Low molecular weight lipoproteins in the haemolymph of the silkworm, Bombyx mori: Inheritance, isolation and some properties

Gamo, Takuma

doi:10.1016/0020-1790(78)90059-8

Cited by 30 publications

(28 citation statements)

References 18 publications

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“…Fat body cells of the final (fifth) instar larvae synthesize massive quantities of plasma proteins, releasing them into the hemolymph (Gamo 1978;Tojo et al 1980;Tomino 1985). In B. mori, storage proteins occur in two forms, which are termed "SP1" and "SP2," respectively.…”

Section: Protein Synthesis Of Fat Body Cells In Primary Culturementioning

confidence: 99%

“…In the silkworm, Bombyx mori, two groups of proteins referred to as "storage proteins" (SP) and "30K proteins," respectively, comprise major protein constituents of the larval hemolymph (Gamo 1978;Tojo et al 1980;Tomino 1985). These proteins are synthesized by the fat body cells and released into the hemolymph in a time-dependent sequential fashion during post-embryonic development and metamorphosis (Gamo 1978;Tojo et al 1980;Tomino 1985). The genomic as well as mRNA sequences for these proteins have been cloned, and their structures have been elucidated Fujii et al 1989;Mori et al 1991).…”

Section: Introductionmentioning

confidence: 99%

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Biosynthesis of major plasma proteins in the primary culture of fat body cells from the silkworm, Bombyx mori

Kishimoto¹,

Nakato²,

Izumi³

et al. 1999

Cell and Tissue Research

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Plasma proteins termed "SP1" and "30K proteins" are synthesized by the fat body cells of the silkworm, Bombyx mori, in a sex- and stage-specific manner during larval development. We successfully established a primary culture of the fat body cells in order to investigate the regulatory mechanisms of plasma protein gene expression. The primary cultures of fat body cells contained at least two cell types: small oval cells, and large spherical cells. The cells adhered to and migrated on the cultured dish after plating. By the 7th day of cultivation, the cells clustered to form fat body-like structures, which were maintained for at least 3 months. Plasma proteins were actively synthesized in the primary cultures of the fat body cells isolated from the final instar larvae only when the cells tightly adhered to and clustered on the cultured dish. Immunocytochemical analysis revealed that only 10-15% of the clustered cells synthesized plasma proteins in our culture system, indicating that the primary culture comprises heterogeneous cells that are morphologically and functionally distinct. The patterns of SP1 syntheses in primary cultures faithfully reproduced their sex-dependency in vivo.

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Section: Protein Synthesis Of Fat Body Cells In Primary Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biosynthesis of major plasma proteins in the primary culture of fat body cells from the silkworm, Bombyx mori

Kishimoto¹,

Nakato²,

Izumi³

et al. 1999

Cell and Tissue Research

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“…They have further indicated that the biosynthesis of 30K proteins in the fat body is regulated during the development at the pretranslational level. Gamo [23] has studied three kinds of lipoproteins with low molecular weights in the silkworm hemolymph. The developmental profiles of these lipoproteins in the hemolymph are very similar to those of MHPs, showing stage specificity from the late larval to the middle period of the pharate adult.…”

Section: Discussionmentioning

confidence: 99%

Effect of metamorphosis on the major hemolymph proteins of the silkworm

Seong

Park

Nagata

et al. 1985

Arch Insect Biochem Physiol

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During the metamorphosis of the silkworm, Bombyx mori, three major hemolymph proteins (MHPs) (molecular weights 17,000, 25,000, 27,000) were detected and found to be distributed in the hernolymph and in the tissues of several organs, such as the fat body, midgut, ovary, testis, and even eggs. The M H P s in eggs gradually decreased and disappeared during embryogenesis. The formation, distribution, and utilization of M H P s in tissues other than the gonad, however, were not affected by sex.Radioisotope experiments in vivo revealed that the MHPs were synthesized at an early period of the fifth larval instar. The synthesis of at least two of them occurred in the fat body. M H P s in the hemolymph entered the tissues at the onset of the larval-pupal transformation. On the basis of their appearance, distribution, and depletion, the M H P s may be classified as reserve proteins which are synthesized in the larval stage and utilized later in the developmental stages.

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“…Electrophoretic variants are widespread in the silkworm, Bombyx mori (Yoshitake and Akiyama, 1964;Eguchi et al, 1965Eguchi et al, , 1984Eguchi and Yoshitake, 1967;Gamo, 1968Gamo, , 1978Gamo, , 1982 as well as other animals. It is noteworthy, however, that the phosphatase E is an isozyme showing different properties, that is, acid phosphatase isozymes would comprize heterogeneity not only in non-catalytic sites but also in the catalytic site.…”

Section: Discussionmentioning

confidence: 99%

A novel variant of acid phosphatase isozyme from hemolymph of the silkworm, Bombyx mori.

Eguchi

Takahama

Ikeda

et al. 1988

The Japanese journal of genetics

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Four electrophoretic variants of acid phosphatase have been already reported in the silkworm hemolymph. We found an electrophoretic variant (type E) which had been designated 0 (null) type since the distinct band had not been detected.The band E was undiscernible when the substrate (a-naphthyl phosphate) and chromogen (Fast blue B) were poured simultaneously on the gel after electrophoresis according to the former procedure.However, if the gel was incubated with a-NP then Fast blue B was added, a distinct band E appeared. Quantitative analyses showed that Fast blue B inhibited the isozyme E competitively but other isozymes noncompetitively.Several lines of experimental evidence suggest the isozyme E is not only a charge isomer but also an isozyme showing different kinetic properties with other isozymes. By crossing experiments isozymes A-E were considered to be controlled by codominant alleles, BphA-BphE, respectively.

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Low molecular weight lipoproteins in the haemolymph of the silkworm, Bombyx mori: Inheritance, isolation and some properties

Cited by 30 publications

References 18 publications

Biosynthesis of major plasma proteins in the primary culture of fat body cells from the silkworm, Bombyx mori

Biosynthesis of major plasma proteins in the primary culture of fat body cells from the silkworm, Bombyx mori

Effect of metamorphosis on the major hemolymph proteins of the silkworm

A novel variant of acid phosphatase isozyme from hemolymph of the silkworm, Bombyx mori.

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