Low-molecular-weight components of the metabolome control the proliferative activity in cellular and bacterial cultures

Abstract

The effects of blood metabolites and model cell culture exometabolites found in the human and animal blood metabolomes have been assessed. Specifically, the influence of carboxylic acids that are structural analogues of amino acids and the drug Aktoflor-C have been studied. Methods of organotypic culturing of rat tissues and Escherichia coli bacterial culturing have been used. It has been found that all the tested compounds either stimulate or inhibit cell proliferation in tissue cultures and proliferation in bacterial cultures. The metabolites that are present in blood and interstitial fluids can exert regulation effects on the tissues of the body and intestinal microbiota.

Identification of Antihypertensive Tripeptides in the Culture Medium of Lactobacillus helveticus D75 and D76 Strains

“…In conclusion, it can be noted that the fact that biologically active compounds produced by probiotic strains of L. helveticus D75 and D76 are detected in the culture fluid, taking into account the genetic potential of these microorganisms, allows us be optimistic that there will be experimental and clinical confirmation in subsequent studies of other beneficial effects for human health associated with their metabolic activity and the production of exometabolites [16]. In addition to the known effects, such as antagonistic activity against pathogenic microorganisms, including Candida, immunomodulatory action, and production of antihypertensive peptides [3,6,15], probiotic bacteria L. helveticus, according to the results of recent scientific studies, can reduce the levels of cholesterol (cholesterol-lowering effect) and low-density lipoproteins in the blood (hypolipidemic effect), improve digestion (α-and β-galactosidase activity), strengthen the mucosal barrier and suppress inflammatory processes in the intestine (anti-inflammatory effect), inhibit colorectal oncogenesis (antitumor effect), modulate microbial homeostasis (production of bacteriocins), provide antioxidant and anti-aging effects, and improve liver function when combined with appropriate diet and exercise [6,17].…”

The role of intestinal disbiosis in the development of mitochondrial dysfunction and nonalcoholic fatty liver disease

“…Метабиотики продемонстрировали потенциальную эффективность в экспериментальных и клинических исследованиях. Так, например, Актофлор-С оказывает положительное действие не только на микробиоту, но и на организм человека, стимулируя регенерацию кишечного эпителия и работу иммунной системы [53]. Принимая во внимание вероятную патогенетическую роль микробных эндотоксинов (ЛПС) и бактериального этанола при НАЖБП, клинически перспективными представляются возможности метабиотиков по снижению негативных эффектов этанола, восстановлению нарушенной функции белков плотных контактов, улучшению кишечного барьера, уменьшению алкоголь-индуцированного повреждения печени и потенциальному антиканцерогенному действию (профилактика гепатоцеллюлярной карциномы) [55].…”