Low-level laser therapy with helium–neon laser improved viability of osteoporotic bone marrow-derived mesenchymal stem cells from ovariectomy-induced osteoporotic rats

Fallahnezhad, Somaye; Piryaei, Abbas; Tabeie, Faraj; Nazarian, Hamid; Darbandi, Hasan; Amini, Abdollah; Mostafavinia, Ataroalsadat; Ghorishi, Seyed Kamran; Jalalifirouzkouhi, Ali; Bayat, Mohammad

doi:10.1117/1.jbo.21.9.098002

Cited by 21 publications

(24 citation statements)

References 47 publications

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“…The osteogenic induction medium culture medium was replaced by fresh medium immediately before irradiation. The LLLT protocol (He‐Ne laser, 632.8 nm, 3 mW, 1.2 J/cm 2 , 378 s, 1.56 cm laser beam diameter, 1.96 cm 2 laser beam surface area, and 0.0016 W/cm 2 ) was the same as in our previous study . The He‐Ne laser beam was expanded and collimated.…”

Section: Methodsmentioning

confidence: 99%

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Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

Fallahnezhad

Piryaei

Darbandi

et al. 2017

J of Cellular Biochemistry

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Postmenopausal osteoporosis (OP) is a major concern for public health. Low-level laser therapy (LLLT) has a positive effect on the health of bone marrow mesenchymal stem cells (BMMSCs). The purpose of this study is to evaluate the influence of LLLT and oxytocin (OT) incubation-individually and in combination-on osteoporotic BMMSCs in ovariectomized rats. Twelve female rats were randomized into two groups to undergo either a sham surgery (sham group) or ovariectomy-induced osteoporosis (OVX group). MSCs harvested from the BM of healthy and OVX rats underwent culture expansion. There were five groups. In Groups one (sham-BMMSC) and two (OVX-BMMSC) the cells were held in osteogenic condition medium without any intervention. In the group three (OT), OT incubation with optimum dose was performed for 48 h (two times, 10 −12 molar). In Group four, lasertreated-OVX-BMMSCs were treated with optimum protocol of LLLT (one time, 1.2 J/cm 2 ). In Group five (laser + OT group), the OT incubation plus the laser irradiation was performed. The biostimulatory effect of LLLT is demonstrated by a significant increase in the viability of OVX-BMMSCs, cell cycle, and extracellular levels of Transforming growth factor beta (TGF-β), insulin-like growth factor-I (IGF-I), and Alkaline phosphatase (ALP) compared to control OVX-BMMSCs and/or the sham group. OT incubation and laser + OT incubation have a positive effect on OVXBMMSCs. However, LLLT is more effective statistically. We conclude that LLLT significantly improved cell viability, enhanced the osteogenic potential of the OVXBMMSCs, and increased the extracellular levels of the TGF-β, IGF-I, and ALP.

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Section: Methodsmentioning

confidence: 99%

“…The absorbance of the formazan solution from the wells was calculated on an ELISA reader at a wavelength of 570 nm using microtiter plate reader (Anthos 2020 Microplate Readers, Biochrom Ltd., Cambridge, UK) and optical densities (OD) were reported. A 620 nm filter was used for background reading …”

Section: Methodsmentioning

confidence: 99%

Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

Fallahnezhad

Piryaei

Darbandi

et al. 2017

J of Cellular Biochemistry

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“…It was shown that a low-level laser in the osteoporosis MSCs from ovariectomized mice could significantly increase the optical density and cell viability compared to the control group. 39,40 Also, another study showed that the effects of a low-power laser strongly depended on wavelength parameters, the number of laser therapy sessions, the state of MSC physiology, and the type of the laser. 40 In Europe, about 1.5 million patients undergo jaw repair every year.…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 99%

“…39,40 Also, another study showed that the effects of a low-power laser strongly depended on wavelength parameters, the number of laser therapy sessions, the state of MSC physiology, and the type of the laser. 40 In Europe, about 1.5 million patients undergo jaw repair every year. Despite surgical interventions, about 20% of them still have dysfunction.…”

Section: Bone Marrow Mesenchymal Stem Cellsmentioning

confidence: 99%

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

et al. 2019

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Introduction: The purpose of this study is to investigate the effect of a low-power laser on the proliferation, migration, differentiation of different types of mesenchymal stem cells (MSCs) in different studies. Methods: The relevant articles that were published from 2004 to 2019 were collected from the sources of PubMed, Scopus, and only the articles specifically examining the effect of a lowpower laser on the proliferation, differentiation, and migration of the MSCs were investigated. Results: After reviewing the literature, only 42 articles were found relevant. Generally, most of the studies demonstrated that different laser parameters increased the proliferation, migration, and differentiation of the MSCs, except the results of two studies which were contradictory. In fact, changing the parameters of a low-power laser would affect the results. On the other hand, the source of the stem cells was reported as a key factor. In addition, the combination of lasers with other therapeutic approaches was found to be more effective. Conclusion: The different parameters of lasers has been found to be effective in the proliferation, differentiation, and migration of the MSCs and in general, a low-power laser has a positive effect on the MSCs, helping to improve different disease models.

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“…5 Wound healing is a complicated process that involves clot formation, inflammation, granulation, epithelialization, and remodeling. 7,8 Studies on wound healing have investigated the effect of oxygen therapy, [9][10][11] biomechanics, 12 radiotherapy and medical pomades. 13 Low-level laser therapy (LLLT) is a good modality for wound healing.…”

Section: Introductionmentioning

confidence: 99%

Combined Effect of Low-Level Laser Treatment and Levothyroxine on Wound Healing in Rats With Hypothyroidism

et al. 2018

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Please cite this article as follows: Firouzi A, Norozian M, Amini A, Abdollahifar MA, Abbaszadeh HA, Fadaei Fathabadi F. Combined effect of low-level laser treatment and levothyroxine on wound healing in rats with hypothyroidism. AbstractIntroduction: Hypothyroidism delays wound healing by reducing the synthesis of keratinocytes, fibroblast cells, and collagen. Methods for enhancement of wound healing include laser therapy and hormone therapy. The current study evaluated the combined effect of laser and levothyroxine therapy to cure wounds in male rats with hypothyroidism. Methods: Sixty male Wistar rats were randomly divided into 5 groups: (1) healthy controls;(2) controls with hypothyroidism; (3) hypothyroidism + laser treatment; (4) hypothyroidism + levothyroxine treatment; (5) hypothyroidism + laser + levothyroxine treatment. Hypothyroidism was induced by dissolving 4 mg of methimazole in 100 mL of drinking water daily for 28 days. After hypothyroidism had been confirmed, a longitudinal incisional wound was created on the dorsal rib cages of the rats. The wounds that received laser treatment were divided into 12 sections and treated at 810 nm wavelength and 0.2 J/cm 2 of energy density for 200 seconds. Levothyroxine was administrated in doses of 20 µg/kg/d i.p. All groups were divided into 3 subgroups for testing on days 4, 7 and 14. Samples were collected in all the subgroups. Results: The results showed that hypothyroidism reduced fibrous tissue volume, fibroblasts, and basal cell numbers. The combined effect of laser and levothyroxine improved all parameters. Conclusion: Combined laser and levothyroxine treatment showed the best effect on wound healing and accelerated the closure of the wounds.

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Low-level laser therapy with helium–neon laser improved viability of osteoporotic bone marrow-derived mesenchymal stem cells from ovariectomy-induced osteoporotic rats

Cited by 21 publications

References 47 publications

Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

Effect of low‐level laser therapy and oxytocin on osteoporotic bone marrow‐derived mesenchymal stem cells

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

Combined Effect of Low-Level Laser Treatment and Levothyroxine on Wound Healing in Rats With Hypothyroidism

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