Low-Intensity Pulsed Ultrasound Attenuates Periodontal Ligament Cells Apoptosis by Activating Yes-Associated Protein-Regulated Autophagy

Jian, Zixiang; Li, Yao; Zhang, Chuangwei; Zhong, Wenjie; Ai, Dongqing; He, Yao; Song, Jinlin

doi:10.1016/j.ultrasmedbio.2023.01.008

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…of YAP expression enhanced apoptosis and the of autophagy in LPS-treated periodontal ligament cells [84]. Another study also suggested that LIPUS could delay the progression of osteoarthritis by restoring autophagy in chondrocytes via YAP-RIPK1-NF-κB axis.…”

Section: Lipus and Yapmentioning

confidence: 90%

The roles of Hippo/YAP signaling pathway in physical therapy

Pan,

Hao,

Deng

et al. 2024

Cell Death Discov.

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Cellular behavior is regulated by mechanical signals within the cellular microenvironment. Additionally, changes of temperature, blood flow, and muscle contraction also affect cellular state and the development of diseases. In clinical practice, physical therapy techniques such as ultrasound, vibration, exercise, cold therapy, and hyperthermia are commonly employed to alleviate pain and treat diseases. However, the molecular mechanism about how these physiotherapy methods stimulate local tissues and control gene expression remains unknow. Fortunately, the discovery of YAP filled this gap, which has been reported has the ability to sense and convert a wide variety of mechanical signals into cell-specific programs for transcription, thereby offering a fresh perspective on the mechanisms by which physiotherapy treat different diseases. This review examines the involvement of Hippo/YAP signaling pathway in various diseases and its role in different physical therapy approaches on diseases. Furthermore, we explore the potential therapeutic implications of the Hippo/YAP signaling pathway and address the limitations and controversies surrounding its application in physiotherapy.

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Section: Lipus and Yapmentioning

confidence: 90%

The roles of Hippo/YAP signaling pathway in physical therapy

Pan,

Hao,

Deng

et al. 2024

Cell Death Discov.

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“…The harvested DP T lymphocytes were transferred to lymphoid differentiation material-coated plates, and 10 ng/mL IL-2, 10 ng/mL IL-15, and 6.25 µL human CD3/CD28/CD2 T cell activator were added to each well for 7–14 days for maturation and differentiation of CD8 + T cells ( Minagawa et al, 2018 ; Hu et al, 2020 ; Ji et al, 2022 ; Wu et al, 2022 ; Jian et al, 2023 ). Except for mature T cells, most immature lymphocytes, including CD4 + CD8 + DP T cells, died as a result of T cell receptor (TCR) rearrangement.…”

Section: Methodsmentioning

confidence: 99%

“…Maintaining the standard manufacturer protocol, the cell cycle in Raji and A549 cells was also analyzed using flow cytometry. ( Jian et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy

Chen,

Huang,

Niu

et al. 2023

Front. Bioeng. Biotechnol.

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Background: Chimeric antigen receptor (CAR) T cell treatment involves in vitro production of T cells from patient blood with synthetic receptors specific to a cancer antigen. They circumvent the major histocompatibility complex to recognize the tumor antigen, reducing hematologic malignancy remission rates by 80%. Considering the efficacy of CAR-T treatment, the present work aimed at generating functional clusters of differentiation (CD)8 + T cells from human induced pluripotent stem cells (hiPSC) and to generate hiPS-CAR-T cells with high antigen-specific cytotoxicity.Methods: The Alkaline phosphatase assay and MycoEasy rapid mycoplasma detection kit was implemented for detection of hiPSCs and mycoplasma, respectively. The CD34+ HSPCs were harvested in AggreWellTM 400 using a 37-micron reversible strainer. Likewise, the lymphoid progenitor and CD4+CD8+ DP T cells were also harvested. The Cell Counting Kit-8 (CCK-8) assay was used to mark cytotoxicity and ELISA was used to detect IFN-γ secretion. Further, flow cytometry and transwell chambers were used to assess cell cycle, and migration and invasion. Finally, the in vivo antitumor effects of the CAR-T cells were evaluated using experimental animals (mice).Results: Results revealed that a serum-free, feeder layer-free differentiation system significantly yielded hiPSC-based T cell immunotherapy with interleukin-2, interleukin-15, and activators at the differentiation stage to promote the maturation of these cells into human induced pluripotent stem (hiPS)-T cells. The infection of hiPSCs with the CD19 CAR lentivirus resulted in the production of the hiPSC-CAR-T cells. We validated the function of hiPS-CAR-T cells in vivo and in vitro experimentation which revealed no significant differences in cell morphology and function between hiPSC-derived hiPS-CAR-T cells and peripheral blood-derived CAR-T cells.Conclusion: This study developed a culture method that is efficient and clinically useful to make functional CD8+ T cells from hiPSC and to get hiPS-CAR-T cells with high antigen-specific cytotoxicity that are not very different from CAR T cells found in peripheral blood. As a result, our findings may open the way for the clinical use of hiPSC to create functional CD8+ T and hiPS-CAR-T cells cells for use in cell-based cancer therapy.

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“…Furthermore, Wang et al demonstrated that TAZ served as an upstream factor responsible for activating Wnt/β-catenin signaling during cyclic tensile stress–mediated osteogenic differentiation of PDLCs ( Wang Y. et al, 2020 ). Jian et al established a lipopolysaccharide (LPS)–induced periodontal ligament inflammatory environment and discovered that the mechanical signal transformed by low-intensity pulsed ultrasound activated the nuclear expression of YAP ( Jian et al, 2023 ). This was accompanied by reduced alveolar bone resorption.…”

Section: Yap and Taz Regulate Periodontal Homeostasis And Remodelingmentioning

confidence: 99%

Distinct and overlapping functions of YAP and TAZ in tooth development and periodontal homeostasis

Ma,

Fan,

Geng

2024

Front. Cell Dev. Biol.

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Orthodontic tooth movement (OTM) involves mechanical–biochemical signal transduction, which results in tissue remodeling of the tooth–periodontium complex and the movement of orthodontic teeth. The dynamic regulation of osteogenesis and osteoclastogenesis serves as the biological basis for remodeling of the periodontium, and more importantly, the prerequisite for establishing periodontal homeostasis. Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are key effectors of the Hippo signaling pathway, which actively respond to mechanical stimuli during tooth movement. Specifically, they participate in translating mechanical into biochemical signals, thereby regulating periodontal homeostasis, periodontal remodeling, and tooth development. YAP and TAZ have widely been considered as key factors to prevent dental dysplasia, accelerate orthodontic tooth movement, and shorten treatment time. In this review, we summarize the functions of YAP and TAZ in regulating tooth development and periodontal remodeling, with the aim to gain a better understanding of their mechanisms of action and provide insights into maintaining proper tooth development and establishing a healthy periodontal and alveolar bone environment. Our findings offer novel perspectives and directions for targeted clinical treatments. Moreover, considering the similarities and differences in the development, structure, and physiology between YAP and TAZ, these molecules may exhibit functional variations in specific regulatory processes. Hence, we pay special attention to their distinct roles in specific regulatory functions to gain a comprehensive and profound understanding of their contributions.

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Low-Intensity Pulsed Ultrasound Attenuates Periodontal Ligament Cells Apoptosis by Activating Yes-Associated Protein-Regulated Autophagy

Cited by 10 publications

References 38 publications

The roles of Hippo/YAP signaling pathway in physical therapy

The roles of Hippo/YAP signaling pathway in physical therapy

Regeneration of T cells from human-induced pluripotent stem cells for CAR-T cell medicated immunotherapy

Distinct and overlapping functions of YAP and TAZ in tooth development and periodontal homeostasis

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