as conductivity and flexibility. It is still facing some challenges like longevity and multichannel ( Liang et al, 2021 ).…”

Section: Advanced Materials For Ex Vivo Non Studiesmentioning

confidence: 99%

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Zhang

Rong

Bian

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Increasing population is suffering from neurological disorders nowadays, with no effective therapy available to treat them. Explicit knowledge of network of neurons (NoN) in the human brain is key to understanding the pathology of neurological diseases. Research in NoN developed slower than expected due to the complexity of the human brain and the ethical considerations for in vivo studies. However, advances in nanomaterials and micro-/nano-microfabrication have opened up the chances for a deeper understanding of NoN ex vivo, one step closer to in vivo studies. This review therefore summarizes the latest advances in lab-on-chip microsystems for ex vivo NoN studies by focusing on the advanced materials, techniques, and models for ex vivo NoN studies. The essential methods for constructing lab-on-chip models are microfluidics and microelectrode arrays. Through combination with functional biomaterials and biocompatible materials, the microfluidics and microelectrode arrays enable the development of various models for ex vivo NoN studies. This review also includes the state-of-the-art brain slide and organoid-on-chip models. The end of this review discusses the previous issues and future perspectives for NoN studies.

show abstract

“…In the past ten years, conductive polymers including polypyrrole (PPy) [ 22 ] and poly(3,4-ethylenedioxythiophene) (PEDOT) have attracted much attention as interface materials. In particular, PEDOT has been widely used in neural interfaces due to its biocompatibility and chemical stability [ 23 , 24 , 25 , 26 ]. The electrochemical deposition of PEDOT forms a positively charged framework, which provides accommodation for negatively charged dopants to reach charge balance.…”

Section: Introductionmentioning

confidence: 99%

A Neural Sensor with a Nanocomposite Interface for the Study of Spike Characteristics of Hippocampal Neurons under Learning Training

Deng

Luo

et al. 2022

Biosensors

View full text Add to dashboard Cite

Both the cellular- and population-level properties of involved neurons are essential for unveiling the learning and memory functions of the brain. To give equal attention to these two aspects, neural sensors based on microelectrode arrays (MEAs) have been in the limelight due to their noninvasive detection and regulation capabilities. Here, we fabricated a neural sensor using carboxylated graphene/3,4-ethylenedioxythiophene:polystyrenesulfonate (cGO/PEDOT:PSS), which is effective in sensing and monitoring neuronal electrophysiological activity in vitro for a long time. The cGO/PEDOT:PSS-modified microelectrodes exhibited a lower electrochemical impedance (7.26 ± 0.29 kΩ), higher charge storage capacity (7.53 ± 0.34 mC/cm2), and improved charge injection (3.11 ± 0.25 mC/cm2). In addition, their performance was maintained after 2 to 4 weeks of long-term cell culture and 50,000 stimulation pulses. During neural network training, the sensors were able to induce learning function in hippocampal neurons through precise electrical stimulation and simultaneously detect changes in neural activity at multiple levels. At the cellular level, not only were three kinds of transient responses to electrical stimulation sensed, but electrical stimulation was also found to affect inhibitory neurons more than excitatory neurons. As for the population level, changes in connectivity and firing synchrony were identified. The cGO/PEDOT:PSS-based neural sensor offers an excellent tool in brain function development and neurological disease treatment.

show abstract

Low impedance and highly transparent microelectrode arrays (MEA) for in vitro neuron electrical activity probing

Cited by 30 publications

References 24 publications

Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks

Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

A Neural Sensor with a Nanocomposite Interface for the Study of Spike Characteristics of Hippocampal Neurons under Learning Training

Contact Info

Product

Resources

About