Low-Level Laser Therapy and Calcitonin in Bone Repair: Densitometric Analysis

Abstract

The aim of this work was to evaluate the association of low-level laser therapy (LLLT, 830 nm) and calcitonin in bone repair considering that bone healing remains a challenge to health professionals. Calcitonin has antiosteoclastic action and LLLT is a treatment that uses low-level lasers or light-emitting diodes to alter cellular function. Both are used to improve bone healing. Densitometry is a clinical noninvasive valuable tool used to evaluate bone mineral density (BMD). Sixty male rats were submitted to bone defect with a trephine bur, randomly divided into four groups of 15 animals each: control (C); synthetic salmon calcitonin (Ca); LLLT (La); LLLT combined with calcitonin (LaCa). Animals from Ca and LaCa received 2 UI/Kg synthetic salmon calcitonin intramuscularly on alternate days after surgery. Animals from groups La and LaCa were treated with infrared LLLT (830 nm, 10 mW, 20 J/cm2, 6 s, contact mode). Five animals from each group were euthanized 7, 14, and 21 days after surgery and bone defects were analyzed by densitometry. Statistical analysis showed a significant difference in BMD values in LaCa group at 7 and 21 days (). The results of the densitometric study showed that LLLT (830 nm) combined with calcitonin improved bone repair.

Effects of photobiomodulation on bone defects grafted with bone substitutes: A systematic review of in vivo animal studies

“…Eight studies were excluded as they were based on utilization of autogenous bone grafts for bone repair and regeneration [24, 27, 36–41] (inter‐reviewer agreement, κ = 0.94). Thus, out of the fifty‐three full text articles, fifteen articles were excluded due to the above mentioned reasons and 38 articles were included and analyzed in the present systematic review [42–79]. All the included articles were in vivo animal studies.…”

“…Thirty‐one of the thirty‐eight studies included in this review were conducted in Brazil [42–72]. Two studies have been conducted in Egypt [73, 74].…”

“…In thrity‐three papers, the experiments were conducted on Wistar rats (male and/or female) [42–73, 79]. The Sprague–Dawley rats were used, as experimental models in two studies [75, 76], whereas, the New Zealand rabbits were the experimental models in three studies [74, 77, 78] (Table 1).…”

“…This study concluded that Biosilicate presented an osteogenic activity and bone repair acceleration, however PBMT was unable to enhance the bioactive properties of the Biosilicate. Interestingly, a study by Ribeiro et al [56] demonstrated that PBM as a mono-therapy or in association with bone substitute (calcitonin), showed a significant improvement in bone repair and regeneration. Two studies of this systematic [64,65] showed an increase in bone remodeling process at a late stage of healing when PBMT utilized with bone grafted substitutes to intensify its effects, by improving the mineralization process and increasing the deposition of calcium hydroxyapatite [65].…”

“…Biosilicate was utilized in five articles [47,49,53,57,64]. In three studies, mineral tri-oxide aggregate (MTA) was used [55][56][57]. The BMPs were utilized in six articles [46, 50-52, 54, 55].…”