Low-ionic-strength saline solution–antiglobulin test (LISS-AGT)

Abstract

The use of low-ionic-strength saline (LISS) solution as an enhancement for antibody screening and crossmatching was first described by Löw and Messeter in 1974. This method allowed for a reduced incubation time while maintaining adequate specificity and sensitivity of the antiglobulin test (AGT). Since then, the LISS-AGT tube method has been widely used in antibody detection and identification, as well as compatibility testing. As initially described, the method used red blood cells suspended in LISS. Modifications of the method led to development of the commercially prepared LISS additive solutions in use today. The LISS-AGT can be used effectively to detect alloantibodies of all major blood groups in antibody detection, antibody identification, and crossmatching procedures. Immunohematology 2018; 34:57-60.

Pengaruh Suhu Dan Lama Inkubasi Pemeriksaan Crossmatch Metode Gel Test Pada Spesimen Darah Pasien Talasemia

“…Menurut Hughes-Jones tingkat penyerapan antibodi meningkat 1000 kali ketika menggunakan larutan dengan kekuatan ionik rendah, hal ini mengurangi muatan listrik positif yang mengelilingi sel darah merah bermuatan negatif yang memungkinkan antibodi bekerja lebih efisien untuk menggumpalkan sel darah merah yang tidak kompatibel. 16…”