Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion

Assis, Lívia; Moretti, Ana Iochabel Soares; Abrahão, Thalita B.; Souza, Heraldo Possolo de; Hamblin, Michael R.; Parizotto, Nivaldo Antônio

doi:10.1007/s10103-012-1183-3

Cited by 100 publications

(98 citation statements)

References 40 publications

(53 reference statements)

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“…In a time‐dependent manner, red and NIR therapies are also able to modulate the gene expression of mediators, such as TNF‐ α , 15, 16 IL1‐ β , 17 IL‐6 18 and TGF‐ β16, 19 as well as genes involved in the differentiation of myogenic stem cells, such as MyoD 18, 20 and myogenin 18, 20 during the muscle repair process.…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation and different macrophages phenotypes during muscle tissue repair

Souza

Mesquita‐Ferrari

Rodrigues

et al. 2018

J Cellular Molecular Medi

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Macrophages play a very important role in the conduction of several regenerative processes mainly due to their plasticity and multiple functions. In the muscle repair process, while M1 macrophages regulate the inflammatory and proliferative phases, M2 (anti‐inflammatory) macrophages direct the differentiation and remodelling phases, leading to tissue regeneration. The aim of this study was to evaluate the effect of red and near infrared (NIR) photobiomodulation (PBM) on macrophage phenotypes and correlate these findings with the repair process following acute muscle injury. Wistar rats were divided into 4 groups: control; muscle injury; muscle injury + red PBM; and muscle injury + NIR PBM. After 2, 4 and 7 days, the tibialis anterior muscle was processed for analysis. Macrophages phenotypic profile was evaluated by immunohistochemistry and correlated with the different stages of the skeletal muscle repair by the qualitative and quantitative morphological analysis as well as by the evaluation of IL‐6,TNF‐α and TGF‐β mRNA expression. Photobiomodulation at both wavelengths was able to decrease the number of CD68+ (M1) macrophages 2 days after muscle injury and increase the number of CD163+ (M2) macrophages 7 days after injury. However, only NIR treatment was able to increase the number of CD206+ M2 macrophages (Day 2) and TGF ‐β mRNA expression (Day 2, 4 and 7), favouring the repair process more expressivelly. Treatment with PBM was able to modulate the inflammation phase, optimize the transition from the inflammatory to the regeneration phase (mainly with NIR light) and improve the final step of regeneration, enhancing tissue repair.

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Section: Introductionmentioning

confidence: 99%

Photobiomodulation and different macrophages phenotypes during muscle tissue repair

Souza

Mesquita‐Ferrari

Rodrigues

et al. 2018

J Cellular Molecular Medi

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“…Considerando os critérios de inclusão adotados, foram encontrados um total de 30 estudos nas bases de dados consultadas, dos quais quinze (50%) foram realizados em tendão 3,4,6,9,[22][23][24][31][32][33][34][35][36][37][38] , dez (33,33%) em musculos 12,14,[39][40][41][42][43][44][45] , e cinco (16,66%) em osso e/ou articulação 13,25,[46][47][48] Os resultados mais expressivos obtidos com a terapia foram: diminuição da resposta inflamatória mediante redução de células inflamatórias e modulação de mediadores da inflamação (36,66%), e o aumento da síntese de colágeno (13,33%). Alguns dos demais resultados (50,01%) mencionados foram: angiogênese; redução do estresse oxidativo, do colágeno e da fibrose; aumento do tecido de granulação, da remodelação e síntese óssea, assim como da expressão da proteína da diferenciação miogênica (MyoD) e do fator de crescimento endotelial vascular (VEGF).…”

Section: Resultsunclassified

“…Para as afecções musculoesqueléticas são disponibilizados os lasers à base de arseneto de gálio (Ga-As) e hélio-neônio (He-Ne) 6,11 . Também existem estudos utilizando o laser à base de arseneto de gálio associado a alumínio (GaAlAs) [12][13][14] .…”

Section: Introductionunclassified

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Laserterapia Em Afecções Locomotoras: Revisão Sistemática De Estudos Experimentais

Souza

Silva

2016

Rev Bras Med Esporte

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RESUMOIntrodução: Ao longo dos anos a fisioterapia tem contribuído para o tratamento das lesões musculotendí-neas e osteoarticulares pela utilização de diversos recursos eletrotermofototerapêuticos como, por exemplo, o laser de baixa potência. Objetivo: A finalidade desse estudo foi identificar mediante revisão sistemática, o efeito da laserterapia de baixa potência em afecções do sistema locomotor de ratos, com destaque para a dose de energia e o comprimento de onda utilizado, assim como para a resposta ao tratamento. Métodos: Foram consultadas as bases de dados Medline, Lilacs, PEDro e SciELO, entre janeiro de 2005 a maio de 2013, utilizando os termos "tendinopathy", "laser therapy", "rats", "tendon", "muscle", "bone", "low-level laser therapy", assim como "tendinopatia", "laserterapia", "ratos", "tendão", "músculo", "osso" e "laser de baixa potência". Apenas foram incluídos estudos experimentais publicados nos idiomas português e inglês, que induziram lesão em tendões, músculos, ossos e/ou articulação, tratada com laserterapia, associada ou não a outros tratamentos. Resultados: Foi encontrado um total de 30 estudos experimentais nas bases de dados consultadas, dos quais 15 (50%) foram realizados em tendão, 10 (33,33%) em músculo e cinco (16,66%) em osso e/ou articulação. As doses de energia mais comumente utilizadas foram de 3 J (26,66%) e 1 J (16,66%). Já os comprimentos de onda foram de 904 nm (21,21%) e 830 nm (21,21%). Conclusão: A informação mais expressiva obtida com a terapia foi a diminuição da resposta inflamatória (36,66%) em lesões ortopédicas agudas. May 2013, using the terms "tendinopathy", "laser therapy", "rats", "tendon", "muscle", "bone", "low-level laser therapy", as well as "tendinopatia", "laserterapia", "ratos", "tendão", " músculo", "osso" and "laser de baixa potência". Only experimental studies published in Portuguese and English and that induced lesion in tendons, muscles, bones and/or joints, treated Descritores

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“…Moreover, several experimental studies from our group have observed the potential effects of both LLLT on muscle damage [20][21][22] and LLLT associated with physical training on muscle performance 23,24 in Wistar rats. However, to our knowledge, the associated effects of LLLT and a physical exercise program on muscle repair have not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

Adabbo

Paolillo

Bossini

et al. 2016

Photomedicine and Laser Surgery

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Objective: The aim of this study was to analyze the effects of low-level laser therapy (LLLT) when associated with treadmill training on the recovery of skeletal muscle, during two periods of rest after muscle injury in rats. Background data: Because of photostimulation, LLLT has been presented as an alternative for accelerating the tissue healing process. Materials and methods: Forty rats were divided into two groups (A and B) containing four subgroups each: GC (Control Group)-cryolesion untreated; EG (Exercise Group)-cryolesion treated with physical exercise; LG (Laser Group)-cryolesion treated with laser; ELG (Exercise and Laser Group)-cryolesion treated with laser and physical exercise. The right tibialis anterior (TA) of the middle belly was injured by a cooling iron bar (cryoinjury). Group A remained at rest for 3 days, whereas Group B remained at rest for 7 days. The laser parameters utilized were 780 nm with 15 mW average optical power and spot size of 0.04 cm 2 applied during 10 sec, leading to 0.152 J and 3.8 J/cm 2 . Treadmill training with and without laser application was performed during 5 days, with each session lasting for 12 min at a velocity of 17 m/min. Subsequently, the TA muscle was removed for a histological and morphometric analysis. Results: The damaged area was significantly smaller for the ELG at both periods of rest, 3 and 7 days, respectively (4.4 -0.42% and 3.5 -0.14%, p < 0.05), when compared with the LG (18.6 -0.64% and 7.5 -0.13%), the EG (21 -0.26% and 8.7 -0.32%), and the CG (23.9 -0.37% and 21.4 -0.38%). In addition, the number of blood vessels were significantly higher for the ELG at both periods of rest, 3 and 7 days, respectively (71.2 -13.51 and 104.5 -11.78, p < 0.05), when compared with the LG (60.6 -11.25 and 93.5 -16.87), the EG (51.6 -7.3 and 93.8 -15.1) and the CG (34.4 -2.54 and 65.7 -14.1). Conclusions: The LLLT applied before the physical exercise on the treadmill stimulated the angiogenesis and accelerated the process of muscle recovery.

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Low-level laser therapy (808 nm) contributes to muscle regeneration and prevents fibrosis in rat tibialis anterior muscle after cryolesion

Cited by 100 publications

References 40 publications

Photobiomodulation and different macrophages phenotypes during muscle tissue repair

Photobiomodulation and different macrophages phenotypes during muscle tissue repair

Laserterapia Em Afecções Locomotoras: Revisão Sistemática De Estudos Experimentais

Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

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