Low-level counting by liquid scintillation—II

Lieberman, R.; Moghissi, A.A.

doi:10.1016/0020-708x(70)90143-2

Cited by 41 publications

(3 citation statements)

References 12 publications

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“…Radioactivity was measured with a Beckman LS-1OOC liquid scintillation spectrometer. An efficiency of 52% was observed for 1 -mL aqueous samples dispersed in 10 mL of a toluene-Triton X-100 solution (Lieberman & Moghissi, 1970). Elemental analysis was performed by Atlantic Microlab, Atlanta, Ga. Infrared spectra were performed on a Perkin-Elmer 457 instrument.…”

mentioning

confidence: 99%

Active site directed irreversible inhibition of thermolysin

Rasnick¹,

Powers²

1978

Biochemistry

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1 9 7 8 4363 in solution is characterized by a dynamic equilibrium of multiple conformational states. Detailed examination of the kinetic and equilibrium properties of these states has and will continue to provide new insights into the mechanism of action.ABSTRACT: Thermolysin is irreversibly inhibited at pH 7.2 by CICH~CO-DL-(N-OH)L~U-OCH~ and 2-(N-bromoacetyl-N-hydroxyamino)-4-methylpentanonitrile. The inhibition reactions exhibit saturation kinetics with K I values of 7.5 and 0.80 mM, respectively. Competitive inhibitors of thermolysin (P-Leu-Trp-OK and Z-Phe-OH) hindered the alkylation. The stoichiometry of the reaction was demonstrated to be 0.97 to 1 by use of I4C-labeled CICH~CO-DL-(N-OH)L~U-OCH~. No change was observed in the amino acid analysis of the alkylated thermolysin. The inhibitor moiety could be removed with 1 m M NaOH or 1 M NH20H, observations which support the existence of an ester linkage between the enzyme and inhibitor. Degradation of thermolysin alkylated with I4Clabeled CICH~CO-DL-(N-OH)L~U-OCH~ with CNBr demonstrated that the FI fragment (residues 121 -205) contained

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mentioning

confidence: 99%

Active site directed irreversible inhibition of thermolysin

Rasnick¹,

Powers²

1978

Biochemistry

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“…Fractions (200 µ ) of eluate were taken into glass vials of nominal capacity 3.7 mL and diluted to 400 µ by addition of water before 3.4 mL of scintillation fluid was added. The latter was composed of Triton N-101 /xylene (Lieberman & Moghissi, 1970) plus fluors, to which 4 mL of 12 N HC1 had been added per liter. Radioactivity was determined in an ambient temperature scintillation counter.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of ¹²⁵I labeled N-3-(4-hydroxyphenyl)propionyl aminoacyl transfer ribonucleic acids

Tener¹,

Delaney²,

Grigliatti³

et al. 1978

Biochemistry

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A method for the isolation and labeling to high specific radioactivity of individual isoaccepting tRNAs is described. After blocking reactive minor bases by acetylation and iodination of the crude tRNA, a single family of isoacceptors was aminoacylated. Individual isoacceptors were separated by chromatography on RPC-5 and then acylated with the 3-(4-hydroxyphenyl)propionyl ester of N-hydroxysuccinimide. The product was purified by chromatography on BD-cellulose and RPC-5. This derivatized tRNA was then iodinated with 125I- and Chloramine-T to give a product containing between 5 X 10(7) and 3 X 10(8) dpm/microgram. The suitability of such labeled tRNAs for hybridization to homologous DNA in solution and cytological preparations of chromosomes is discussed with particular reference to Drosophila melanogaster.

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“…Despite this, the use of an aqueous tritium, toluene and Trition-X100 cocktail has been reported frequently [72]- [74], [75], [76]. Further, the sensitivity of the detection systems using Triton surfactants was shown by Lieberman and Moghissi (1970), achieving a sensitivity of 7.4 Bq L -1 of tritium in water [77]. However, since 4 th January 2021 the use of Triton-X100 within the European Union has been limited due to its hazard to human health [78].…”

Section: Liquid Scintillation Cocktailsmentioning

confidence: 99%

Radiometric techniques for the detection and assessment of tritium in aqueous media - a review

Parker

Aspinall

Boxall

et al. 2023

Progress in Nuclear Energy

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Low-level counting by liquid scintillation—II

Cited by 41 publications

References 12 publications

Active site directed irreversible inhibition of thermolysin

Active site directed irreversible inhibition of thermolysin

Synthesis of ¹²⁵I labeled N-3-(4-hydroxyphenyl)propionyl aminoacyl transfer ribonucleic acids

Radiometric techniques for the detection and assessment of tritium in aqueous media - a review

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Low-level counting by liquid scintillation—II

Cited by 41 publications

References 12 publications

Active site directed irreversible inhibition of thermolysin

Active site directed irreversible inhibition of thermolysin

Synthesis of 125I labeled N-3-(4-hydroxyphenyl)propionyl aminoacyl transfer ribonucleic acids

Radiometric techniques for the detection and assessment of tritium in aqueous media - a review

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Synthesis of ¹²⁵I labeled N-3-(4-hydroxyphenyl)propionyl aminoacyl transfer ribonucleic acids