Low level 809‐nm diode laser‐induced in vitro stimulation of the proliferation of human gingival fibroblasts

Kreisler, Matthias; Christoffers, Ann B.; Al-Haj, Haitham; Willershausen, Brita; dʼHoedt, B

doi:10.1002/lsm.10060

Cited by 197 publications

(133 citation statements)

References 30 publications

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“…Eduardo et al 12 verificaram uma maior atividade proliferativa de célu-las tronco da polpa dental humana submetidas a irradiação laser (InGaAlP) de 660nm e 20mW em relação ao controle não irradiado nas mesmas condições de nutrição. Enquanto que Kreisler et al 13 investigaram os efeitos da irradiação laser na proliferação de fibroblastos gengivais humanos. As células foram irradiadas com dose de 1.96-7.84J/cm 2 , usando um laser semicondutor de 809nm e 10mW.…”

Section: Métodos Métodos Métodos Métodos Métodosunclassified

“…Numerosos estudos experimentais in vitro já foram realizados 7,10,12,13,31,26,27,28 concordando com a hipótese de que a laserterapia seja capaz de influenciar os processos metabólicos celulares, ao aumentar a proliferação de várias células, como os fibroblastos, osteoblastos, queratinócitos, células tronco e células oriundas de tumores malignos.…”

Section: Discussão Discussão Discussão Discussão Discussãounclassified

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Ação da laserterapia no processo de proliferação e diferenciação celular: revisão da literatura

Henriques

Cazal

Castro

2010

Rev. Col. Bras. Cir.

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O uso da luz laser de baixa intensidade vem sendo utilizado como terapia coadjuvante ou de forma terapêutica isolada em várias especialidades odontológicas. Suas principais indicações incluem ação anti-inflamatória, analgésica e indutora da reparação tecidual. O poder cicatrizante do laser de baixa potência é discutido neste trabalho assim como os mecanismos de biomodulação e estimulação da mitose. Estas propriedades, já estudadas em células benignas, quando aplicadas em células neoplásicas malignas, abrem espaço para discussões. O objetivo do presente trabalho foi realizar uma revisão da literatura sobre os aspectos indutivos do laser no processo de proliferação celular principalmente no que se refere a estes mecanismos em células neoplásicas malignas.

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Section: Métodos Métodos Métodos Métodos Métodosunclassified

Section: Discussão Discussão Discussão Discussão Discussãounclassified

Ação da laserterapia no processo de proliferação e diferenciação celular: revisão da literatura

Henriques

Cazal

Castro

2010

Rev. Col. Bras. Cir.

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“…For example, LEVL increases the rate of wound healing (3), enhances the fertilizing capability of sperm cells (4), and increases the rate of healing bone defects (5). In vitro studies have found that LEVL increases proliferation of cells as fibroblasts (6), keratinocytes (7), and lymphocytes (8) and induces the respiratory burst in neutrophils (9). The mechanism of photobiostimulation by LEVL is still unclear.…”

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Low Energy Visible Light Induces Reactive Oxygen Species Generation and Stimulates an Increase of Intracellular Calcium Concentration in Cardiac Cells

Lavi

Shainberg

Friedmann

et al. 2003

Journal of Biological Chemistry

168

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Low energy visible light (LEVL) irradiation has beenshown to exert some beneficial effects on various cell cultures. For example, it increases the fertilizing capability of sperm cells, promotes cell proliferation, induces sprouting of neurons, and more. To learn about the mechanism of photobiostimulation, we studied the relationship between increased intracellular calcium ( Life on earth is entirely dependent upon the interaction of sunlight with cells especially in plant photosynthesis (1). Sunlight also has medical benefits, which have been exploited for over thousands of years in ancient Egypt, India, and China in treating skin diseases, psoriasis, vitiligo, and even cancer (2). Recent observations show that even low energy visible light (LEVL) 1 can serve as a medical tool. For example, LEVL increases the rate of wound healing (3), enhances the fertilizing capability of sperm cells (4), and increases the rate of healing bone defects (5). In vitro studies have found that LEVL increases proliferation of cells as fibroblasts (6), keratinocytes (7), and lymphocytes (8) and induces the respiratory burst in neutrophils (9). The mechanism of photobiostimulation by LEVL is still unclear. It has been suggested that reactive oxygen species (ROS), which can be produced by photosensitization of endogenous cell chromophores such as cytochromes (10) [Ca 2ϩ ] i followed by myosin phosphorylation and cell contractions (26). Growth factors and hormones were shown to stimulate ROS production, which were dependent on [Ca 2ϩ ] i rise (27). The relationship between ROS and [Ca 2ϩ ] i has been suggested to involve the redox-sensitive transcription factor N␤, which was found to change [Ca 2ϩ ] i homeostasis in response to changes in the redox state of thiol groups (28). The kinetics that characterize the [Ca 2ϩ ] i elevation have been shown to be an important parameter determining the kind of signal that will be evoked. Livingston et al. (29) showed that high concentrations of oxidants (Ͼ50 M) caused a sustained increase in [Ca 2ϩ ] i , whereas a transient increase in [Ca 2ϩ ] i was observed following administration of a low concentration of oxidants. More than a 4-fold increase in the [Ca 2ϩ ] i level was obtained in photodynamic treatment of mouse myeloma cells that had been enriched with exogenous photosensitizers before illumination, whereas only a slight increase in [Ca 2ϩ ] i was observed in irradiated cells without exogenous photosensitizers (30).Only a few works have dealt with calcium/ROS changes following LEVL irradiation. In this study, we have investigated * The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.§ The article describes research performed by Ronit Lavi as partial fulfillment of the requirements for her Ph.D. degree at Bar-Ilan University. To whom correspondence should be addressed. Tel.: 972-3-5317797; Fax: 972-3-...

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“…Providing laser treatment at low energy levels can be useful and beneficial for healing and regeneration, the predominance of literature suggests that LLLT occurs with visible and near-infrared wavelengths from 633 to 904nm [36][37][38]. Diode laser would be better for biostimulation due to less wavelength than the Er:YAG Laser.…”

Section: Discussionmentioning

confidence: 99%

Multiple Applications with Er:YAG Laser in Apicoectomy - A Clinical Case Report

Yu¹

2017

Dentistry

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The setting was 50 mJ/10 Hz with 100% of water for full mouth laser scaling and then ultrasonic scaling tip was used for supragingival scaling. In addition, another laser setting was applied at 50 mJ/30Hz with 100% of water for subgingival sterilization and then ultrasonic round tip was used for subgingival curettage [7,8]. Step 2. Vertical soft tissue incisionThe setting was 100 mJ/40 Hz with 100% of water for incision [in vestibular near apex of #21] (Figure 3). Step 3. Granulation tissues ablationThe setting was 400 mJ/15Hz with 100% of water. Granulation tissues were ablation thoroughly (Figures 4 and 5).

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Low level 809‐nm diode laser‐induced in vitro stimulation of the proliferation of human gingival fibroblasts

Abstract: A cellular effect of the soft laser irradiation on HGF is evident. Its duration, however, seems to be limited. These findings might be clinically relevant, indicating that repeated treatments are necessary to achieve a positive laser effect in clinical applications.

Cited by 197 publications

References 30 publications

Ação da laserterapia no processo de proliferação e diferenciação celular: revisão da literatura

Ação da laserterapia no processo de proliferação e diferenciação celular: revisão da literatura

Low Energy Visible Light Induces Reactive Oxygen Species Generation and Stimulates an Increase of Intracellular Calcium Concentration in Cardiac Cells

Multiple Applications with Er:YAG Laser in Apicoectomy - A Clinical Case Report

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