2016
DOI: 10.2116/analsci.32.135
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Low-interferences Determination of the Antioxidant Capacity in Fruits Juices Based on Xanthine Oxidase and Mediated Amperometric Measurements in the Reduction Mode

Abstract: A low-interferences enzymatic sensor for evaluating the antioxidant capacity was developed. Xanthine oxidase was used to produce superoxide radicals that spontaneously dismutate to hydrogen peroxide. Low xanthine concentrations were used to minimize the rapid dismutation of the superoxide radical before its fast reaction with antioxidants. The sensor operates in the reduction mode, and evaluations with low interferences of the antioxidant capacity are based on the detection of remaining hydrogen peroxide using… Show more

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Cited by 3 publications
(2 citation statements)
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“…There are numerous electrodes modified with various electrochemical mediators reported for GSH detection. 23 Prussian blue is a mediator suitable for food analysis 24 that was reported to have a good sensitivity for the detection of GSH. 25 Thorough electrochemical studies have demonstrate that Prussian blue is an effective mediator for several thiolic compounds such as cysteamine or thiocholine, but ineffective for GSH.…”
Section: Resultsmentioning
confidence: 99%
“…There are numerous electrodes modified with various electrochemical mediators reported for GSH detection. 23 Prussian blue is a mediator suitable for food analysis 24 that was reported to have a good sensitivity for the detection of GSH. 25 Thorough electrochemical studies have demonstrate that Prussian blue is an effective mediator for several thiolic compounds such as cysteamine or thiocholine, but ineffective for GSH.…”
Section: Resultsmentioning
confidence: 99%
“…A chronoamperometric study was performed by Brainina et al [19], where the total antioxidant activity was correlated to the oxidation current of a mediator (potassium ferrocyanide K 4 [Fe(CN) 6 ]) which is formed in the reduction of potassium ferricyanide (K 3 [Fe(CN) 6 ]) in the presence of AOx. Another amperometric xanthine oxidase (XOD)- based sensor was developed by Bucur et al [20]. The quantification of the analytical signal was based on the detection of H 2 O 2 , where xanthine and ascorbic acid were considered antioxidants.…”
Section: Introductionmentioning
confidence: 99%