Low molecular weight organic acids released from roots of durum wheat and flax into sterile nutrient solutions

Cieśliński, G.; Rees, K. C. J. Van; Szmigielska, Anna M.; Huang, P. M.

doi:10.1080/01904169709365291

Cited by 60 publications

(23 citation statements)

References 21 publications

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“…Both of the nutrient solutions used to stimulate competence development in Acinetobacter sp. strain BD413 populations contained inorganic salts and, in addition, simple organic compounds corresponding to those which have been frequently detected in the rhizosphere of various plants (4,6,12,27). As seen from Table 1, solution B was efficient at inducing Acinetobacter sp.…”

mentioning

confidence: 68%

Transformation of Acinetobacter sp. Strain BD413(pFG4Δ nptII ) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Nielsen

Elsas

Smalla

2000

Appl Environ Microbiol

107

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Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 10 9 CFU/g of soil to below detection. In contrast to a chromosomal nptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.

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mentioning

confidence: 68%

Transformation of Acinetobacter sp. Strain BD413(pFG4Δ nptII ) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Nielsen

Elsas

Smalla

2000

Appl Environ Microbiol

107

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“…In another hydroponics study using maize (Zea mays L.), succinic (40%), citric (30%), lactic (15%), and malic (15%) acids at the relative amounts indicated in parenthesis were detected 40) . Cieśliński et al 12) examined the composition of organic acids from 5 different durum wheat cultivars in standard nutrient solution and reported that oxalic, fumaric, succinic, acetic and tartaric acids were found in root exudates with oxalic and acetic acids being the predominant acids, but when they determined the organic acids exuded from the same cultivar in Cd contaminated soils the predominant organic acids was different from those previously detected in the hydroponic study. For the soil experiments acetic and succinic acids accounted for 93.2, 88.4 and 84.2% of the total amount of LMWOAs present for the three soils studied 9) .…”

Section: Nature Of Organic Acids In the Root Exudatesmentioning

confidence: 99%

“…Numerous researches were conducted to understand the role of root exudates in metal translocation and to identify the specific compounds comprising root exudates 4,[9][10][11] . From these studies low molecular weight (LMW) compounds such as amino acids, organic acids, sugars, phenolics, and various other secondary metabolites are believed to comprise the majority of root exudates, with high molecular weight (HMW) compounds including mucilage and proteins being of secondary importance 9,11,12) . The exact constitution of any specific root exudate is variable being highly dependent upon plant species and soil type.…”

Section: Introductionmentioning

confidence: 99%

Lead Induced Organic Acid Exudation and Citrate Enhanced Pb Uptake in Hydroponic System

Kim¹,

Owens²,

Naidu³

et al. 2009

Korean Journal of Environmental Agriculture

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The influence of Pb-citrate complex formation on Pb uptake and the effect of Pb on organic acid exudation were investigated using four plant species, viz., sunflower (Helianthus annuus L), Indian mustard (Brassica juncea), canola (Brassica napus) and vetiver grass (Vetiveria zizanioides) under hydroponic conditions. Seedlings were exposed to different levels of Pb and Pb-citrate for 24 hrs and subsequently Pb distributions in plant shoot, root and hydroponic solution were measured. . This result showed enhanced translocation of Pb from root to shoot with observation of transfer coefficient (Kt) increase from 2.03E-3 to 5.72E-3.

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“…Plant roots are known to excrete a range of organic compounds into the media immediately surrounding the roots (Cieslinski et al, 1997). These compounds modify solution pH and provide a source of ligands that have the potential to complex the metal being studied.…”

Section: Rooted Plantsmentioning

confidence: 99%

Coupling the use of computer chemical speciation models and culture techniques in laboratory investigations of trace metal toxicity

Twiss

Errécalde²,

Fortin

et al. 2001

Chemical Speciation & Bioavailability

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Chemical Speciation and Bioavailability (2001), 13(1) ABSTRACTThe bioavailability and toxicity of a dissolved metal are closely linked to the metal's chemical speciation in solution. A variety of inorganic and organic ligands are often used in laboratory toxicity tests to control the concentration of labile trace metal in solution. Computerised chemical speciation models based on thermodynamic principles can be used to estimate metal speciation under such experimental conditions. However, these models are sensitive to the quality of their thermodynamic databases. Detailed protocols for the incorporation of reliable equilibrium formation constants into widely available computer chemical speciation programs (e.g., MINEQL+ and MINTEQ) are provided. The examples demonstrate both the benefits and the potential pitfalls involved in the use of chemical speciation models. The application of chemical speciation modelling to metal toxicity studies is discussed and guidelines are proposed for its proper use. Both defined media and chemical speciation programs have co-existed for two decades but the combined use of these techniques has been reserved for those possessing in-depth knowledge of both chemistry and biology. The techniques presented should enable an investigator with basic biological, chemical and computing skills to design an aqueous medium and incorporate correct thermodynamic constants into a computer chemical speciation program, starting from a standardised database, thereby providing a sound framework for critically assessing the biological response of a particular test organism to a given metal.

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Low molecular weight organic acids released from roots of durum wheat and flax into sterile nutrient solutions

Cited by 60 publications

References 21 publications

Transformation of Acinetobacter sp. Strain BD413(pFG4Δ nptII ) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Transformation of Acinetobacter sp. Strain BD413(pFG4Δ nptII ) with Transgenic Plant DNA in Soil Microcosms and Effects of Kanamycin on Selection of Transformants

Lead Induced Organic Acid Exudation and Citrate Enhanced Pb Uptake in Hydroponic System

Coupling the use of computer chemical speciation models and culture techniques in laboratory investigations of trace metal toxicity

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