Low-Intensity Pulsed Ultrasound Produced an Increase of Osteogenic Genes Expression During the Process of Bone Healing in Rats

Focused Low-Intensity Pulsed Ultrasound Enhances Bone Regeneration in Rat Calvarial Bone Defect through Enhancement of Cell Proliferation

“…HIPUS has been used in research on prostate cancer (Blana et al 2004;Pfeiffer et al 2012) and hepatocellular carcinoma (Cheung et al 2013a;Ni et al 2012). In a previous study, LIPUS was found to stimulate new bone formation by inducing the expression of several proteins (Gleizal et al 2006;Reher et al 1997) and genes (F avaro-P ıpi et al 2010;Harle et al 2001). In most studies conducted to date, LIPUS has been used to induce the regeneration of defects in long bones such as the femur and fibula, and the results indicate that it results in a significant increase in bone growth (Claes and Wilie 2007;Duarte 1983;Tsai et al 1992).…”

Low Intensity Pulsed Ultrasound Enhanced Mesenchymal Stem Cell Recruitment through Stromal Derived Factor-1 Signaling in Fracture Healing

“…Taken together, these data suggest that SDF-1/CXCR4 signaling plays a very important role in fracture healing. Disrupting the SDF/CXCR4 pathway during LIPUS treatment could partially reduce, but not fully abolish LIPUS-induced fracture healing, indicating that other factors or signaling pathways might also be involved in this accelerated fracture repair process, such as increased blood circulation [88], upregulated osteogenic genes [89], and gap junctional cell-to-cell intercellular communication in rat MSCs [69].…”

Low-intensity pulsed ultrasound enhances bone morphogenetic protein expression of human mandibular fracture haematoma-derived cells

“…2 Fávaro-Pípi et al found that LIPUS stimulation in rats significantly increased BMP-4 expression on days 7 and 25. 9 10,23 In this study using MHCs extracted from human mandibular fracture sites, only BMP-4 expression was increased in the absence of LIPUS application on days 4 and 8 (Fig. 1).…”

“…7 Several in vitro investigations of animal cells suggest that LIPUS stimulation also increases BMP expression. 2,8,9 Moreover, evidence suggests that LIPUS induces osteogenic differentiation of human periodontal ligament cells by activating BMP-2 signaling. 10 However, how the LIPUS, which is mainly used to accelerate fracture healing, regulates BMP expression in humans has not been fully elucidated, especially in the case of human mandibular fractures.…”