Low-oxalate Spinach Mutant Induced by Chemical Mutagenesis

Regulation of Oxalate Metabolism in Spinach Revealed by RNA-Seq-Based Transcriptomic Analysis

“…Demonstration of a converse relationship in fast-growing spinach cultivars that accumulated low oxalates but high nitrates, and vice versa, in a study comprising 182 open-pollinated and F1 hybrid cultivars and breeding lines [ 33 ], suggests the possibility of developing fast-growing cultivars as a breeding strategy to reduce oxalate accumulation. Although no specific genes were identified, an ethyl methanesulfonate (EMS)-mediated mutant in spinach [ 34 ] and ion-beam-mutagenized rice [ 35 ] accumulating lower oxalate levels suggests the possibility of the genetic improvement of spinach for oxalate reduction. Identification of SNP markers associated with high oxalate [ 36 ] in spinach has allowed accelerating efforts to breed spinach varieties with low oxalic acid content.…”

Nitrate and oxalate in germplasm collections of spinach and other leafy vegetables

“…The mechanism of the effects of different nitrogen form on oxalate concentrations in plants is not fully understood, but several processes have been implicated (Reid and Smith, 2001). Regarding oxalate, Murakami et al (2009) have produced low-content spinach (with one third to one sixth of the oxalate levels compared to control material). This has been done by chemical mutagenesis.…”

Oxalate synthesis in leaves is associated with root uptake of nitrate and its assimilation in spinach (Spinacia oleracea L.) plants

“…Oxalate in combination with calcium results in calcium oxalate precipitation in the kidney, resulting in kidney stones in the human body . Because to its detrimental effects on human health, oxalate in leafy vegetables has caused serious concerns and many studies have been conducted in recent years with the aim of minimizing oxalate accumulation in vegetables …”