Low intensity infrared laser effects on Escherichia coli cultures and plasmid DNA

Fonseca, A S; Teixeira, Adilson Fonseca; Presta, G.; Geller, Mauro; Paoli, Flávia de

doi:10.1134/s1054660x12100076

Cited by 18 publications

(28 citation statements)

References 47 publications

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“…However, consecutive dichromatic laser radiation induces filamentation percentages similar to those with monochromatic infrared laser in stationary E. coli AB1157 cultures (figure 6). This suggests that dichromatic laser radiation effects are dependent on the physiological condition of cells, as suggested in other studies of monochromatic laser radiation [21,23,24]. Induction of a filamentation phenotype in E. coli AB1157 exposed to monochromatic red or infrared laser in this study is similar to previous studies [24,28,30,32].…”

Section: Discussionsupporting

confidence: 90%

“…Free radicals act as second messengers in specific cell signaling processes and alter genetic expression [20,21]. However, laser-induced free radicals may also react with a number of intracellular macromolecules (DNA, for example), altering their functions and inducing adverse effects at sub-lethal level [22][23][24]. For this, laser radiation parameters (dose, power and emission mode) must be adjusted to ensure the safety and effectiveness of therapeutic applications of low-intensity lasers in monochromatic and dichromatic light irradiation schedules.…”

Section: Dichromatic Laser Radiation Effects On Dna Of Escherichia Co...mentioning

confidence: 99%

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Dichromatic laser radiation effects on DNA ofEscherichia coliand plasmids

et al. 2015

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Dichromatic and consecutive laser radiations have attracted increased attention for clinical applications as offering new tools for the treatment of dysfunctional tissues in situations where monochromatic radiation is not effective. This work evaluated the survival, filamentation and morphology of Escherichia coli cells, and the induction of DNA lesions, in plasmid DNA exposed to low-intensity consecutive dichromatic laser radiation. Exponential and stationary wild type and formamidopyrimidine DNA glycosylase/MutM protein deficient E. coli cultures were exposed to consecutive low-intensity dichromatic laser radiation (infrared laser immediately after red laser) to study the survival, filamentation and morphology of bacterial cells. Plasmid DNA samples were exposed to dichromatic radiation to study DNA lesions by electrophoretic profile. Dichromatic laser radiation affects the survival, filamentation and morphology of E. coli cultures depending on the growth phase and the functional repair mechanism of oxidizing lesions in DNA, but does not induce single/double strands breaks or alkali-labile DNA lesions. Results show that low-intensity consecutive dichromatic laser radiation induces biological effects that differ from those induced by monochromatic laser radiation, suggesting that other therapeutic effects could be obtained using dichromatic radiation.

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Section: Discussionsupporting

confidence: 90%

Section: Dichromatic Laser Radiation Effects On Dna Of Escherichia Co...mentioning

confidence: 99%

Dichromatic laser radiation effects on DNA ofEscherichia coliand plasmids

et al. 2015

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“…It is possible that low‐level lasers in pulsed emission mode at high frequencies are capable of inducing free radical production at greater levels than in continuous wave. In fact, pre‐exposure to low‐level red laser in pulsed emission mode induces larger protection against hydrogen peroxide than continuous wave and laser‐induced lipid peroxidation in blood cells is larger in pulsed emission mode than in continuous wave .…”

Section: Discussionmentioning

confidence: 99%

“…The laser radiation energy is transformed in a primary photosignal and in sequence it is transduced and amplified into cells . It is thought that, in this transduction process, there is production of free radicals , and these reactive species could react with intracellular molecules, including DNA and membrane lipids .…”

Section: Introductionmentioning

confidence: 99%

DNA damage in blood cells exposed to low‐level lasers

et al. 2015

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“…Free radicals act as second messengers in specific cell signaling processes and alter gene expression [9]. However, laser-induced free radicals could react with a number of intracellular macromolecules (DNA, for example) altering their functions at sub-lethal level [10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

et al. 2016

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Lasers emit light beams with specific characteristics, in which wavelength, frequency, power, fluence, and emission mode properties determine the photophysical, photochemical, and photobiological responses. Low-intensity lasers could induce free radical generation in biological tissues and cause alterations in macromolecules, such as DNA. Thus, the aim of this work was to evaluate excision repair cross-complementing group 1 (ERCC1) and excision repair cross-complementing group 2 (ERCC2) messenger RNA (mRNA) expression in biological tissues exposed to low-intensity lasers. Wistar rat (n = 28, 4 for each group) skin and muscle were exposed to low-intensity red (660 nm) and near-infrared (880 nm) lasers at different fluences (25, 50, and 100 J/cm(2)), and samples of these tissues were withdrawn for RNA extraction, cDNA synthesis, and gene expression evaluation by quantitative polymerase chain reaction. Laser exposure was in continuous wave and power of 100 mW. Data show that ERCC1 and ERCC2 mRNA expressions decrease in skin (p < 0.001) exposed to near-infrared laser, but increase in muscle tissue (p < 0.001). ERCC1 mRNA expression does not alter (p > 0.05), but ERCC2 mRNA expression decreases in skin (p < 0.001) and increases in muscle tissue (p < 0.001) exposed to red laser. Our results show that ERCC1 and ERCC2 mRNA expression is differently altered in skin and muscle tissue exposed to low-intensity lasers depending on wavelengths and fluences used in therapeutic protocols.

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Low intensity infrared laser effects on Escherichia coli cultures and plasmid DNA

Cited by 18 publications

References 47 publications

Dichromatic laser radiation effects on DNA ofEscherichia coliand plasmids

Dichromatic laser radiation effects on DNA ofEscherichia coliand plasmids

DNA damage in blood cells exposed to low‐level lasers

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

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