Low Levels of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, and Wound Healing

Pan, Qing; Qiu, Weiqiang; Huo, Ya Nan; Yao, Yu; Lou, Marjorie F.

doi:10.1167/iovs.10-5866

Cited by 76 publications

(59 citation statements)

References 37 publications

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“…S3A and B in the supplemental material), two inhibitors of H 2 O 2 -induced EGFR activation in cancer cells (44,47). These results indicate that E. faecalis-induced cell proliferation depends on H 2 O 2 production and extend the recent finding that H 2 O 2 promotes cell proliferation through EGFR activation (54). Our data suggest that activation of EGFR by E. faecalis may result in an uncontrolled proliferative signal to various cell types inside the tumor microenvironment, contributing to tumor progression.…”

Section: Discussionsupporting

confidence: 80%

“…5A and D). These results are supported by several studies showing that H 2 O 2 alone can enhance EGFR activation (38,42,54,59). The precise mechanism by which H 2 O 2 activates EGFR is not known, but H 2 O 2 may promote pEGFR activity by releasing EGFR dimerization constraints under physiologic conditions.…”

Section: Discussionsupporting

confidence: 70%

“…Our findings are in agreement with previous studies that support a role for H 2 O 2 in the EGFR signaling pathway (18,58) and demonstrate that H 2 O 2 treatment initiates stimulation of the EGFR and ectodomain shedding of the EGFR and EGF-like ligands via MMP activation. The EGFR activation and upregulation of these ligands then elicit signaling events through EGFR/MEK/ERK phosphorylation, thereby contributing to cell proliferation (18,42,47,54,57,58).…”

Section: Discussionmentioning

confidence: 99%

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Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation

et al. 2012

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f Enterococcus faecalis is a member of the intestinal and oral microbiota that may affect the etiology of colorectal and oral cancers. The mechanisms by which E. faecalis may contribute to the initiation and progression of these cancers remain uncertain. Epidermal growth factor receptor (EGFR) signaling is postulated to play a crucial role in oral carcinogenesis. A link between E. faecalis and EGFR signaling in oral cancer has not been elucidated. The present study aimed to evaluate the association between E. faecalis and oral cancer and to determine the underlying mechanisms that link E. faecalis to EGFR signaling. We report the high frequency of E. faecalis infection in oral tumors and the clinical association with EGFR activation. Using human oral cancer cells, we support the clinical findings and demonstrate that E. faecalis can induce EGFR activation and cell proliferation. E. faecalis activates EGFR through production of H 2 O 2 , a signaling molecule that activates several signaling pathways. Inhibitors of H 2 O 2 (catalase) and EGFR (gefitinib) significantly blocked E. faecalis-induced EGFR activation and cell proliferation. Therefore, E. faecalis infection of oral tumor tissues suggests a possible association between E. faecalis infection and oral carcinogenesis. Interaction of E. faecalis with host cells and production of H 2 O 2 increase EGFR activation, thereby contributing to cell proliferation.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

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Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation

et al. 2012

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“…2A,A′). Although we cannot exclude direct ROSinduced chemotaxis (Hurd et al, 2012;Pan et al, 2011), ROSmodulated electrotaxis might be the major cue (Zhao, 2009). This rationale could apply to axonal sprouting and to axonal growth (electrotropism).…”

Section: Homeostatic (From 48 Hpa)mentioning

confidence: 91%

Early bioelectric activities mediate redox-modulated regeneration

et al. 2016

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Reactive oxygen species (ROS) and electric currents modulate regeneration; however, the interplay between biochemical and biophysical signals during regeneration remains poorly understood. We investigate the interactions between redox and bioelectric activities during tail regeneration in Xenopus laevis tadpoles. We show that inhibition of NADPH oxidase-mediated production of ROS, or scavenging or blocking their diffusion into cells, impairs regeneration and consistently regulates the dynamics of membrane potential, transepithelial potential (TEP) and electric current densities (J I ) during regeneration. Depletion of ROS mimics the altered TEP and J I observed in the non-regenerative refractory period. Short-term application of hydrogen peroxide (H 2 O 2 ) rescues (from depleted ROS) and induces (from the refractory period) regeneration, TEP increase and J I reversal. H 2 O 2 is therefore necessary for and sufficient to induce regeneration and to regulate TEP and J I . Epistasis assays show that voltage-gated Na + channels act downstream of H 2 O 2 to modulate regeneration. Altogether, these results suggest a novel mechanism for regeneration via redoxbioelectric orchestration.

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“…6 and Table 1). Recent in vitro and in vivo observations suggest that ROS, and mainly H 2 O 2 , interfere with cell signaling acting like a second messenger and inducing adaptive responses, and this is particularly observed in skin wound healing where cells are exposed to H 2 O 2 following injury (Atrux-Tallau et al, 2011;Pan et al, 2011;Schreml et al, 2011;Eligini et al, 2009). Therefore, it is suggested that the disinfection technique does not have injurious effect on the healing process of the wounded skin, and rather have a healing effect likely attributable to H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats

et al. 2012

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Low Levels of Hydrogen Peroxide Stimulate Corneal Epithelial Cell Adhesion, Migration, and Wound Healing

Abstract: These findings suggest that H(2)O(2) at low levels promotes cell adhesion, migration, and wound healing in cornea cells or tissue, and the interaction of H(2)O(2) with Src plays a major role.

Cited by 76 publications

References 37 publications

Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation

Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation

Early bioelectric activities mediate redox-modulated regeneration

Topical treatment of oral cavity and wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats

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