Low Molecular Weight pDMAEMA-block-pHEMA Block-Copolymers Synthesized via RAFT-Polymerization: Potential Non-Viral Gene Delivery Agents?

Samsonova, Olga; Pfeiffer, Christian; Hellmund, Markus; Merkel, Olivia M.; Kissel, Thomas

doi:10.3390/polym3020693

Cited by 75 publications

(70 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…poly(DMAEMA)-b-poly(HEMA)-dithiobenzoate were shown to have low cytotoxicity with relation to block copolymers based on polyethyleneimine. [217] poly(DMAEA)-trithiocarbonate was shown to have low cytotoxicity and to degrade under physiological conditions to poly(AA). [444] Polymerization Kinetics Kinetic simulation is frequently used as a tool to correlate experimental data with theoretical models.…”

Section: Toxicity Of Raft-synthesized Polymersmentioning

confidence: 99%

Living Radical Polymerization by the RAFT Process – A Third Update

2012

View full text Add to dashboard Cite

This paper provides a third update to the review of reversible deactivation radical polymerization (RDRP) achieved with thiocarbonylthio compounds (ZC(¼S)SR) by a mechanism of reversible addition-fragmentation chain transfer ( Chem. 2009Chem. , 62, 1402. This review cites over 700 publications that appeared during the period mid 2009 to early 2012 covering various aspects of RAFT polymerization which include reagent synthesis and properties, kinetics and mechanism of polymerization, novel polymer syntheses, and a diverse range of applications. This period has witnessed further significant developments, particularly in the areas of novel RAFT agents, techniques for end-group transformation, the production of micro/nanoparticles and modified surfaces, and biopolymer conjugates both for therapeutic and diagnostic applications.

show abstract

Section: Toxicity Of Raft-synthesized Polymersmentioning

confidence: 99%

Living Radical Polymerization by the RAFT Process – A Third Update

2012

View full text Add to dashboard Cite

show abstract

“…7,85 However, this can be also used to prevent non-specific interactions and allow specific internalisation due to targeting moieties at the same time, in particular in vivo. A frequently used approach is the combination of a PEG block and a cationic block, resulting in reduced cytotoxicity and stealth behaviour.…”

Section: Polymer Architecturementioning

confidence: 99%

“…There are multitude studies about PDMAEMAbased stars. On one hand, a higher cytotoxicity was observed when keeping the arm length constant and increasing the number of arms (4,7,14,21), thus, also increasing the molar mass. In particular, T. K. Georgiou demonstrated the benefit of PDMAEMA stars as transfection agents.…”

Section: Star-shaped Polymersmentioning

confidence: 99%

See 1 more Smart Citation

The influence of polymer architecture on in vitro pDNA transfection

et al. 2015

View full text Add to dashboard Cite

In 2012, the first gene therapy agent was approved by the Europe Medicines Agency leading to increased interest in this research field. Beside viruses, non-viral agents based on lipids or polymers represent aspiring alternatives to deliver the genetic material. Different hurdles have to be overcome depending on the kind of nucleic acid used, where plasmid DNA (pDNA) and small interfering RNA represent the common ones. The main challenge for transfection agents, in particular for pDNA delivery, is the transfer to the cell and into the cell nuclei. Within the group of transfection vesicles, cationic polymers show promising features and variability, as they can be synthesized with tailor-made physical and chemical properties (architectures and functionalisation). In the field of polymer-based gene delivery, the tuning potential of polymers by using different architectures like graft and star-shaped polymers as well as self-assembled block copolymers is immense. In particular, in the last few years numerous new polymer designs showed enhanced transfection properties in combination with good biocompatibility. Furthermore, new insights into the transfection mechanism demonstrated the continuous progress in this field. Polymer architecture influences the polyplex characteristics and the latter has an impact on the transfection mechanism, e.g. the interaction with the cellular membrane depends on the polyplex shape. Moreover, polyplex dissociation can be easily influenced by the polymer chemistry, thus biodegradable linkers lead to well suited polymers with reduced toxicity and high delivery potential, and are also promising for in vivo applications. This review focuses on the influence of polymer architectures for pDNA transfection in vitro, showing recent developments and insights. The theoretical background concerning the biological challenges for cationic polymers and the impact of graft-or star-shaped architectures as well as self-assembled structures will be presented in detail.

show abstract

“…Residual 30% of polymer chains are initiated by AIBN forming a mixture of homo-, diblock-and even triblock copolymers. Purification techniques like precipitation or dialysis do not always help to separate diblock copolymers from homopolymers [44]. Moreover, cleavage of Z groups sometimes takes place during purification by dialysis [45].…”

Section: One-pot Synthesis Of Amphiphilic Multiblock Copolymersmentioning

confidence: 99%