Low inhibitory effect of ammonia on the nitrogen-fixing activity of a sludge enriched with nitrogen-fixing bacteria

Ospina‐Betancourth, Carolina; Acharya, Kishor; Sanabria, Janeth; Curtis, Thomas P.

doi:10.1016/j.biteb.2021.100655

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…The ammonium ion concentration of the biohybrid system was higher than that of the native cell on the third and the fifth day, and the total ammonium ion concentration of both the native and biohybrid cells decreased on the fifth day. Similarly, more rapid consumption of ammonium ion than nitrogen have been discovered on nitrogen-fixing bacteria in earlier studies 32 (Fig. 3f).…”

Section: Mechanisms Of Light-augmented Metabolic Activitiessupporting

confidence: 76%

Semiconductor augumented valuable chemical photosynthesis fromRhodospirillum rubrumand mechanism study

Wang

Shu-lan

Liang

et al. 2023

Preprint

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Photosynthetic biohybrid systems based on purple bacteria and semiconducting nanomaterials are promising platforms for sustainable solar-powered chemical production. However, these types of biohybrid systems have not been fully developed to date, and their energy utilization and electron transfer mechanisms are not well understood. Herein, a Rhodospirillum rubrum-CdS biohybrid system was successfully constructed. The photosynthetic activity and photoelectrochemical properties of biohybrid system were analyzed. Chromatographic and spectroscopic studies confirmed the metabolic activities of R. rubrum cells were effectively augmented by surface-deposited CdS nanoparticles and validated with increased H2 evolution, polyhydroxybutyric acid (PHB) production, and solid biomass accumulation. Energy consumption and metabolic profiles of R. rubrum-CdS biohybrid system exhibited a growth phase-dependent behaviour. Photoelectrochemical study confirmed that light-excited electrons from CdS enhanced photosynthetic electron flow of R. rubrum cells. Monochromatic light modulated photoexcitation of biohybrid system was utilized to explore interfacial electron transfer between CdS and R. rubrum cells, and the results showed that CdS enhanced the utilization of blue light by R. rubrum cells. This work investigated the feasibility and prospect of utilizing R. rubrum in semi-artificial photosynthesis of valuable products, and offered insights into the energy utilization and the electron transfer mechanism between nanomaterials and purple bacteria.

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Section: Mechanisms Of Light-augmented Metabolic Activitiessupporting

confidence: 76%

Semiconductor augumented valuable chemical photosynthesis fromRhodospirillum rubrumand mechanism study

Wang

Shu-lan

Liang

et al. 2023

Preprint

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“…3f ). Similarly, more rapid consumption of ammonium ions than that of nitrogen has been discovered in nitrogen-fixing bacteria in earlier studies 33 . We attribute these results to the different ways in which the photogenerated electrons produced by CdS are utilized during the different growth phases of bacteria.…”

Section: Mechanisms Of Light-augmented Metabolic Activitiesmentioning

confidence: 52%

Semiconductor augmented hydrogen and polyhydroxybutyrate photosynthesis from Rhodospirillum rubrum and a mechanism study

Wang,

Shi,

Liang

et al. 2023

Green Chem.

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“…This modified enzyme then attaches to the nifR region of the nifRLA operon, leading to the inhibition of nifL and nifA gene transcription. Consequently, σ-RNA polymerase is unable to initiate the transcription of other genes [31]. Under conditions of limited nitrogen availability during growth, the inhibitory effect of nifL protein on the nifA protein is counteracted by the Glnk protein's antagonistic action on the nifL proteins [32].…”

Section: Nifrla Operonmentioning

confidence: 99%

Nitrogen Fixation by Rhizobacterial Nif Mechanism: An Advanced Genetic Perspective

Idris Usman,

Muazu Wali

2024

Updates on Rhizobacteria

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The global population’s rising nutritional needs pose a challenge, demanding a 70% boost in agricultural efficiency to feed 10 billion people by 2050. This task is complicated by limited arable land and the imperative to reduce agrochemical usage. To overcome this, harnessing rhizobacteria and comprehending nif gene mechanisms to enhance nitrogen fixation is crucial. Nif genes encode enzymes, converting atmospheric nitrogen into vital ammonia found in diverse prokaryotes. Nitrogen-fixing bacteria, categorized as autogenous, symbiotic, and combined, collaborate with plants or independently fix nitrogen. Nitrogenase enzymes, represented by Mo, V, and Fe forms, enable this conversion. Nif operons, like nifRLA, nifHDK, nifENB, nifJ, nifUSVM, and nifWF, are pivotal in nitrogen fixation, synthesizing components, and regulating enzymes. Biotech advancements, like 2A peptides and gene manipulation, show promise in boosting crop yields. Translating rhizobacterial nitrogen fixation to cereals could revolutionize agriculture and global food security.

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Low inhibitory effect of ammonia on the nitrogen-fixing activity of a sludge enriched with nitrogen-fixing bacteria

Cited by 5 publications

References 41 publications

Semiconductor augumented valuable chemical photosynthesis fromRhodospirillum rubrumand mechanism study

Semiconductor augumented valuable chemical photosynthesis fromRhodospirillum rubrumand mechanism study

Semiconductor augmented hydrogen and polyhydroxybutyrate photosynthesis from Rhodospirillum rubrum and a mechanism study

Nitrogen Fixation by Rhizobacterial Nif Mechanism: An Advanced Genetic Perspective

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