Low-molecular weight organic compounds (LMWOC) of water extracts from Cistanche deserticola stolones depending on treatment

Sarsenbayev, Kanat; Sarsenbayeva, Aliya B.; Balmukanov, Kuandyk U.; Кожамжарова, Л С

doi:10.1016/j.jbiotec.2014.07.369

Cited by 2 publications

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“…The major bioactive components of C . deserticola are Phenylethanoid glycosides (PheGs, PhGs) [ 2 , 9 , 10 , 14 , 15 ]. To date, more than 20 phenylethanoid glycosides have been isolated from the succulent stem of C .…”

Section: Introductionmentioning

confidence: 99%

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RNA-Seq Based De Novo Transcriptome Assembly and Gene Discovery of Cistanche deserticola Fleshy Stem

Wang

Chen

et al. 2015

PLoS ONE

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Backgrounds Cistanche deserticola is a completely non-photosynthetic parasitic plant with great medicinal value and mainly distributed in desert of Northwest China. Its dried fleshy stem is a crucial tonic in traditional Chinese medicine with roles of mainly improving male sexual function and strengthening immunity, but few mechanistic studies have been conducted partly due to the lack of genomic and transcriptomic resources.ResultsIn this study, we performed deep transcriptome sequencing in fleshy stem of C. deserticola, and about 80 million reads were generated using Illumina pair-end sequencing on HiSeq2000 platform. Using trinity assembler, we obtained 95,787 transcript sequences with transcript lengths ranging from 200bp to 15,698bp, having an average length of 950 bases and the N50 length of 1,519 bases. 63,957 transcripts were identified actively expressed with FPKM ≥ 0.5, in which 30,098 transcripts were annotated with gene descriptions or gene ontology terms by sequence similarity analyses against several public databases (Uniprot, NR and Nt at NCBI, and KEGG). Furthermore, we identified key enzyme genes involved in biosynthesis of lignin and phenylethanoid glycosides (PhGs) which are known to be the primary active ingredients. Four phenylalanine ammonia-lyase (PAL) genes, the first key enzyme in lignin and PhG biosynthesis, were identified based on sequences comparison and phylogenetic analysis. Two biosynthesis pathways of PhGs were also proposed for the first time.ConclusionsIn all, we completed a global analysis of the C. deserticola fleshy stem transcriptome using RNA-seq technology. A collection of enzyme genes related to biosynthesis of lignin and phenylethanoid glysides were identified from the assembled and annotated transcripts, and the gene family of PAL was also predicted. The sequence data from this study will provide a valuable resource for conducting future phenylethanoid glysides biosynthesis researches and functional genomic studies in this important medicinal plant.

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Section: Introductionmentioning

confidence: 99%

“…To date, more than 20 phenylethanoid glycosides have been isolated from the succulent stem of C . deserticola [ 9 , 14 , 16 ]. Among them, acteoside and echinacoside are two main components with significant pharmacological activities [ 2 , 16 ], and documented as the quality standards of C .…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq Based De Novo Transcriptome Assembly and Gene Discovery of Cistanche deserticola Fleshy Stem

Wang

Chen

et al. 2015

PLoS ONE

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“…However, the reports of those found in fungi cultures are very insufficient, although some of them have biological activities against other fungi and bacteria. In plants the compound, 1,2,4,8-tetramethylbicyclic[6.3.0]undeca-2,4-diene has been detected in: pineapple cores (Chang-Bin et al 2011), Cistanche deserticola (Sarsenbayev et al 2013) and Salvia multicaulis (Karamian et al 2013). α-gurjunene occurs in Achillea tenuifolia (Shafaghat 2009), tubers of Cyperus rotundus (Bisht et al 2011), Juniperus communis (Abbassy and Marei 2013) and Blumea eriantha (Pednekar et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum

et al. 2014

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Low-molecular weight organic compounds (LMWOC) of water extracts from Cistanche deserticola stolones depending on treatment

Cited by 2 publications

References 0 publications

RNA-Seq Based De Novo Transcriptome Assembly and Gene Discovery of Cistanche deserticola Fleshy Stem

RNA-Seq Based De Novo Transcriptome Assembly and Gene Discovery of Cistanche deserticola Fleshy Stem

Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum

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