Low monocyte HLA-DR expression as an indicator of immunodepression in very low birth weight infants

Palojärvi, Anniina; Petäjä, Jari; Siitonen, Sanna; Janér, Cecilia; Andersson, Sture

doi:10.1038/pr.2012.199

Cited by 31 publications

(24 citation statements)

References 28 publications

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“…In VLBW infants, reduced monocyte HLA-DR expression associated with risk for infections, respiratory distress syndrome, and bronchoplastic pulmonary disease, along with a clear association with gestational age [14,15], supporting the general notion that immunodepression per se may associate with adverse outcome [25]. Our hypothesis that timing of maternal BM affects the course of neonatal HLA-DR response was confirmed.…”

Section: Discussionsupporting

confidence: 64%

“…Monocyte HLA-DR expression was measured as reported [14]. HLA-DR expression is reported as percentage of HLA-DR-positive monocytes (HLA-DR%) of total monocytes analyzed.…”

Section: Methodsmentioning

confidence: 99%

“…Recent data demonstrate that immunodepression also occurs in small preterm infants and associate with immaturity, infections and pulmonary morbidity [14] as well as with chorionamnionitis and sepsis [15]. In fetal sheep, maternal betamethasone (BM) has time-dependent immunomodulatory effects on monocytes, which alter hydrogen peroxidase and IL-6 production [16].…”

Section: Introductionmentioning

confidence: 99%

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Antenatal Betamethasone Associates with Transient Immunodepression in Very Low Birth Weight Infants

et al. 2013

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Background: Antenatal betamethasone (BM) treatment for mothers at risk for premature delivery is effective in reducing neonatal morbidity. Immunodepression, defined as monocyte human leukocyte antigen (HLA)-DR expression <60%, is common in patients in intensive care. In very low birth weight (VLBW) infants, immunodepression correlates with gestational age and may predispose to infections. Objectives: To evaluate whether timing of antenatal BM associates with immunodepression in VLBW infants. Methods: We determined monocyte HLA-DR expression by flow cytometry and measured 13 cytokines, cortisol, and BM in plasma from 56 VLBW infants. We calculated total glucocorticoid index as the sum of BM and cortisol at the ratio 33.3:1. Results: HLA-DR expression both in cord (R² = 0.175, p = 0.033, n = 26) and on day 1 (R² = 0.125, p = 0.011, n = 51) showed an association with timing of BM. A short interval from BM to birth induced more pronounced and prolonged immunodepression, with lower HLA-DR% on postnatal day 7. On day 3, 25 infants (45%) met the criteria of immunodepression. HLA-DR expression correlated negatively with total glucocorticoid index (cord: R² = -0.573, p = 0.003, n = 13; day 1: R² = -0.213, p = 0.008, n = 32). Elapsed time from maternal BM correlated positively with concentrations of cytokines IL-6 and IL-10 on day 1. Conclusions: In VLBW infants, antenatal BM associated with transient immunodepression in a time-dependent manner. Suppression of both anti- and proinflammatory cytokines occurred. These effects may lead to an increased risk for later infections.

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Section: Discussionsupporting

confidence: 64%

“…Monocyte HLA-DR expression was measured as reported [14]. HLA-DR expression is reported as percentage of HLA-DR-positive monocytes (HLA-DR%) of total monocytes analyzed.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antenatal Betamethasone Associates with Transient Immunodepression in Very Low Birth Weight Infants

et al. 2013

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“…The expression of CD64 and HLA-Dr on the different monocyte subsets of STEMI, as well as NSTEMI, was according to what is already known in the literature (2,9,(23)(24)(25) and corresponds with the nature of their function (pro-inflammatory or antigen-presenting). There were no differences in expression level of these epitopes on monocytes between patients with NSTEMI versus STEMI.…”

Section: Discussionmentioning

confidence: 77%

Intermediate and nonclassical monocytes show heterogeneity in patients with different types of acute coronary syndrome

et al. 2017

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This study was performed to gain further insight in the heterogeneity of monocytes in the different categories of acute coronary syndrome (ACS), especially between patients with unstable angina pectoris, ST-elevation myocardial infarction (STEMI), and non-ST-elevation myocardial infarction (NSTEMI). For this purpose, blood samples were collected in the acute phase from patients presenting with an ACS. These samples were examined with multiparameter flow cytometry to identify the different monocyte subsets and to analyze the expression of monocyte-associated molecules. Leukocytes, as well as an absolute number of monocytes, showed a clear and significant increase in patients with STEMI. This increase was seen in all subtypes of monocytes. The classical monocytes (CD14++CD16-) of patients with an NSTEMI had a significantly increased CD11b expression when compared to the control group, while these cells showed a decreased expression pattern in STEMI patients. This increased CD11b-expression was also seen in the intermediate monocytes of NSTEMI, while it was almost completely downregulated on the intermediate monocytes of STEMI. Finally, CX3CR1, which is almost exclusively expressed on intermediate and nonclassical monocytes, showed a significant decrease in expression in patients with STEMI. In conclusion, intermediate and nonclassical monocytes have a different immunophenotypic pattern in patients with STEMI versus NSTEMI. These differences reflect the pro-inflammatory state of the monocytes in NSTEMI and can be used as target molecules for novel therapeutic strategies to diminish the migration of proinflammatory monocytes into the myocardial tissue. © 2017 International Society for Advancement of Cytometry.

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“…[28][29][30] CD69 is generally regarded as the earliest cell surface activation marker of both umbilical cord and peripheral blood mononuclear cells induced by a mitogenic stimulus. These results seemed to be associated with impaired neonatal host defense, also contributing to the high incidence of bacterial infections, sepsis and subsequent development of BPD.…”

Section: Hla-dr Expression On Monocytes On the First Days Of Life (1 mentioning

confidence: 99%

Immune cell subsets, cytokine and cortisol levels during the first week of life in neonates born to pre‐eclamptic mothers

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et al. 2017

American J Rep Immunol

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Low monocyte HLA-DR expression as an indicator of immunodepression in very low birth weight infants

Cited by 31 publications

References 28 publications

Antenatal Betamethasone Associates with Transient Immunodepression in Very Low Birth Weight Infants

Antenatal Betamethasone Associates with Transient Immunodepression in Very Low Birth Weight Infants

Intermediate and nonclassical monocytes show heterogeneity in patients with different types of acute coronary syndrome

Immune cell subsets, cytokine and cortisol levels during the first week of life in neonates born to pre‐eclamptic mothers

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