Low‐intensity ultrasound and microbubbles enhance the antitumor effect of cisplatin

Watanabe, Yukiko; Aoi, Atsuko; Horie, Seichi; Tomita, Noriko; Mori, Shiro; Morikawa, Hidehiro; Matsumura, Yasuhiro; Vassaux, Georges; Kodama, Tetsuya

doi:10.1111/j.1349-7006.2008.00989.x

Cited by 76 publications

(77 citation statements)

References 49 publications

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“…Microbubbles can also convert energy of the ultrasonic vibration into a local thermal therapeutic effect [22]. Streaming effect on microbubbles in ultrasonic field can destroy cells [23] and can cause permeabilisation of membranes for drugs [24] in particular the anti-cancer ones [25,26]. Microbubbles of suitable composition possess ability to target specific cellular markers of disease and deliver localised chemotherapy.…”

Section: Biomedical Usesmentioning

confidence: 99%

What can be done with microbubbles generated by a fluidic oscillator? (survey)

Tesař

2017

EPJ Web Conf.

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Abstract.Recently discovered possibility of generating sub-millimetre bubbles by means of pulsating the inlet gas flow by a fluidic oscillator has opened a way to many interesting and advantageous engineering applications. Some of them have been known before, but were too inefficient and nearly forgotten. Now they deserve discussing in the new perspective. Other, applications, new ones, appear now in literature at a rapid pace. This paper, by providing a survey of already existing possibilities and advantages, aims at providing the readers an inspiration for their own developments.

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Section: Biomedical Usesmentioning

confidence: 99%

What can be done with microbubbles generated by a fluidic oscillator? (survey)

Tesař

2017

EPJ Web Conf.

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“…Signal generation and pressure measurements were as previously described (5,6). After the legs of the mice were shaved, the tibialis anterior (TA) muscle was immersed in water and exposed to ultrasound.…”

Section: Ultrasound Conditions and Transfection Methodsmentioning

confidence: 99%

Delivery of Na/I Symporter Gene into Skeletal Muscle Using Nanobubbles and Ultrasound: Visualization of Gene Expression by PET

Watanabe¹,

Horie²,

Funaki³

et al. 2010

J Nucl Med

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The development of nonviral gene delivery systems is essential in gene therapy, and the use of a minimally invasive imaging methodology can provide important clinical endpoints. In the current study, we present a new methodology for gene therapy-a delivery system using nanobubbles and ultrasound as a nonviral gene delivery method. We assessed whether the gene transfer allowed by this methodology was detectable by PET and bioluminescence imaging. Methods: Two kinds of reported vectors (luciferase and human Na/I symporter [hNIS]) were transfected or cotransfected into the skeletal muscles of normal mice (BALB/c) using the ultrasound-nanobubbles method. The kinetics of luciferase gene expression were analyzed in vivo using bioluminescence imaging. At the peak of gene transfer, PET of hNIS expression was performed using our recently developed PET scanner, after 124 I injection. The imaging data were confirmed using reverse-transcriptase polymerase chain reaction amplification, biodistribution, and a blocking study. The imaging potential of the 2 methodologies was evaluated in 2 mouse models of human pathology (McH/lpr-RA1 mice showing vascular disease and C57BL/10-mdx Jic mice showing muscular dystrophy). Results: Peak luciferase gene activity was observed in the skeletal muscle 4 d after transfection. On day 2 after hNIS and luciferase cotransfection, the expression of these genes was confirmed by reverse-transcriptase polymerase chain reaction on a muscle biopsy. PET of the hNIS gene, biodistribution, the blocking study, and autoradiography were performed on day 4 after transfection, and it was indicated that hNIS expression was restricted to the site of plasmid administration (skeletal muscle). Similar localized PET and 124 I accumulation were successfully obtained in the diseasemodel mice. Conclusion: The hNIS gene was delivered into the skeletal muscle of healthy and disease-model mice by the ultrasound-nanobubbles method, and gene expression was successfully visualized with PET. The combination of ultrasound-nanobubble gene transfer and PET may be applied to gene therapy clinical protocols.

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“…Microbubbles can convert energy of ultrasonic vibration into a local thermal therapeutic effect (Kanagawa, 2013). Streaming effect on microbubbles in ultrasonic field can destroy cells (Kooiman et al, 2011) and can cause their outer membranes to become permeable for drugs (Oh et al, 2014) particularly anticancer ones (Watanabe et al, 2008). Microbubbles are also used in measuring absolute blood pressure (Tremblay-Darveau et al, 2014).…”

Section: Applications and Importance Of Microbubblesmentioning

confidence: 99%

Fluidic Generator Of Microbubbles – Oscillator With Gas Flow Reversal For A Part Of Period

Tesař

2015

Acta Mechanica Et Automatica

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Paper presents a fluidic device developed for generation of small (less than 1 mm in diameter) microbubbles in a liquid from gas passing gas through small passages. Until now the bubbles are larger than the size of aerator passage exits so that making the passages smaller did not result in obtaining the desirable microbubbles. Analysis of high-speed camera images (obtained with a special lens of large working distance) have shown show that the large bubble size is caused by slow ascent motion of very small bubbles so that they get into mutual contact and grow by conjunction. The solution is to pulsate the supplied gas flow by a no-moving-part fluidic oscillator. The generated small bubble is moved back into the aerator passage where it is for a part of oscillation period protected from the conjunction with other, previously generated microbubbles.

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Low‐intensity ultrasound and microbubbles enhance the antitumor effect of cisplatin

Abstract: Cell permeabilization using microbubbles (MB) and low-intensity ultrasound (US

Cited by 76 publications

References 49 publications

What can be done with microbubbles generated by a fluidic oscillator? (survey)

What can be done with microbubbles generated by a fluidic oscillator? (survey)

Delivery of Na/I Symporter Gene into Skeletal Muscle Using Nanobubbles and Ultrasound: Visualization of Gene Expression by PET

Fluidic Generator Of Microbubbles – Oscillator With Gas Flow Reversal For A Part Of Period

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